Fabrication of chitosan-polyvinyl alcohol and silk electrospun fiber seeded with differentiated keratinocyte for skin tissue regeneration in animal wound model

Abstract

Hybrid fibrous mat containing cell interactive molecules offers the ability to deliver the cells and drugs in wound bed, which will help to achieve a high therapeutic treatment. In this study, a co-electrospun hybrid of polyvinyl alcohol (PVA), chitosan (Ch) and silk fibrous mat was developed and their wound healing potential by localizing bone marrow mesenchymal stem cells (MSCs)-derived keratinocytes on it was evaluated in vitro and in vivo. It was expected that fabricated hybrid construct could promote wound healing due to its structure, physical, biological specifications. The fabricated fibrous mats were characterized for their structural, mechanical and biochemical properties. The shape uniformity and pore size of fibers showed smooth and homogenous structures of them. Fourier transform infrared spectroscopy (FTIR) verified all typical absorption characteristics of Ch-PVA + Silk polymers as well as Ch-PVA or pure PVA substrates. The contact angle and wettability measurement of fibers showed that mats found moderate hydrophilicity by addition of Ch and silk substrates compared with PVA alone. The mechanical features of Ch-PVA + Silk fibrous mat increase significantly through co-electrospun process as well as hybridization of these synthetic and natural polymers. Higher degrees of cellular attachment and proliferation obtained on Ch-PVA + Silk fibers compared with PVA and Ch-PVA fibers. In terms of the capability of Ch-PVA + Silk fibers and MSC-derived keratinocytes, histological analysis and skin regeneration results showed this novel fibrous construct could be suggested as a skin substitute in the repair of injured skin and regenerative medicine applications.

Adipose Tissue-Derived Mesenchymal Stem Cells for Breast Tisssue Regeneration

“…Electrospinning is a commonly technique used for tissue engineering various scaffolds [106,107], however this is usually in applications where cancer has not been an issue, as scaffold ECM could encourage the growth of potentially malignant cells [108]. Electrospun scaffolds made from fibers can increase adipose tissue growth and adhesion more so than collagen, while also being shown to act as an immunosuppressant, when using polymers polydioxanone, polyglycolic acid, polylactic acid, and PCL [108,109].…”

Cetyl Trimethyl Ammonium Bromide Modified Montmorillonite‐Doped Tasar Silk Fibroin/Polyvinyl Alcohol Blend 3D Nanowebs for Tissue Engineering Applications

“…It was evident from the previous literature that blending of natural polymers such as silk fibroin with other artificial polymers has enhanced their cytocompatibility and cell adhesion property. [63][64][65][66] This could be attributed to the presence of cell adhesion moieties such as RGD sequence present in silk fibroin. Further, as observed in AFM studies, reinforcement of CTAB-MT has enhanced the surface roughness of the mats which might have aided in the cell adhesion and spreading.…”

Human Hair Keratin Composite Scaffold: Characterisation and Biocompatibility Study on NIH 3T3 Fibroblast Cells

“…However, since wool keratin is obtained from animal origins, it may lead to the possibility of immunogenicity. PVA has high hydrophilicity, chemical, and mechanical resistance [ 9 ]. Therefore a polymer that is a combination of both synthetic and biological materials can be used to develop a scaffold with improved biological functionality and mechanical properties with controlled degradation and biocompatibility [ 10 ].…”