Fabrication of Chitin/Poly(butylene succinate)/Chondroitin Sulfate Nanoparticles Ternary Composite Hydrogel Scaffold for Skin Tissue Engineering

Abstract

Skin loss is one of the oldest and still not totally resolved problems in the medical field. Since spontaneous healing of the dermal defects would not occur, the regeneration of full thickness of skin requires skin substitutes. Tissue engineering constructs would provide a three dimensional matrix for the reconstruction of skin tissue and the repair of damage. The aim of the present work is to develop a chitin based scaffold, by blending it with poly(butylene succinate) (PBS), an aliphatic, biodegradable and biocompatible synthetic polymer with excellent mechanical properties. The presence of chondroitin sulfate nanoparticles (CSnp) in the scaffold would favor cell adhesion. A chitin/PBS/CSnp composite hydrogel scaffold was developed and characterized by SEM (Scanning Electron Microscope), FTIR (Fourier Transform Infrared Spectroscopy), and swelling ratio of scaffolds were analyzed. The scaffolds were evaluated for the suitability for skin tissue engineering application by cytotoxicity, cell attachment, and cell proliferation studies using human dermal fibroblasts (HDF). The cytotoxicity and cell proliferation studies using HDF confirm the suitability of the scaffold for skin regeneration. In short, these results show OPEN ACCESSPolymers 2014, 6 2975 promising applicability of the developed chitin/PBS/CSnps ternary composite hydrogel scaffolds for skin tissue regeneration.

Effects of magnesium silicate on the mechanical properties, biocompatibility, bioactivity, degradability, and osteogenesis of poly(butylene succinate)-based composite scaffolds for bone repair

“…The diffraction peaks at 19.31, 22.21, 25.91 and 291 could be found in C0, which is in accordance with the crystallographic phases of PBSu of (002), (110), (121) and (111), respectively. 22 A broad reflection at 2y = 15-351 was exhibited in the XRD pattern of m-MS, indicating the typical amorphous silicate structure of m-MS. The position of the diffraction peaks for PBSu in C20 and C40 was not altered after the introduction of m-MS as compared with C0.…”

Regeneración Del Órgano Cutáneo Mediante Ingeniería De Tejidos

“…Estudios de la regeneración de tejidos de la piel, utilizan un andamio de hidrogel compuesto de quitina mezclado con polibutilsuccinato y nanopartículas de sulfato de condroitina [38]. La quitina es un apósito ideal que imita la matriz extracelular nativa ya que humedece la interfaz de la herida, es permeable al oxígeno, actúa como una barrera para los microorganismos, puede eliminar el exceso de secreciones y además posee propiedades como la compatibilidad inmunológica y flexibilidad [39].…”

“…Esto aumentaría la adsorción de proteínas del andamio y, por lo tanto, aumentaría la unión celular. Este andamio se caracteriza por SEM (microscopio electrónico de barrido), FTIR (espectroscopia de infrarrojo por transformada de Fourier) y se analizó la relación de hinchamiento de los andamios [38]. Los andamios se evaluaron en cuanto a la idoneidad para la aplicación de ingeniería tisular de la piel mediante estudios de citotoxicidad, unión y proliferación celular utilizando fibroblastos dérmicos humanos (HDF).…”

“…Los andamios se evaluaron en cuanto a la idoneidad para la aplicación de ingeniería tisular de la piel mediante estudios de citotoxicidad, unión y proliferación celular utilizando fibroblastos dérmicos humanos (HDF). Los estudios de citotoxicidad y proliferación celular que utilizan HDF confirman la idoneidad del andamio para la regeneración de la piel [38].…”

Drug Micro-Carriers Based on Polymers and Their Sterilization

“…Several studies have addressed the evaluation of the cytotoxicity of PBS, both in cells and in animals, which is key in their application in tissue replacement or as drug carriers [119][120][121][122]. Some authors have studied the cytotoxicity of PBS scaffolds in osteoblasts, fibroblasts and femoral bone of white rabbits, demonstrating the potential of PBS in tissue engineering applications [123][124][125].…”