Fabrication of a nonenzymatic cholesterol biosensor using carbon nanotubes from coconut oil

Abstract

A nonenzymatic cholesterol biosensor was fabricated at the surface of carbon nanotube electrode (CCNT), obtained from coconut oil. The voltammetric behavior of cholesterol at CCNT electrode was investigated by cyclic voltammetry and differential pulse voltammetry in the presence of 0.001 M H 2 SO 4 as electrolyte . The influence of the experimental parameters on the peak currents of cholesterol like pH, accumulation time, and scan rates were optimized. Under optimum conditions, the peak current was found to be linear in the cholesterol concentration range from 1 to 50 lM with a sensitivity of *15.31 ± 0.01 lA lM -1 cm -2 and response time of about 6 s. Some characteristic studies of the nonenzymatic biosensor, such as reproducibility, substrate specificity, and storage stability have also been studied.

Electrochemical detection of l-serine and l-phenylalanine at bamboo charcoal–carbon nanosphere electrode

“…As reported earlier [19], the CN electrode was designed like the commercially available standard electrode DS110CNT. It was fabricated on Teflon material containing three silver wires, where both working and counter electrodes were made of CN and third silver wire was acted as reference electrode.…”

“…In continuation of our earlier studies [15][16][17][18][19], we have now reported the fabrication of a new L-serine and L-phenylalanine biosensor based on carbon nanospheres (CN), which was obtained by pyrolysis of bamboo. Electrochemical studies have been carried out using cyclic voltammetry (CV), differential pulse voltammetry (DPV), square wave voltammetry (SWV) and linear sweep voltammetry (LSV) for the detection of L-serine and L-phenylalanine in phosphate buffer solution (PBS) at pH 6.8 and 7, respectively.…”

Voltammetric Determination of Dopamine Using Glassy Carbon Electrode Modified with ZnO/Al2O3 Nanocomposite

“…To have a selective and sensitive electrochemical signal for dopamine, the electrode modification is required. The application of modified electrodes in electrochemical method for the determination of drugs has been previously reported in the literature [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30]. Among various potential electrodes in the electrochemical determinations, glassy carbon electrodes (GCEs) have demonstrated the stability and resistance.…”

A Nanostructure Based Electrochemical Sensor for Square Wave Voltammetric Determination of L‐Cysteine in the Presence of High Concentration of Folic Acid

“…Generally,t he determination of Cys is carried out by various methods,i ncluding chromatography [2-4], electrophoresis [5],s pectrophotometry [6-9],a nd chemiluminescence [10].C ys can be studied also via electrochemical techniques [11][12][13][14].Combination of Cys and FA are effective against signs of aging and also Cys is effective on the FA conjugation [12].S o, simultaneous determination of thesec ompounds is very important in biological samples.T ot he best of our knowledge,o nly one published paper has been reported on the voltammetric determination of Cysi nt he presence of FA using modified electrodes [12],w hich is the focus of the present study.C ompared with previous report, the sensor usedi nt his study has an excellent dynamic range and limit of detection for the Cysa nalysis in the simultaneous determination of Cys and FA.Compared with otherk ind of sensors,e lectrochemical sensors have manya dvantages.F or example,t he electrodes can sense the materials whicha re present within the host without doinga ny damage to the host system [15][16][17][18][19][20][21][22][23][24].O nt he other hand, the active sensing material on the electrode should act as ac atalyst and catalyze the reaction of the biochemical or food electroactive compounds to obtain the output signals [25,26].T he selection and de-velopment of an active material in electrochemical studies is an important issue.T he actives ensingm aterials maybe of any kind as whichever acts as ac atalyst for sensing ap articular analyte or as et of analytes.T he recent improvement in the nanoscience has paved the way for large number of new materials and devices of desirable properties whichh ave useful functions for numerous electrochemical sensors and biosensor applications [27][28][29].In this research, we have used cyclic voltammetry, linear sweep voltammetry (LSV), chronoamperometry and SWV as voltammetric techniques for electrochemical determinationo fC ys on the DMBQ/ZnO/NPs/CPE at pH 5.0. l-Cys is built directly from l-methionin as an essential aminoacid making its abundance in the body acritical factor to the bodys supply of l-cysteine.…”

“…Compared with otherk ind of sensors,e lectrochemical sensors have manya dvantages.F or example,t he electrodes can sense the materials whicha re present within the host without doinga ny damage to the host system [15][16][17][18][19][20][21][22][23][24].O nt he other hand, the active sensing material on the electrode should act as ac atalyst and catalyze the reaction of the biochemical or food electroactive compounds to obtain the output signals [25,26].T he selection and de-velopment of an active material in electrochemical studies is an important issue.T he actives ensingm aterials maybe of any kind as whichever acts as ac atalyst for sensing ap articular analyte or as et of analytes.T he recent improvement in the nanoscience has paved the way for large number of new materials and devices of desirable properties whichh ave useful functions for numerous electrochemical sensors and biosensor applications [27][28][29].…”