Fabrication of Graphene Oxide and Nanohydroxyapatite Reinforced Gelatin–Alginate Nanocomposite Scaffold for Bone Tissue Regeneration

Abstract

Graphene oxide (GO) and nanohydroxyapatite (nHAp) proved to be a potential material for bone tissue-regeneration applications. Therefore, GO and nHAp reinforced porous polymeric nanocomposite scaffolds have been gaining significant research thrust. In this study, GO and nHAp based nanocomposite was synthesized and used as a reinforcing agent to develop gelatin-alginate (GA)-based three-dimensional porous polymeric nanocomposite scaffold. The polymeric nanocomposite scaffold (nHAp-GO/GA) was fabricated by using freeze-drying process, which may show a synergistic effect of each of the components for tissue regeneration. The scaffold demonstrates good physico-chemical properties. The porous microstructure of the scaffold is evidenced by Field emission scanning electron microscopy (FESEM). High swelling of the scaffold in presence water, indicates that the scaffold is highly hydrophilic, which implies the suitability of the scaffold for tissue regeneration. Further, the incorporation of nHAp-GO enhances the compressive strength of the scaffold while reduces the rate of biodegradation, which is good for bone tissue engineering. The scaffold is biocompatible. In vitro cell study with MG-63 bone cells demonstrates the synergistic effect of each of the components of the scaffold, on mineral deposition. Therefore, it can be concluded that nHAp-GO/GA polymeric nanocomposite scaffold can be a potential candidate for bone tissue regeneration.

Antiviral performance of graphene-based materials with emphasis on COVID-19: A review

“…These unique properties of graphene and its derivatives have made these nanostructured materials suitable for various biological and medical applications [168] including anti-pathogenic applications [169] , [170] , [171] , [172] , [173] , [174] , [175] , [176] , [177] , biosensors [178] , [179] , [180] , [181] , [182] , [183] , [184] , bioimaging [185] , [186] , [187] , [188] , [189] , [190] , [191] , [192] , tissue engineering [193] , [194] , [195] , [196] , drug delivery [197] , [198] , [199] , [200] , [201] . Therefore, the interactions between graphene materials and viruses should be of great interest.…”

Differently Charged P (VDF-TrFE) Membranes Influence Osteogenesis Through Differential Immunomodulatory Function of Macrophages

“…To clarify whether the CM from macrophage-P (VDF-TrFE) culture modulates osteogenic differentiation, alkaline phosphatase (ALP) staining and gene expression analysis were performed after 7 days of culture, as reported before (Purohit et al, 2020). In short, the cells were rinsed three times in PBS, fixed with 4% paraformaldehyde for 10 min, then incubated in ALP staining solution (Beyotime Biotechnology, Shanghai, China) at room temperature for 1 h. The gene expression was evaluated as described in paragraph 2.2.6 and the primers used are listed in Table 1.…”

Efficacy of Bacterial Nanocellulose in Hard Tissue Regeneration: A Review

“…Alginate (Alg) hydrogels have widely been used in biomedical applications but show unstable and poor mechanical strength as well as lack of cell-recognition active sites for tissue engineering, specifically bone tissue regeneration [125][126][127]. Therefore, Yan et al 2017 applied BNC to develop Alg/BNC-CS-Gel-based composite scaffold.…”