Fabrication of Bacterial Cellulose Nanofibers/Soy Protein Isolate Colloidal Particles for the Stabilization of High Internal Phase Pickering Emulsions by Anti-solvent Precipitation and Their Application in the Delivery of Curcumin

Abstract

In this study, the anti-solvent precipitation and a simple complex method were applied for the preparation of bacterial cellulose nanofiber/soy protein isolate (BCNs/SPI) colloidal particles. Fourier transform IR (FT-IR) showed that hydrogen bonds generated in BCNs/SPI colloidal particles via the anti-solvent precipitation were stronger than those generated in BCNs/SPI colloidal particles self-assembled by a simple complex method. Meanwhile, the crystallinity, thermal stability, and contact angle of BCNs/SPI colloidal particles via the anti-solvent precipitation show an improvement in comparison with those of BCNs/SPI colloidal particles via a simple complex method. BCNs/SPI colloidal particles via the anti-solvent precipitation showed enhanced gel viscoelasticity, which was confirmed by dynamic oscillatory measurements. Furthermore, high internal phase Pickering emulsions (HIPEs) were additionally stable due to their stabilization by BCNs/SPI colloidal particles via the anti-solvent precipitation. Since then, HIPEs stabilized by BCNs/SPI colloidal particles via the anti-solvent precipitation were used for the delivery of curcumin. The curcumin-loaded HIPEs showed a good encapsulation efficiency and high 2,2-diphenyl-1-picrylhydrazyl (DPPH) removal efficiency. Additionally, the bioaccessibility of curcumin was significantly increased to 30.54% after the encapsulation using the prepared HIPEs. Therefore, it can be concluded that the anti-solvent precipitation is an effective way to assemble the polysaccharide/protein complex particles for the stabilization of HIPEs, and the prepared stable HIPEs showed a potential application in the delivery of curcumin.

Polysaccharide-Zein Composite Nanoparticles for Enhancing Cellular Uptake and Oral Bioavailability of Curcumin: Characterization, Anti-colorectal Cancer Effect, and Pharmacokinetics

“…In humans, oral administration of CUR at a dose of 2 g resulted in undetectable or extremely low serum levels ( 6 ). An array of carriers has been proposed to help overcome one or more of these shortcomings, and thus improve the efficacy of CUR in various disease models ( 7 , 8 ).…”

Pickering Emulsions Stabilized by Tea Water-Insoluble Protein Nanoparticles From Tea Residues: Responsiveness to Ionic Strength

“…The flocculation degree of divalent and trivalent metal ions is more than approximately 10–100 times that of univalent metal ions at the same ionic strength ( 47 ). Within a certain range, improving ionic strength can increase the hydrophobicity of particles to promote the adsorption of particles at the oil and water interface ( 48 ). An appropriate amount of ionic strength can also reduce the electrostatic repulsion between particles, which is conducive to the accumulation of particles at the surface of emulsion droplets to form a layer ( 44 ).…”

Research Progress of Food-Grade High Internal Phase Pickering Emulsions and Their Application in 3D Printing

“…Self-assembled composite particles are significantly affected by the type of particles, concentration, dissolution temperature, pH, ionic strength, and the self-assembly methods [ 51 , 52 ]. Shen et al [ 53 ] used the anti-solvent method with simple complexation to prepare protein–polysaccharide composite particles. Additionally, it was found that the particles prepared by the anti-solvent method had better protein conformation, surface activity, and gel strength, and the HIPPE stabilized with the anti-solvent composite particles had good droplet size, crystallinity, and thermal stability.…”

“…In the process of emulsion preparation, solid particles are wetted by both oil and water phases; the wettability of the particles is an important property to evaluate the emulsification ability of solid particles for HIPPE as well as the stability of the emulsion [ 53 ]. The wettability of the particles reflects their degree of hydrophilicity and hydrophobicity; only solid particles with dual wettability can be used as stabilizers for HIPPE.…”