Fabrication of collagen membranes with different intrafibrillar mineralization degree as a potential use for GBR

Wang, Jing; Qu, Yinan; Chen, Chaoqun; Sun, Jian; Pan, Haihua; Shao, Changyu; Tang, Ruikang; Xiao, Gu

doi:10.1016/j.msec.2019.109959

Cited by 30 publications

(29 citation statements)

References 76 publications

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“…However, previous EMC material was commonly characterized by irregular HA deposition. Compared with EMC, the HIMC membrane was shown to more successfully simulate the nanostructure of natural bone through intrafibrillar mineralization and have a better ability to induce osteogenesis (Liu et al, 2016;Wang et al, 2019). According to the previous research, intrafibrillar mineralization is the main structural source of the biomechanical properties of bone and affects the biological activity of relevant cell types (Balooch et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Hierarchical Intrafibrillarly Mineralized Collagen Membrane Promotes Guided Bone Regeneration and Regulates M2 Macrophage Polarization

Xuan

et al. 2022

Front. Bioeng. Biotechnol.

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Mineralized collagen has been introduced as a promising barrier membrane material for guided bone regeneration (GBR) due to its biomimetic nanostructure. Immune interaction between materials and host significantly influences the outcome of GBR. However, current barrier membranes are insufficient for clinical application due to limited mechanical or osteoimmunomodulatory properties. In this study, we fabricated hierarchical intrafibrillarly mineralized collagen (HIMC) membrane, comparing with collagen (COL) and extrafibrillarly mineralized collagen (EMC) membranes, HIMC membrane exhibited preferable physicochemical properties by mimicking the nanostructure of natural bone. Bone marrow mesenchymal stem cells (BMSCs) seeded on HIMC membrane showed superior proliferation, adhesion, and osteogenic differentiation capacity. HIMC membrane induced CD206+Arg-1+ M2 macrophage polarization, which in turn promoted more BMSCs migration. In rat skull defects, HIMC membrane promoted the regeneration of new bone with more bone mass and more mature bone architecture. The expression levels of Runx2 and osterix and CD68 + CD206 + M2 macrophage polarization were significantly enhanced. HIMC membrane provides an appropriate osteoimmune microenvironment to promote GBR and represents a promising material for further clinical application.

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Section: Discussionmentioning

confidence: 99%

Hierarchical Intrafibrillarly Mineralized Collagen Membrane Promotes Guided Bone Regeneration and Regulates M2 Macrophage Polarization

Xuan

et al. 2022

Front. Bioeng. Biotechnol.

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“…Besides the possible usage for bone grafts, the PILP process could also be used in the guided bone regeneration (GBR) and osteoporosis field. Wang et al (2019) utilized homologous PILP processes and produced biomineralized membranes. The membranes are biocompatible with a high-stress strength, and could also promote the proliferation of bone cells.…”

Section: Bionic Applicationsmentioning

confidence: 99%

Liquid-Liquid Phase Separation in Nucleation Process of Biomineralization

Qin

Zhang

2022

Front. Chem.

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Biomineralization is a typical interdisciplinary subject attracting biologists, chemists, and geologists to figure out its potential mechanism. A mounting number of studies have revealed that the classical nucleation theory is not suitable for all nucleation process of biominerals, and phase-separated structures such as polymer-induced liquid precursors (PILPs) play essential roles in the non-classical nucleation processes. These structures are able to play diverse roles biologically or pathologically, and could also give inspiring clues to bionic applications. However, a lot of confusion and dispute occurred due to the intricacy and interdisciplinary nature of liquid precursors. Researchers in different fields may have different opinions because the terminology and current state of understanding is not common knowledge. As a result, our team reviewed the most recent articles focusing on the nucleation processes of various biominerals to clarify the state-of-the-art understanding of some essential concepts and guide the newcomers to enter this intricate but charming field.

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“…To mimic the nanostructure of mineralized collagen fibrils, the polymer-induced liquid-precursor (PILP) process [ 15 ] uses polyelectrolytes or proteins, such as polyaspartic acid (pAsp), polyacrylic acid (PAA), poly(allylamine) hydrochloride (PAH), carboxymethyl chitosan (CMC) or osteopontin (OPN), to induce the intrafibrillar mineralization of collagen fibrils [ [16] , [17] , [18] , [19] , [20] , [21] ]. PILP-mineralized collagen scaffolds have been demonstrated to promote differentiation of preosteoblasts or bone marrow mesenchymal stem cells in vitro and accelerate bone regeneration in rodent calvarial bone defects in vivo [ 20 , 22 , 23 ]. PILP can also be used to repair dentin defects [ 24 ] or fabricate bone-like models to study bone physiology and disease [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Biomimetic mineralized hybrid scaffolds with antimicrobial peptides

Zhu

Mutreja

et al. 2021

Bioactive Materials

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Infection in hard tissue regeneration is a clinically-relevant challenge. Development of scaffolds with dual function for promoting bone/dental tissue growth and preventing bacterial infections is a critical need in the field. Here we fabricated hybrid scaffolds by intrafibrillar-mineralization of collagen using a biomimetic process and subsequently coating the scaffold with an antimicrobial designer peptide with cationic and amphipathic properties. The highly hydrophilic mineralized collagen scaffolds provided an ideal substrate to form a dense and stable coating of the antimicrobial peptides. The amount of hydroxyapatite in the mineralized fibers modulated the rheological behavior of the scaffolds with no influence on the amount of recruited peptides and the resulting increase in hydrophobicity. The developed scaffolds were potent by contact killing of Gram-negative Escherichia coli and Gram-positive Streptococcus gordonii as well as cytocompatible to human bone marrow-derived mesenchymal stromal cells. The process of scaffold fabrication is versatile and can be used to control mineral load and/or intrafibrillar-mineralized scaffolds made of other biopolymers.

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Fabrication of collagen membranes with different intrafibrillar mineralization degree as a potential use for GBR

Cited by 30 publications

References 76 publications

Hierarchical Intrafibrillarly Mineralized Collagen Membrane Promotes Guided Bone Regeneration and Regulates M2 Macrophage Polarization

Hierarchical Intrafibrillarly Mineralized Collagen Membrane Promotes Guided Bone Regeneration and Regulates M2 Macrophage Polarization

Liquid-Liquid Phase Separation in Nucleation Process of Biomineralization

Biomimetic mineralized hybrid scaffolds with antimicrobial peptides

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