Fabrication of a quinone containing layer on gold nanoparticles directed to a label-free and reagentless electrochemical miRNA sensor

Abstract

In this work, we report a simple electrochemical method to fabricate a label-free and reagentless electrochemical sensor for microRNA detection based on self-assembly of a multifunctional layer on gold nanoparticles-modified glassy carbon electrodes (AuNPs/GCEs).

Metal-Organic Framework MIL-53(Fe): Synthesis, Electrochemical Characterization, and Application in Development of a Novel and Sensitive Electrochemical Sensor for Detection of Cadmium Ions in Aqueous Solutions

“…The important characteristics of the sensor include sensitivity, stability, selectivity, response time, cost, and reusability [9]. To improve the sensitivity of electrochemical sensors, advanced materials have been applied to modified electrodes, including nanostructured conducting polymers [12,15], metal and metal oxide nanoparticles [16][17][18], nanostructured carbon materials (such as porous carbon, carbon nanotube, and graphene/graphene oxide) [17][18][19], and metal-organic frameworks (MOFs) [6,9,[20][21][22][23][24]. Among them, MOF materials which are constructed by metal ions or clusters and organic ligands via coordination bonds have unique properties including their structural diversity, flexible framework functionality, large metal cluster density, high stability, abundant adsorption sites, and a large surface area exceeding that of activated carbons and zeolites which are traditional porous materials [9,21,[23][24][25][26].…”

Redox-active benzoquinones as challenging “non-innocent” linkers to construct 2D frameworks and nanostructures with tunable physical properties

“…161 The direct and mediated electron transfer mechanisms were studied for the CNTs and CNTs-decorated with gold NPs, revealing a catalytic current density of 781 and 925 μA cm −2 , respectively. Gold NPs alone were coated with several benzoquinone-based capping agents, such as hydroquinone ( 28 , 29 ), 148,162 5-hydroxy-3-hexanedithiol-1,4-naphthoquinone ( 30 ), 163 theaflavine ( 31 ), 151 calix[7]hydroquinone ( 32 ), 149 or Ac-(3,4-dihydroxyphe-nylalanine) 4− methoxy-poly(ethylene glycol) amine ( 33 ). 150 The hydroquinone units are commonly employed as reducing agents to promote the growth of NPs or as an electrochemical sensor for microRNA detection, while the orthoquinones are employed as anti-cancer.…”

Recent Progress in Nanomaterials Modified Electrochemical Biosensors for the Detection of MicroRNA

“…The incorporation of nanomaterials in addition to signal amplification strategy significantly enhanced the sensitivity and detection limit of electrochemical miRNA biosensor, such as the modification of electrode surface with AuNPs/Ti 3 C 2 MXene, AuNPs, addition of Ag-PEI NPs as electroactive label and addition of nanoscale copper based metal organic framework assembled Pt NPs and horseradish peroxidase (Cu-NMOF@PtNPs/HRP) as catalytic nanoprobe for the detection of miR-155 [32,[74][75][76]. Similarly, AuNPs, cysteamine-capped AuNPs, amino-functionalized graphene quantum dots (GQDs), graphene oxide (GO), electrochemically-reduced graphene oxide/gold nanowires (ERGO/AuNRs), GO/AuNRs, black phosphorus nanosheets/thionine-doped copper-MOF (BPNSs/TH/Cu-MOF) and C 60 @PAMAM-MOF were applied to modify the electrode surface for sensitive detection of miR-103, miR-25, miR-34a, miR-137, miR-199a-5p, miR-3123, and miR-3675-3p [77][78][79][80][81][82][83][84][85]. Furthermore, nanomaterials also serve as electroactive label for enhancing the electrochemical signal generation.…”