Fabrication of cardiac patch by using electrospun collagen fibers

Abstract

International audienceSynergy between micro-nanotechnology and regenerative medicine can lead to new tools for health improvement. In this study, we investigate the efficacy of electrospun scaffolds-fabricated using clinically approved collagen – as supports for cardiomyoblast culture. The scaffolds were prepared using non-toxic solvents and crosslinking agents and characterized by scanning electron microscopy and contact angle measurements. Among different types of collagen samples, we found that atelocollagen can produce better quality of electrospun fibers than acid and basic fibrous collagen. Our results also show that the cell culture performance can be improved by adjusting the crosslinking conditions. Typically, increasing the concentration of citric acid of the cross-link agents from 5% to 10% w/w and the post-crosslink baking time from 1.5 to 2.5 h led to significant increases of the cellular colonization of the scaffold, showing three-dimensional growth of cardiac cells due to the specific morphology of the fibrous scaffolds. Finally, in vivo tests of the biocompatibility of the fabricated scaffolds have been done using a mouse model of dilated cardiomyopathy. As expected, the biocompatibility of the scaffold was found excellent and no visible inflammation was observed after the implantation up to two weeks. However, 5% citric acid electrospun collagen scaffolds was less resistant in vivo, proving again the importance of the processing parameter optimization of the electrospun scaffolds

Blood compatibility and physicochemical assessment of novel nanocomposite comprising polyurethane and dietary carotino oil for cardiac tissue engineering applications

“…The nanofibers obtained from electrospinning technique have diameters in the range 5–500 nm with small pore size and a high surface area, which possessed high impact on biomedical applications such as scaffoldings for damaged blood vessel replacement and cardiac patches . Researchers utilized electrospun polymeric scaffolds from polycaprolactone (PCL) and poly(L‐lactic acid) for the formation of functional cardiac cells layer . In another research, a functional composite made from PLGA/gelatin using electrospinning was fabricated .…”

Nanofibrous clinical-grade collagen scaffolds seeded with human cardiomyocytes induces cardiac remodeling in dilated cardiomyopathy

“…Electrospinning is an established nanotechnology technique for fabricating nanofibers by electrically charging a polymeric solution and has been extensively used for the fabrication of biomaterial scaffolds for tissue engineering (22)(23)(24). In a recent work, we have demonstrated the feasibility of obtaining electrospun collagen scaffolds with biologically-compatible solvents and cross-linking agents (25). In the present study, we leveraged these previous data to generate nanofibrous collagen scaffolds seeded with hiPS-CM and epicardially delivered these scaffolds in a mouse model of dilated cardiomyopathy based on the cardiac-specific and tamoxifen-inducible invalidation of serum response factor (SRF).…”

Heart on a chip: Micro-nanofabrication and microfluidics steering the future of cardiac tissue engineering

“…Electrospinning is a technique that is widely used for the fabrication of nanofibers, using polymeric (bio)materials or (bio)polymer based composites [13,90,91]. Currently it is the technique that is most widely used in industrial applications due to its ability to upscale, with many systems being developed in the last few years [92].…”

“…Schematic representations of the method, followed by advantages and disadvantages for their use in cardiac tissue engineering. Figures (reprinted with permission) are modified from the following: optical lithography from [21], soft lithography from [34], laser ablation from [64], plasma modification from [114], and electrospinning from [90].…”