Fabrication of anti-protein-fouling poly(ethylene glycol) microfluidic chip electrophoresis by sandwich photolithography

Cong, Hailin; Xu, Xiaodan; Yu, Bing; Liu, Huwei; Yuan, Hua

doi:10.1063/1.4959239

Cited by 12 publications

(8 citation statements)

References 39 publications

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“…Such devices are essentially of two-dimensional nature, and can be fabricated in a time span that is, in most cases, an order of magnitude shorter than the times required for conventional three-dimensional closed-channel microfluidic systems, which typically are fabricated using complex and costly fabrication procedures. Moreover, closed-channel microfluidic systems are associated with several practical difficulties, such as channel clogging by bubbles and fouling by debris . Nonspecific interactions of the analyte/reagent with the walls of the channel also cannot be avoided in some cases, which leads to false analysis outcomes.…”

Section: Introductionmentioning

confidence: 99%

Scaling Laws in Directional Spreading of Droplets on Wettability-Confined Diverging Tracks

et al. 2018

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Spontaneous pumpless transport of droplets on wettability-confined tracks is important for various applications, such as rapid transport and mixing of fluid droplets, enhanced dropwise condensation, biomedical devices, and so forth. Recent studies have shown that on an open surface, a superhydrophilic track of diverging width, laid on a superhydrophobic background, facilitates the transport of water from the narrower end to the wider end at unprecedented rates (up to 40 cm/s) without external actuation. The spreading behavior on such surfaces, however, has only been characterized for water. Keeping in mind that such designs play a key role for a diverse range of applications, such as handling organic liquids and in point-of-care devices, the importance of characterizing the spreading behavior of viscous liquids on such surfaces cannot be overemphasized. In the present work, the spreading behavior on the aforementioned wettability-patterned diverging tracks was observed for fluids of different viscosities. Two dimensionless variables were identified, and a comprehensive relationship was obtained. Three distinct temporal regimes of droplet spreading were established: I), a Washburn-type slow spreading, II) a much faster Laplace pressure-driven spreading, and III), a sluggish density-augmented Tanner-type film spreading. The results offer design guidance for tracks that can pumplessly manage fluids of various viscosities and surface tensions.

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Section: Introductionmentioning

confidence: 99%

Scaling Laws in Directional Spreading of Droplets on Wettability-Confined Diverging Tracks

et al. 2018

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“…99 PEG is a commonly used polymer for coating PDMS and PMMA microchannels to prevent protein adsorption, and in this work it is employed as a material for the microchannel fabrication. The authors state that a sandwich photolithography approach simplifies the fabrication process in comparison to PDMS microchips, however it is still a multi-step process.…”

Section: Separation Modes and Conditionsmentioning

confidence: 99%

Recent advances in protein analysis by capillary and microchip electrophoresis

Dawod

Arvin

Kennedy

2017

Analyst

120

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This review article describes the significant recent advances in analysis of proteins by capillary and microchip electrophoresis during the period mid 2014 to early 2017. The review highlights the progressions, new methodologies, innovative instrumental modifications, and challenges for efficient protein analysis in human specimens, animal tissues, and plant samples. Protein analysis fields covered in this review include analysis of native, reduced, and denatured proteins in addition to Western blotting, protein therapeutics and proteomics.

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“…22,23 Cong et al demonstrated the addition of PEG to a microfluidic chip through sandwich photolithography. 24 Moreover, PEG has served as an additive agent for improving the sensitivity of blood typing in the manual test tube method. 25,26 By means of improving the detection level for irregular antibodies as well as reducing the errors emanating from nonspecific bindings, PEG exhibited a higher sensitivity for blood typing.…”

Section: Introductionmentioning

confidence: 99%

Blood-typing and irregular antibody screening through multi-channel microfluidic discs with surface antifouling modification

Chen

Chang

et al. 2019

Biomicrofluidics

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A novel surface modification technology for microfluidic disks was developed for multichannel blood-typing detection and irregular antibody screening. The antifouling material, poly(ethylene glycol) methacrylate (PEGMA), was used to modify the surface of the microfluidic disk for improving its hydrophilicity and blood compatibility. With the modification of PEGMA, the hydrophilicity was sufficiently improved with a 44.5% reduction of water contact angle. The modified microfluidic disk also showed good biocompatibility with a reduction of hemolytic index (from 3.4% to 1.2%) and platelet adhesion (from 4.6 × 10 4 /cm 2 to 1.9 × 10 4 /cm 2). Furthermore, the PEGMA modification technique conducted on the microfluidic disk achieved successful adjustment of burst frequency for each chamber in the microchannel, allowing a sequential addiction of reagents in the test protocol of manual polybrene (MP) blood typing. Clinical studies showed that the proposed MP microfluidic disk method not only performed at extremely high consistency with the traditional tube method in the identification of ABO/RhD blood types, but also accomplished an effective screening method for detecting irregular antibodies. In conclusion, this study demonstrated that the easily massproduced MP microfluidic disk exhibited good blood-typing sensitivity and was suitable for clinical applications.

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Fabrication of anti-protein-fouling poly(ethylene glycol) microfluidic chip electrophoresis by sandwich photolithography

Cited by 12 publications

References 39 publications

Scaling Laws in Directional Spreading of Droplets on Wettability-Confined Diverging Tracks

Scaling Laws in Directional Spreading of Droplets on Wettability-Confined Diverging Tracks

Recent advances in protein analysis by capillary and microchip electrophoresis

Blood-typing and irregular antibody screening through multi-channel microfluidic discs with surface antifouling modification

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