Fabrication of D–A–D type conducting polymer, carbon nanotubes and silica nanoparticle-based laccase biosensor for catechol detection

Deniz, Sıddıka Aybüke; Goker, Seza; Toppare, Levent; Söylemez, Saniye

doi:10.1039/d2nj02147a

Cited by 4 publications

(4 citation statements)

References 32 publications

(43 reference statements)

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“…For example, the most effective ABS, containing the redox mediator CuCo, showed a 51-fold increase in sensitivity in comparison with the control ABS. This is significantly higher than the sensitivity reported earlier for laccase-based ABSs [ 38 , 49 , 50 ], except for our recent work [ 19 ], where we described the electroactive CuCeNPs coupled with porous gold as a platform for the construction of oxidase-based ABSs. We have demonstrated here the possibility to improve the selectivity of ABS to catechol in the mixture with several non-phenolic substrates of laccase.…”

Section: Discussionmentioning

confidence: 55%

“…Fabrication of amperometric (bio)sensors for fast, sensitive, and, at the same time, selective assay of catechol (or any other individual substrate of laccase) is a serious challenge for electrochemists today. Many reports concerning specific catechol detection and describing laccase-based biosensors [ 28 , 36 , 37 , 38 ] as well as non-enzymatic sensors have been published [ 27 , 33 , 34 , 35 , 39 , 40 , 41 , 42 ]. All the reported methods are based on using advanced nanomaterials.…”

Section: Introductionmentioning

confidence: 99%

“…All the reported methods are based on using advanced nanomaterials. It was demonstrated that selectivity of laccase-based methods depended on the producer of the enzyme [ 2 , 9 , 11 , 12 , 20 ], the mode of enzyme immobilization/fixation [ 23 , 28 ], the nature of nanomaterials and composition of a reaction mixture [ 31 , 36 , 37 , 38 ], working potential [ 39 ], presence of organic solvents [ 43 ], and many other parameters.…”

Section: Introductionmentioning

confidence: 99%

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Nanomaterials as Redox Mediators in Laccase-Based Amperometric Biosensors for Catechol Assay

et al. 2022

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Laccase is a copper-containing enzyme that does not require hydrogen peroxide as a co-substrate or additional cofactors for an enzymatic reaction. Nanomaterials of various chemical structures are usually applied to the construction of enzyme-based biosensors. Metals, metal oxides, semiconductors, and composite NPs perform various functions in electrochemical transformation schemes as a platform for the enzyme immobilization, a mediator of an electron transfer, and a signal amplifier. We describe here the development of amperometric biosensors (ABSs) based on laccase and redox-active micro/nanoparticles (hereafter—NPs), which were immobilized on a graphite electrode (GE). For this purpose, we isolated a highly purified enzyme from the fungus Trametes zonatus, and then synthesized bi- and trimetallic NPs of noble and transition metals, as well as hexacyanoferrates (HCF) of noble metals; these were layered onto the surfaces of GEs. The electroactivity of many of the NPs immobilized on the GEs was characterized by cyclic voltammetry (CV) experiments. The most effective mediators of electron transfer were selected as the platform for the development of laccase-based ABSs. As a result, a number of catechol-sensitive ABSs were constructed and characterized. The laccase/CuCo/GE was demonstrated to possess the highest sensitivity to catechol (4523 A·M−1·m−2) among the tested ABSs. The proposed ABSs may be promising for the analysis of phenolic derivatives in real samples of drinking water, wastewater, and food products.

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Section: Discussionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nanomaterials as Redox Mediators in Laccase-Based Amperometric Biosensors for Catechol Assay

et al. 2022

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“…In addition to providing a biocompatible environment for enzymes, silica nanoparticles (SiO 2 -NPs) can improve the sensitivity of biosensors by promoting the electrical connection between the electrode surface and biomolecules [76]. Mathelié-Guinlet et al [77] designed an electrochemical immuno-biosensor based on two-step spin-coating process to immobilize SiO 2 -NPs.…”

Section: Inorganic-based Nanomaterialsmentioning

confidence: 99%

Optically Active Nanomaterials and Its Biosensing Applications—A Review

et al. 2023

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This article discusses optically active nanomaterials and their optical biosensing applications. In addition to enhancing their sensitivity, these nanomaterials also increase their biocompatibility. For this reason, nanomaterials, particularly those based on their chemical compositions, such as carbon-based nanomaterials, inorganic-based nanomaterials, organic-based nanomaterials, and composite-based nanomaterials for biosensing applications are investigated thoroughly. These nanomaterials are used extensively in the field of fiber optic biosensing to improve response time, detection limit, and nature of specificity. Consequently, this article describes contemporary and application-based research that will be of great use to researchers in the nanomaterial-based optical sensing field. The difficulties encountered during the synthesis, characterization, and application of nanomaterials are also enumerated, and their future prospects are outlined for the reader’s benefit.

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Electrochemical sensors

Keles,

Oktay,

Aslan

et al. 2024

Sensory Polymers

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Fabrication of D–A–D type conducting polymer, carbon nanotubes and silica nanoparticle-based laccase biosensor for catechol detection

Abstract: A sensing platform for catechol detection incorporating triple key materials based on SiNPs, D–A–D type conducting polymer, and MWCNTs.

Cited by 4 publications

References 32 publications

Nanomaterials as Redox Mediators in Laccase-Based Amperometric Biosensors for Catechol Assay

Nanomaterials as Redox Mediators in Laccase-Based Amperometric Biosensors for Catechol Assay

Optically Active Nanomaterials and Its Biosensing Applications—A Review

Electrochemical sensors

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