Characterization, drug loading and antibacterial activity of nanohydroxyapatite/polycaprolactone (nHA/PCL) electrospun membrane

Hassan, Muhammad Imzan; Sultana, Naznin

doi:10.1007/s13205-017-0889-0

Cited by 62 publications

(29 citation statements)

References 40 publications

(46 reference statements)

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“…Whereas the roughness value obtained for the other two PCL/gelatin/P188 composites with 5% and 10% P188 were 679 ± 33 nm and 683 ± 95 nm, respectively. Although the increase in fiber diameter is known to increase the surface roughness [51][52][53], the high roughness of the scaffold with 20% P188 was probably due to increasing in P188 concentration. As 20% P188 based composite scaffold electrospun at 22 kv had the lowest average diameter of 306 ± 163 nm when compared to scaffolds with 10% and 5% P188, which had an average diameter of 327 ± 196 nm and 390 ± 158 nm, respectively ( Table 2).…”

Section: Discussionmentioning

confidence: 98%

Engineering Biomimetic Gelatin Based Nanostructures as Synthetic Substrates for Cell Culture

2019

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There is a need for synthetic substrates that replicate the natural environment for in vitro intestinal models. Electrospinning is one of the most versatile and cost-effective techniques to produce nanofibrous scaffolds mimicking the basement membrane topography. In this study, three different novel electrospun nanofibrous scaffolds made of a polycaprolactone (PCL), gelatin, and poloxamer 188 (P188) blend were produced and compared with PCL and PCL/gelatin fibers produced using the same solvent system and electrospinning parameters. Each polymer solution used in this experiment was electrospun at four different voltages to study its influence on fiber diameter. The morphology and physical characteristics of the fibers were studied using scanning electron microscopy and atomic force microscopy. The average fiber diameter of all scaffolds was within 200–600 nm and no significant decrease in diameter with an increase in voltage was observed. Attenuated total reflection Fourier transform infrared spectroscopy was used to determine the chemical characteristics of the nanofibrous scaffold. The conductivity of the polymer solutions was also analyzed. Biocompatibility of the scaffolds was determined by a cell proliferation study performed using colorectal carcinoma (Caco-2) cells. PCL/gelatin/P188 scaffolds exhibited higher cell proliferation compared to PCL, PCL/gelatin scaffolds, and the control (tissue culture multi-well plate) with PCL/gelatin/P188 80:10:10 sample showing the highest cell proliferation.

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Section: Discussionmentioning

confidence: 98%

Engineering Biomimetic Gelatin Based Nanostructures as Synthetic Substrates for Cell Culture

2019

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“…nHA is the major inorganic component of the bone matrix, and its specific affinity toward many adhesive proteins and direct involvement in the bone cell differentiation and mineralization processes make nHA especially appealing for applications in the bone regeneration field [ 62 ]. nHA has been incorporated in PCL by electrospinning in several studies in vitro [ 63 ] and in vivo in a calvarial defect mouse model where association with HA significantly improved bone healing induced by PCL [ 64 ]. The HA-coated PCL membrane has favorable effects on proliferation and differentiation of human periodontal ligament cells and might be a candidate material for periodontal tissue regeneration [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

Synthesis of a Novel Electrospun Polycaprolactone Scaffold Functionalized with Ibuprofen for Periodontal Regeneration: An In Vitro andIn Vivo Study

et al. 2018

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Ibuprofen (IBU) has been shown to improve periodontal treatment outcomes. The aim of this study was to develop a new anti-inflammatory scaffold by functionalizing an electrospun nanofibrous poly-ε-caprolactone membrane with IBU (IBU-PCL) and to evaluate its impact on periodontal inflammation, wound healing and regeneration in vitro and in vivo. IBU-PCL was synthesized through electrospinning. The effects of IBU-PCL on the proliferation and migration of epithelial cells (EC) and fibroblasts (FB) exposed to Porphyromonas gingivlais lipopolysaccharide (Pg-LPS) were evaluated through the AlamarBlue test and scratch assay, respectively. Anti-inflammatory and remodeling properties were investigated through Real time qPCR. Finally, the in vivo efficacy of the IBU-PCL membrane was assessed in an experimental periodontitis mouse model through histomorphometric analysis. The results showed that the anti-inflammatory effects of IBU on gingival cells were effectively amplified using the functionalized membrane. IBU-PCL reduced the proliferation and migration of cells challenged by Pg-LPS, as well as the expression of fibronectin-1, collagen-IV, integrin α3β1 and laminin-5. In vivo, the membranes significantly improved the clinical attachment and IBU-PCL also reduced inflammation-induced bone destruction. These data showed that the IBU-PCL membrane could efficiently and differentially control inflammatory and migratory gingival cell responses and potentially promote periodontal regeneration.

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“…The O─C vibrations of PCL were observed at the absorption peak of 1053, 1018, and 923 cm −1 with the CH 2 vibration appearing at around 809 cm −1 . The ester COO vibrations occur at 1159 and 1238 cm −1 . From these results, it can be concluded that the CQ‐PCL NPs loaded CS/Col/HA was successfully incorporated into the scaffolds.…”

Section: Resultsmentioning

confidence: 75%

Fabrication of chitosan/collagen/hydroxyapatite scaffolds with encapsulated Cissus quadrangularis extract

Thongtham

Chai‐in

Unger

et al. 2020

Polymers for Advanced Techs

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Here, we demonstrated the fabrication of a composite scaffold (chitosan [CS], collagen [Col], and hydroxyapatite [HA]) with the incorporation of encapsulated Cissus quadrangularis (CQ) extract for tissue engineering applications. First, the crude extract of CQ loaded nanoparticles were synthesized via double emulsion technique using polycaprolactone (PCL) and polyvinyl alcohol (PVA) as oil and aqueous phases, respectively. Both PCL (20, 40, and 80 mg/mL) and PVA (0.5%, 1%, and 3% w/v) concentrations were varied to determine the optimum concentrations for CQ-loaded nanoparticle preparation. The CQ-loaded PCL nanoparticles (CQ-PCL NPs), prepared with 20 mg/mL PCL and 0.5% (w/v) PVA, exhibited the smallest size of 334.22 ± 43.21 nm with 95.54 ± 1.49% encapsulation efficiency. Then, the CQ-PCL NPs were incorporated into the CS/Col/HA scaffolds. These scaffolds were also studied for their ultrastructure, pore sizes, chemical composition, compressive modulus, water swelling, weight loss, and biocompatibility. The results showed that the addition of CQ-PCL NPs into the scaffolds did not dramatically alter the ultrastructure and properties of the scaffolds, compared to CS/Col/HA scaffolds alone. However, incorporation of CQ-PCL NPs in the scaffolds improved the release profile of CQ by preventing the initial burst release and prolonging the release rate of CQ. In addition, the CQ-PCL NPs-loaded CS/Col/HA scaffolds supported the attachment and proliferation of MC3T3-E1 osteoblast cells.

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Characterization, drug loading and antibacterial activity of nanohydroxyapatite/polycaprolactone (nHA/PCL) electrospun membrane

Cited by 62 publications

References 40 publications

Engineering Biomimetic Gelatin Based Nanostructures as Synthetic Substrates for Cell Culture

Engineering Biomimetic Gelatin Based Nanostructures as Synthetic Substrates for Cell Culture

Synthesis of a Novel Electrospun Polycaprolactone Scaffold Functionalized with Ibuprofen for Periodontal Regeneration: An In Vitro andIn Vivo Study

Fabrication of chitosan/collagen/hydroxyapatite scaffolds with encapsulated Cissus quadrangularis extract

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