Unrestricted somatic stem cells (USSCs) loaded in nanofibrous PHBV scaffold can be used for skin regeneration when grafted into full-thickness skin defects of rats. Nanofibrous PHBV scaffolds were designed using electrospinning method and then, modified with the immobilized collagen via the plasma method. Afterward, the scaffolds were evaluated using scanning electron microscopy, physical and mechanical assays. In this study; nanofibrous PHBV scaffolds loaded with and without USSCs were grafted into the skin defects. The wounds were subsequently investigated at 21 days after grafting. Results of mechanical and physical analyses showed good resilience and compliance to movement as a skin graft. In animal models; all study groups excluding the control group exhibited the most pronounced effect on wound closure, with the statistically significant improvement in wound healing being seen on post-operative Day 21. Histological and immunostaining examinations of healed wounds from all groups, especially the groups treated with stem cells, showed a thin epidermis plus recovered skin appendages in the dermal layer. Thus, the graft of collagen-coated nanofibrous PHBV scaffold loaded with USSC showed better results during the healing process of skin defects in rat model.
The layer-by-layer (LbL) assembly technique has shown excellent potential for tissue engineering applications. The technique is mainly based on electrostatic attraction and involves the sequential adsorption of oppositely charged electrolyte...
In this study, poly(lactic‐co‐glycolic acid) (PLGA)–gelatin scaffolds were fabricated using the freeze‐casting technique. Polydopamine (PDA) coating was applied on the surface of scaffolds to enhance the hydrophilicity, bioactivity, and cellular behavior of the composite constructs. Further, the synergistic effect of PDA coating and lamellar microstructure of scaffolds was evaluated on the promotion of properties. Based on morphological observations, freeze‐casting constructs showed lamellar pore channels while the uniformity and pore size were slightly affected by deposition of PDA. The hydrophilicity and swelling capacity of the scaffolds were assessed using contact angle measurement and phosphate buffered saline absorption ratio. The results indicated a significant increment in water–matrix interactions following surface modification. The evaluation of the biodegradation ratio revealed the higher degree of degradation in PDA‐coated samples owing to the presence of hydrophilic functional groups in the chemical structure of PDA. On the other hand, the bioactivity potential of PDA in the simulated body fluid solution confirmed the possibility of using coated constructs as a bone reconstructive substitute. The improvement of cellular attachment and filopodia formation in PDA‐contained matrixes was the other benefit of the coating process. Furthermore, cellular proliferation and ALP activity were enhanced after PDA coating. The suggested PDA‐coated PLGA–gelatin scaffolds can be applied in bone tissue regeneration.
Nanofibers have improved the performance of biomaterials, and can be considered effective. In this study, PHBV nanofibers were designed and then modified and crosslinked by oxygen plasma and laminin. The samples were evaluated by attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscope (SEM), contact angle, mechanical analyses, and cell culture. ATR-FTIR structural analysis showed the presence of functional groups on the nanofibrous surfaces. The SEM images showed the average size of nanofibers to be about 100 nm for the samples. The 70 difference was obtained in the contact angle analysis, obtained for the laminin-crosslinked nanofibrous mat than the unmodified nanofibrous mat. Cellular investigation showed better adhesion and cell growth and proliferation of laminin-crosslinked nanofibrous samples than other samples. The bioavailability of PHBV fibers with covalently attached laminin was found to be identical to that of PHBV fibers with physically adsorbed laminin, indicating that covalent attachment of protein is a suitable method for enhancing the biocompatibility of tissue engineering scaffolds.
Tissue engineering is defined as the designing and engineering of structures to rebuild and repair a body damaged tissue. Scaffolding Poly Hydroxy Butyrate Valrate (PHBV) has shown good biocompatibility and biodegradable properties. Nanofibers have improved the performance of biomaterials, and could be considered effective. One of the important methods for designing nanofiber scaffold is the electrospinnig method. In this study, PHBV nanofibers were well designed; then, modified with the immobilized gelatin via the chemically method. The samples were evaluated by ATR-FTIR, SEM, and finally, myocardiocyte culture. ATR-FTIR structural analysis showed the presence of gelatin on the nanofiber surfaces. The SEM images showed the size average of nanofibers as to be about 280 nm; that increased with a gelatin coating up to 500 nm. Cellular investigations (myocardiocyte) showed better adhesion and cell growth and proliferation of coated samples than uncoated samples. In this work, the PHBV nanofibers with a size average about 280 nm were designed. Nanofibers were successfully coated with gelatin via the chemically methods. These gelatin-coated nanofibers could be used well for heart tissue engineering.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.