Fabrication of a nanoparticle-containing 3D porous bone scaffold with proangiogenic and antibacterial properties

Paris, Juan L.; Lafuente-Gómez, Nuria; Cabañas, M.V.; Román, J.; Peña, J. L.; Vallet‐Regí, María

doi:10.1016/j.actbio.2019.01.013

Cited by 49 publications

(42 citation statements)

References 67 publications

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“…Developing a multifunctional scaffold, which combines excellent biocompatibility, osteoinductivity, and antibacterial ability, which is considered to be the next-generation orthopedic implants for hard tissue engineering applications 43,44 . Selenium, silver, and antibacterial drugs, such as cephalexin and chlorhexidine, were commonly used to impart antibacterial function to the composite scaffolds [45][46][47][48] . Nguyen et al investigated that selenium nanoparticles could inhibit Staphylococcus aureus, with low toxicity to mammalian cells 49 .…”

Section: Discussionmentioning

confidence: 99%

Nanostructured selenium-doped biphasic calcium phosphate with in situ incorporation of silver for antibacterial applications

Nie

Hou

Wang

et al. 2020

Sci Rep

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Selenium-doped nanostructure has been considered as an attractive approach to enhance the antibacterial activity of calcium phosphate (cap) materials in diverse medical applications. in this study, the selenium-doped biphasic calcium phosphate nanoparticles (SeB-NPs) were first synthesized. Then, silver was in situ incorporated into SeB-NPs to obtain nanostructured composite nanoparticles (Ag SeB-NPs). Both SeB-NPs and Ag SeB-NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV-Vis), X-ray photoelectron spectroscopy (XPS), and Raman spectra. The results confirmed that the SeO 3 2− was doped at the po 4 3− position and silver nanoparticles were deposited on the surface of SeB-NPs. Next, Transmission Electron Microscopy (TEM) analysis displayed that the prepared Ag SeB-NPs had a needlecluster-like morphology. CCK-8 analysis revealed SeB-NPs and Ag SeB-NPs had good cytocompatibility with osteoblasts. the antibacterial activity of the prepared Ag SeB-NPs was confirmed by using Gramnegative E. coli and Gram-positive S. aureus. The above results manifested the significance of the final Ag SeB-NPs for biomedical applications. Tissue engineering has been widely used in bone graft and dentistry due to a large number of patients require grafting resulting from congenital conditions, trauma, and tumor resection, and so on 1-3. Mineralized tissues, such as bone, tooth enamel, dentin, and cementum, are enriched with significant amounts of ionic substitutions, including sodium, potassium, and carbonate groups. These elements can affect the functionality of related tissues 4,5. Calcium phosphate (CaP) based nanoparticles, including hydroxyapatite (Ca 10 (PO 4) 6 (OH) 2 , HA), β-tricalcium phosphate (Ca 3 (PO 4) 2 , β-TCP), tetracalcium phosphate (Ca 4 (PO 4) 2 O; TTCP), biphasic calcium phosphate (BCP), and so forth, were well-known in implant surgery because of their excellent biocompatibility, biodegradability, and tunable physicochemical properties. Compared to HA or β-TCP, biphasic calcium phosphate (BCP) composed of HA and β-TCP, performed better mechanical properties, higher biological activity, and adjustable degradation rate 6-10. Bacteria attached to the implanted medical devices or scaffold can cause healthcare-associated infections, such as the formation of biofilm, which further results in local inflammation and infection. Therefore, the scaffold with excellent antibacterial properties provides an overwhelming advantage in the clinic stage 11,12. Many metal nanoparticles, such as silver (Ag), zinc oxide (ZnO), and titanium dioxide (TiO 2), performed strong antibacterial properties and low toxicity towards mammalian cells, some of these nanoparticles have been widely applied in a range of areas 13-20. Silver (Ag) is stable in the body fluids and can be used in antibacterial implants due to silver can easily binds to bacterial DNA and RNA, resulting in bacteria death 21. However, the antibacterial effect of nanoparticles was influenced...

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Section: Discussionmentioning

confidence: 99%

Nanostructured selenium-doped biphasic calcium phosphate with in situ incorporation of silver for antibacterial applications

Nie

Hou

Wang

et al. 2020

Sci Rep

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“…In addition, MSNs-loaded scaffolds allow the co-delivery of therapeutic compounds. In this sense, it is possible to load cephalexin within the mesopores and vascular endothelial growth factors in the scaffold structure to achieve bacteria elimination and bone reconstruction [193].…”

Section: Addressing Bone Infections With Mesoporous Silica Nanoparticlesmentioning

confidence: 99%

“…[187] MSNs-loaded scaffolds for the co-delivery of cephalexin and vascular endothelial growth factor; [193] Vancomycin-loaded silica-based mesoporous glasses contained in PLGA scaffolds; [191] Vancomycin-loaded MSNs contained in collagen gelatin scaffolds; [192] 5. Mesoporous Silica Nanoparticles for the Treatment of Osteoporosis…”

Section: Escherichia Colimentioning

confidence: 99%

Mesoporous Silica Nanoparticles for the Treatment of Complex Bone Diseases: Bone Cancer, Bone Infection and Osteoporosis

2020

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Bone diseases, such as bone cancer, bone infection and osteoporosis, constitute a major issue for modern societies as a consequence of their progressive ageing. Even though these pathologies can be currently treated in the clinic, some of those treatments present drawbacks that may lead to severe complications. For instance, chemotherapy lacks great tumor tissue selectivity, affecting healthy and diseased tissues. In addition, the inappropriate use of antimicrobials is leading to the appearance of drug-resistant bacteria and persistent biofilms, rendering current antibiotics useless. Furthermore, current antiosteoporotic treatments present many side effects as a consequence of their poor bioavailability and the need to use higher doses. In view of the existing evidence, the encapsulation and selective delivery to the diseased tissues of the different therapeutic compounds seem highly convenient. In this sense, silica-based mesoporous nanoparticles offer great loading capacity within their pores, the possibility of modifying the surface to target the particles to the malignant areas and great biocompatibility. This manuscript is intended to be a comprehensive review of the available literature on complex bone diseases treated with silica-based mesoporous nanoparticles—the further development of which and eventual translation into the clinic could bring significant benefits for our future society.

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“…In a more recent publication, the same authors report the modification of the described agarose/HA-composite by adding vascular endothelial growth factor (VEGF) and mesoporous silica nanoparticles loaded with the antibiotic cephalexin to improve vascularization and reduce infections during bone defect healing. Drug release studies, in vitro evaluation using MC3T3-E1 preosteoblastic cells and an ex ovo test for angiogenetic potential revealed a controlled cephalexin release by using either free or encapsulated drug-amounts, positive results for cell adhesion and cytotoxicity as well as the capacity to induce the formation of new blood vessels due to the release of VEGF [122]. To resemble the bone mineral more properly in a synthetic bone graft, Kazimierczak et al prepared Zn-and Mg-doped HA and applied it with a foamed agarose/chitosan-matrix.…”

Section: Calcium Phosphate Hybridsmentioning

confidence: 99%

Polysaccharide-Based Systems for Targeted Stem Cell Differentiation and Bone Regeneration

et al. 2019

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Bone tissue engineering is an ever-changing, rapidly evolving, and highly interdisciplinary field of study, where scientists try to mimic natural bone structure as closely as possible in order to facilitate bone healing. New insights from cell biology, specifically from mesenchymal stem cell differentiation and signaling, lead to new approaches in bone regeneration. Novel scaffold and drug release materials based on polysaccharides gain increasing attention due to their wide availability and good biocompatibility to be used as hydrogels and/or hybrid components for drug release and tissue engineering. This article reviews the current state of the art, recent developments, and future perspectives in polysaccharide-based systems used for bone regeneration.

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Fabrication of a nanoparticle-containing 3D porous bone scaffold with proangiogenic and antibacterial properties

Abstract: Fabrication of a nanoparticle-containing 3D porous bone scaffold with proangiogenic and antibacterial properties,

Cited by 49 publications

References 67 publications

Nanostructured selenium-doped biphasic calcium phosphate with in situ incorporation of silver for antibacterial applications

Nanostructured selenium-doped biphasic calcium phosphate with in situ incorporation of silver for antibacterial applications

Mesoporous Silica Nanoparticles for the Treatment of Complex Bone Diseases: Bone Cancer, Bone Infection and Osteoporosis

Polysaccharide-Based Systems for Targeted Stem Cell Differentiation and Bone Regeneration

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