Fabrication of Coated-Collagen Electrospun PHBV Nanofiber Film by Plasma Method and Its Cellular Study

Abstract

Background. Tissue engineering is defined as the designing and engineering of structures to rebuild and repair a body damaged tissue. Scaffolding Poly Hydroxy Butyrate Valerate (PHBV) has shown good biocompatibility and biodegradable properties. Nanofibers have improved the performance of biomaterials, and could be considered effective. One of the important methods for designing nanofiber scaffold is the electrospinnig method. In this study, PHBV nanofibers were well designed and then, modified with the immobilized collagen via the plasma method. The samples were evaluated by ATR-FTIR, SEM, contact angle, and, finally, cell culture.Results. ATR-FTIR structural analysis showed the presence of collagen on the nanofiber surfaces. The SEM images showed the size average of nanofibers as to be about 280 nm; that increased with a collagen coating up to 300 nm. Contact angle analysis showed 67 degree for uncoated nanofibers and 56 degree for coated nanofibers. Cellular investigations (USS cells) showed better adhesion and cell growth and proliferation of coated samples than uncoated samples.Conclusions. In this work, the PHBV nanofibers with a size average about 280 nm were designed. Nanofibers were successfully coated with collagen via the plasma methods. These collagen-coated nanofibers could be used well for tissue engineering.

Surface Modification of PHBV Nanofibrous Mat by Laminin Protein and Its Cellular Study

“…To achieve positive cellbiomaterial interaction, functional groups or biomacromolecules have been immobilized onto PHAs for direct tuning of surface without altering their bulk properties [1][2][3][4][5][6][7][8]. Electrospinning has been rapidly developed into a technique to prepare nanofibers with the diameter ranging from tens of nanometers to several microns [9][10][11][12][13][14][15][16][17][18][19][20][21][22]. Controlling surface properties is very important for the high performance of adhesion.…”

Behavioral evaluation of regenerated rat sciatic nerve by a nanofibrous PHBV conduit filled with Schwann cells as artificial nerve graft

“…Nanofi bers have improved the performance of biomaterials. Electro spinning is one of the most important methods for the fabrication of nanofi brous scaffolds (Majdi et al, 2011;Biazar et al, 2010b;Rezaei-Tavirani et al, 2011;Ai et al, 2011;Heidari et al, in press;Biazar et al, 2013;Montazeri et al, 2011). Neuroscience researchers used different methods for detection of functional recovery in regenerated nerve.…”

Cellular Response of Limbal Stem Cells on PHBV/Gelatin Nanofibrous Scaffold for Ocular Epithelial Regeneration

“…PHAs are generally biodegradable, with good biocompatibility, making them attractive as tissue engineering biomaterials. Electrospinning is one of the most important methods for fabrication of nanofibrous scaffolds [18][19][20][21][22][23][24][25][26]. Electrospinning processes can fabricate nanofibers with a diameter ranging from a few tens to hundreds of nanometers and with a defined porosity.…”