Fabrication of collagen-coated biodegradable polymer nanofiber mesh and its potential for endothelial cells growth

He, Wei; Ma, Zuwei; Tan, Yong Zen; Teo, Wee Eong; Ramakrishna, Seeram

doi:10.1016/j.biomaterials.2005.05.049

Cited by 437 publications

(290 citation statements)

References 23 publications

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“…The solvent located in the solvent-rich phase is subsequently removed by sublimation, leaving a porous polymer scaffold [104][105][106]. Scaffold morphology is controlled by the type of solvent, polymer concentration and phase separation temperature.…”

Section: Thermally Induced Phase Separationmentioning

confidence: 99%

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Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Gualandi

2011

Springer Theses

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and some lactide copolymers) and of non-commercial polyesters (i.e. poly-ω-pentadecalactone and some of its copolymers) were explored and discussed.Two techniques were employed to fabricate scaffolds: supercritical carbon dioxide (scCO 2 ) foaming and electrospinning (ES). The former is a powerful technology that enables to produce 3D microporous foams by avoiding the use of solvents that can be toxic to mammalian cells. The scCO 2 process, which is II commonly applied to amorphous polymers, was successfully modified to foam a highly crystalline poly(ω-pentadecalactone-co-ε-caprolactone) copolymer and the effect of process parameters on scaffold morphology and thermo-mechanical properties was investigated.In the course of the present research activity, sub-micrometric fibrous nonwoven meshes were produced using ES technology. Electrospun materials are considered highly promising scaffolds because they resemble the 3D organization of native extra cellular matrix. A careful control of process parameters allowed to fabricate defect-free fibres with diameters ranging from hundreds of nanometers to several microns, having either smooth or porous surface. Moreover, versatility of ES technology enabled to produce electrospun scaffolds from different polyesters as well as "composite" non-woven meshes by concomitantly electrospinning different fibres in terms of both fibre morphology and polymer material. The 3D-architecture of the electrospun scaffolds fabricated in this research was controlled in terms of mutual fibre orientation by properly modifying the instrumental apparatus. This aspect is particularly interesting since the micro/nano-architecture of the scaffold is known to affect cell behaviour.Since last generation scaffolds are expected to induce specific cell response, the present research activity also explored the possibility to produce electrospun scaffolds bioactive towards cells. Bio-functionalized substrates were obtained by loading polymer fibres with growth factors (i.e. biomolecules that elicit specific cell behaviour) and it was demonstrated that, despite the high voltages applied during electrospinning, the growth factor retains its biological activity once

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Section: Thermally Induced Phase Separationmentioning

confidence: 99%

“…(ii) physical absorption of biomolecules: a variety of ECM components such as gelatin, collagen, laminin and fibronectin have been physically immobilized at ES fibre surface, generally after pre-treatement with plasma [106,107].…”

Section: Surface Functionalizationmentioning

confidence: 99%

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Gualandi

2011

Springer Theses

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“…The advantage of combining a synthetic bioresorbable polymer with an ECM protein coating for EC attachment and functioning was demonstrated by several authors [42][43][44][45]. Improved endothelial cell lining of PET prosthesis modified with ECM proteins was also reported [46][47][48][49].…”

Section: Discussionmentioning

confidence: 99%

“…The mismatch in visco-elastic properties between the native artery and the rigid Dacron graft contributes to intimal hyperplasia and graft failure. Integrating synthetic and natural degradable polymers into the vascular graft wall might endow the novel grafts with more appropriate tensile and elastic mechanical properties [23,24,43].…”

Section: Discussionmentioning

confidence: 99%

Endothelial Cell Lining of PET Vascular Prostheses: Modification with Degradable Polyester-based Copolymers and Adhesive Protein Multi-layers

Filova¹

2014

J Tissue Sci Eng

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“…The simple coating works for most synthetic polymers, but the efficiency and effectiveness of the coating are dependent on polymer surface properties such as charge, chemistry and geometry. Collagen type I has been coated on PCL [142] and P(LLA-CL) [157] electrospun nanofibrous scaffolds, which are used for culturing different cells for various tissue engineering applications. Although there is no direct measurement to evaluate the efficiency of the collagen coating on the scaffolds, cell response, i.e., the promotion of specific biological activities, indirectly demonstrates the presence of collagen coating on electrospun fibrous scaffolds.…”

Section: Nanofibrous Scaffolds Coated With Bioactive Moleculesmentioning

confidence: 99%

Electrospinning Technology for Nanofibrous Scaffolds in Tissue Engineering

Li¹,

Shanti²,

Tuan³

2003

Nanotechnologies for the Life Sciences

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The sections in this article are Introduction Nanofibrous Scaffolds Fabrication Methods for Nanofibrous Scaffolds Phase Separation Self‐assembly Electrospinning The Electrospinning Process History Setup Mechanism and Working Parameters Properties of Electrospun Nanofibrous Scaffolds Architecture Porosity Mechanical Properties Current Development of Electrospun Nanofibrous Scaffolds in Tissue Engineering Evidence Supporting the Use of Nanofibrous Scaffolds in Tissue Engineering Nanofibrous Scaffolds Enhance Adsorption of Cell Adhesion Molecules Nanofibrous Scaffolds Induce Favorable Cell– ECM Interaction Nanofibrous Scaffolds Maintain Cell Phenotype Nanofibrous Scaffolds Support Differentiation of Stem Cells Nanofibrous Scaffolds Promote in vivo ‐like 3 D Matrix Adhesion and Activate Cell Signaling Pathway Biomaterials Electrospun into Nanofibrous Scaffolds Natural Polymeric Nanofibrous Scaffolds Synthetic Polymeric Nanofibrous Scaffolds Composite Polymeric Nanofibrous Scaffolds Nanofibrous Scaffolds Coated with Bioactive Molecules Engineered Tissues using Electrospun Nanofibrous Scaffolds Skin Blood Vessel Cartilage Bone Muscle Ligament Nerve Current Challenges and Future Directions Conclusion

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Fabrication of collagen-coated biodegradable polymer nanofiber mesh and its potential for endothelial cells growth

Cited by 437 publications

References 23 publications

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Endothelial Cell Lining of PET Vascular Prostheses: Modification with Degradable Polyester-based Copolymers and Adhesive Protein Multi-layers

Electrospinning Technology for Nanofibrous Scaffolds in Tissue Engineering

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