Fabrication of HA/PHBV composite scaffolds through the emulsion freezing/freeze-drying process and characterisation of the scaffolds

Sultana, Naznin; Wang, Min

doi:10.1007/s10856-007-3214-3

Cited by 147 publications

(95 citation statements)

References 15 publications

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“…The obtained n value was 0.417, which is close to the characteristic n value for Fickian diffusion (i.e., 0.43 for sphere), indicating a diffusion controlled release mechanism. The components (PBS and ethanol) of the release medium used in this work are non-solvents of PHBV, therefore, the shrinkage or swelling of the polymer is negligible, 36 which results in a diffusion controlled drug release behavior, as discussed elsewhere.…”

Section: Daidzein Release Profilesmentioning

confidence: 99%

Multilayered drug delivery coatings composed of daidzein-loaded PHBV microspheres embedded in a biodegradable polymer matrix by electrophoretic deposition

Chen

Yao

et al. 2016

J. Mater. Chem. B

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The development of drug delivery coating systems for local and long-term drug release is gaining increasing interest especially to functionalize bioinert implants with osseointegration and antibacterial properties. In this study, a biodegradable drug delivery coating platform consisting of drug-loaded PHBV microspheres embedded in an alginate-PVA matrix was fabricated by a one-step electrophoretic deposition (EPD) process. Layer by layer (LbL) deposition was exploited to generate chitosan-alginate multilayers on the EPD-produced coating to enlarge the diffusional barrier around the microspheres for controlled drug release. Daidzein, selected as a model drug due to its anti-osteoporosis properties, was pre-encapsulated in PHBV microspheres. The parameters for microsphere fabrication were optimized by an orthogonal design approach. The loading efficiency of daidzein in both the microspheres and in the deposited coatings was adjusted by varying the processing parameters during microsphere fabrication and the EPD process. The degradation of the deposited multilayers was investigated in PBS for up to 14 days. The degradation rate, surface roughness and wettability, as well as adhesion strength of the coatings during degradation were evaluated by applying a range of techniques. A controlled and sustained daidzein release was detected from both free microspheres and microsphere-containing coatings. Finally cytotoxicity and stimulatory effects of daidzein or daidzein-loaded coatings, on both MC3T3-E1 and RAW264.7 cell lines, were studied to validate the potential of the developed coatings for orthopedic applications.

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Section: Daidzein Release Profilesmentioning

confidence: 99%

Multilayered drug delivery coatings composed of daidzein-loaded PHBV microspheres embedded in a biodegradable polymer matrix by electrophoretic deposition

Chen

Yao

et al. 2016

J. Mater. Chem. B

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“…Hence, nHA is usually combined with biopolymer and organic material into a composite form to overcome the limitations of its mechanical properties. Many studies have been reported on the fabrication of composite scaffolds using nHA with biocompatible polymers, such as poly-L-lactic acid (PLLA) (Davachi et al 2016), poly-L-glycolic acid (PLGA) (Zheng et al 2013), poly(hydroxybutyrate-co-valerate) (PHBV) (Sultana and Wang 2008), cellulose (Zadegan et al 2011), PCL (Hassan et al 2014), and polyvinyl alcohol (PVA) (Sultana and Zainal 2016).…”

Section: Introductionmentioning

confidence: 99%

Characterization, drug loading and antibacterial activity of nanohydroxyapatite/polycaprolactone (nHA/PCL) electrospun membrane

Hassan

Sultana

2017

3 Biotech

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Considering the important factor of bioactive nanohydoxyapatite (nHA) to enhance osteoconductivity or bone-bonding capacity, nHA was incorporated into an electrospun polycaprolactone (PCL) membrane using electrospinning techniques. The viscosity of the PCL and nHA/PCL with different concentrations of nHA was measured and the morphology of the electrospun membranes was compared using a field emission scanning electron microscopy. The water contact angle of the nanofiber determined the wettability of the membranes of different concentrations. The surface roughness of the electrospun nanofibers fabricated from pure PCL and nHA/PCL was determined and compared using atomic force microscopy. Attenuated total reflectance Fourier transform infrared spectroscopy was used to study the chemical bonding of the composite electrospun nanofibers. Beadless nanofibers were achieved after the incorporation of nHA with a diameter of 200-700 nm. Results showed that the fiber diameter and the surface roughness of electrospun nanofibers were significantly increased after the incorporation of nHA. In contrast, the water contact angle (132°± 3.5°) was reduced for PCL membrane after addition of 10% (w/ w) nHA (112°± 3.0°). Ultimate tensile strengths of PCL membrane and 10% (w/w) nHA/PCL membrane were 25.02 ± 2.3 and 18.5 ± 4.4 MPa. A model drug tetracycline hydrochloride was successfully loaded in the membrane and the membrane demonstrated good antibacterial effects against the growth of bacteria by showing inhibition zone for E. coli (2.53 ± 0.06 cm) and B. cereus (2.87 ± 0.06 cm).

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“…In recent decades a variety of bone scaffold manufacturing techniques have been developed in an effort to address these limitations. Using ceramics, polymers or combinations of the two, solvent casting and particulate leaching [2], gas foaming [3], fibre meshes and fibre bonding [4], phase separation [2], melt moulding [5], emulsion freeze drying [6], solution casting [7], freeze drying [4], fused deposition modelling [8], rapid prototyping [9], membrane lamination [10], microsphere sintering [2], supercritical fluid technology [11] and various preform replication techniques [12][13][14][15][16][17][18][19][20][21][22] have all been employed to produce scaffolds with variable pore size, porosity and connectivity.…”

Section: Introductionmentioning

confidence: 99%

Comparative Characterisation of 3-D Hydroxyapatite Scaffolds Developed Via Replication of Synthetic Polymer Foams and Natural Marine Sponges

Cunningham¹,

Dunne²

2011

J Tissue Sci Eng

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The production of complex inorganic forms, based on naturally occurring scaffolds offers an exciting avenue for the construction of a new generation of ceramic-based bone substitute scaffolds. The following study reports an investigation into the architecture (porosity, pore size distribution, pore interconnectivity and permeability), mechanical properties and cytotoxic response of hydroxyapatite bone substitutes produced using synthetic polymer foam and natural marine sponge performs. Infiltration of polyurethane foam (60 pores/in2) using a high solid content (80wt %), low viscosity (0.126Pas) hydroxyapatite slurry yielded 84-91% porous replica scaffolds with pore sizes ranging from 50μm -1000μm (average pore size 577μm), 99.99% pore interconnectivity and a permeability value of 46.4 x10-10m2. Infiltration of the natural marine sponge, Spongia agaricina, yielded scaffolds with 56-61% porosity, with 40% of pores between 0-50μm, 60% of pores between 50-500μm (average pore size 349 μm), 99.9% pore interconnectivity and a permeability value of 16.8 x10-10m2. The average compressive strengths and compressive moduli of the natural polymer foam and marine sponge replicas were 2.46±1.43MPa/0.099±0.014GPa and 8.4±0.83MPa /0.16±0.016GPa respectively.Cytotoxic response proved encouraging for the HA Spongia agaricina scaffolds; after 7 days in culture medium the scaffolds exhibited endothelial cells (HUVEC and HDMEC) and osteoblast (MG63) attachment, proliferation on the scaffold surface and penetration into the pores.It is proposed that the use of Spongia agaricina as a precursor material allows for the reliable and repeatable production of ceramic-based 3-D tissue engineered scaffolds exhibiting the desired architectural and mechanical characteristics for use as a bone 3 scaffold material. Moreover, the Spongia agaricina scaffolds produced exhibit no adverse cytotoxic response.

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Fabrication of HA/PHBV composite scaffolds through the emulsion freezing/freeze-drying process and characterisation of the scaffolds

Cited by 147 publications

References 15 publications

Multilayered drug delivery coatings composed of daidzein-loaded PHBV microspheres embedded in a biodegradable polymer matrix by electrophoretic deposition

Multilayered drug delivery coatings composed of daidzein-loaded PHBV microspheres embedded in a biodegradable polymer matrix by electrophoretic deposition

Characterization, drug loading and antibacterial activity of nanohydroxyapatite/polycaprolactone (nHA/PCL) electrospun membrane

Comparative Characterisation of 3-D Hydroxyapatite Scaffolds Developed Via Replication of Synthetic Polymer Foams and Natural Marine Sponges

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