Fabrication of conductive NGF-conjugated polypyrrole–poly(l-lactic acid) fibers and their effect on neurite outgrowth

Zeng, Jingwen; Huang, Zhongbing; Yin, Guangfu; Qin, Jiabang; Chen, Xianchun; Gu, Jianwen

doi:10.1016/j.colsurfb.2013.05.012

Cited by 42 publications

(38 citation statements)

References 45 publications

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“…The average neurite length of the PC12 cells on PPy-APFs was ca. 111 m, which was significantly larger than those on PPy-AFs and PPy-RFs (about 72 m and 76 m, respectively), suggesting that porous structure on PPy-APFs improved the neurite outgrowth because it could enhance neurite adhesion on PPy-APFs [8,31]. These results indicated that PPy-APFs could not only guide neurite growth along the fiber axis but also enhance the rate of neurite extension.…”

Section: Contact Guidance Of Ppy-apfs On Pc12 Cell Neuritesmentioning

confidence: 55%

Fabrication of aligned, porous and conductive fibers and their effects on cell adhesion and guidance

Yang

Huang

Yin

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Section: Contact Guidance Of Ppy-apfs On Pc12 Cell Neuritesmentioning

confidence: 55%

Fabrication of aligned, porous and conductive fibers and their effects on cell adhesion and guidance

Yang

Huang

Yin

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…124 Zeng et al effectively applied ES to PPY scaffolds with rough scaffold surface containing NGF that led to accelerated axon elongation. 18 The study also demonstrated the importance of selecting an appropriate level of ES. Scaffolds seeded with PC12 cells were electrically stimulated at different voltages (0, 10, 100, or 1000 mV/cm) for 2 h by a constant voltage source.…”

Section: Conductive Polymers For Nerve Growth Conduitsmentioning

confidence: 89%

“…Most commonly, PC12 cells (a neural crest-derived cell line that comes from rats) have been shown to benefit from ES through scaffolds that incorporate PPY to promote conductivity. 13–18 Other studies have used dorsal root ganglia cells in order to show neurite outgrowth through ES of PPY. 19–21 Polypyrrole scaffolds were able to support Schwann cell adhesion, survival, migration, and proliferation, and neurites outgrowth.…”

Section: Conductive Polymers For Nerve Growth Conduitsmentioning

confidence: 99%

Peripheral Nerve Regeneration Strategies: Electrically Stimulating Polymer Based Nerve Growth Conduits

Anderson

Shelke

Manoukian

et al. 2015

Crit Rev Biomed Eng

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Treatment of large peripheral nerve damages ranges from the use of an autologous nerve graft to a synthetic nerve growth conduit. Biological grafts, in spite of many merits, show several limitations in terms of availability and donor site morbidity, and outcomes are suboptimal due to fascicle mismatch, scarring, and fibrosis. Tissue engineered nerve graft substitutes utilize polymeric conduits in conjunction with cues both chemical and physical, cells alone and or in combination. The chemical and physical cues delivered through polymeric conduits play an important role and drive tissue regeneration. Electrical stimulation (ES) has been applied toward the repair and regeneration of various tissues such as muscle, tendon, nerve, and articular tissue both in laboratory and clinical settings. The underlying mechanisms that regulate cellular activities such as cell adhesion, proliferation, cell migration, protein production, and tissue regeneration following ES is not fully understood. Polymeric constructs that can carry the electrical stimulation along the length of the scaffold have been developed and characterized for possible nerve regeneration applications. We discuss the use of electrically conductive polymers and associated cell interaction, biocompatibility, tissue regeneration, and recent basic research for nerve regeneration. In conclusion, a multifunctional combinatorial device comprised of biomaterial, structural, functional, cellular, and molecular aspects may be the best way forward for effective peripheral nerve regeneration.

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“…Some studies on the combination of ES and CPs to promote the in vitro neurite growth had been reported (S. J. Lee et al, ; Schmidt et al, ; Zeng et al, ). However, due to the short‐time conductivity of CPs, there were few reports about the in vivo nerve repair with CPs under ES.…”

Section: Introductionmentioning

confidence: 99%

Preparation of carboxylic graphene oxide‐composited polypyrrole conduits and their effect on sciatic nerve repair under electrical stimulation

Chen

Liu

Huang

et al. 2019

J Biomedical Materials Res

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Carboxylic graphene oxide‐composited polypyrrole/poly‐l‐lactic acid (C‐GO/PPy/PLLA) films were fabricated by electrochemical deposition of C‐GO‐composited PPy on PLLA fibers‐film, and their conductivity and tensile strength (∼4.6 S/cm and 26.4 MPa, respectively) were stably remained after the immersion of 4 weeks, due to the hydrogen bond interaction between graphene oxide's carboxylic groups and pyrrole's imino groups. Their specific surface areas of ∼57.5 m2/g and pore volume of ∼0.02 cm3/g were significantly larger than those of PPy/PLLA films, due to the addition of C‐GO nanosheets. Then, C‐GO/PPy/PLLA conducting conduit with 2 mm inner diameter was prepared to bridge 10 mm sciatic nerve defect of rats, and the direction of fiber‐axis in the conduit was the same as the conduit central axis. Electrical stimulation (ES) of 1 V and 20 Hz through the conducting conduit was exerted on the defect site. The results of in vivo electrophysiological and histological evaluation indicated that, the sciatic nerve defect could be repaired in C‐GO/PPy/PLLA conduit, moreover the re‐innervated gastrocnemius muscle and nerve conduction in C‐GO/PPy/PLLA conduit & ES group were obviously better than the conduit without ES group. The results of transmission electron microscope analysis also demonstrated that the mean thickness of myelin sheath and diameter of axon in C‐GO/PPy/PLLA conduit & ES group were significantly larger than those without ES, suggesting that the repair efficiency of ES & conduit group was closer to that of autograft group. These results indicated the great potential of C‐GO/PPy/PLLA with the in vivo ES in the application of sciatic nerve repair.

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Fabrication of conductive NGF-conjugated polypyrrole–poly(l-lactic acid) fibers and their effect on neurite outgrowth

Cited by 42 publications

References 45 publications

Fabrication of aligned, porous and conductive fibers and their effects on cell adhesion and guidance

Fabrication of aligned, porous and conductive fibers and their effects on cell adhesion and guidance

Peripheral Nerve Regeneration Strategies: Electrically Stimulating Polymer Based Nerve Growth Conduits

Preparation of carboxylic graphene oxide‐composited polypyrrole conduits and their effect on sciatic nerve repair under electrical stimulation

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