Fabrication of a Nanofibrous Scaffold for the In Vitro Culture of Cardiac Progenitor Cells for Myocardial Regeneration

Electrospun membranes of polycaprolactone are widely used for biomedical applications like wound dressings and tissue engineering scaffolds. It is important to sterilize this material using the most accepted method, the gamma irradiation. In this study, we have evaluated the sterilizability of electrospun polycaprolactone membranes with gamma radiation of varying doses. The irradiated materials were assessed for the changes in morphology, crystallinity, surface degradation, hydrophilicity, mechanical property, sterility and the cell proliferation. Our results demonstrate that electrospun polycaprolactone can be effectively sterilized by gamma irradiation, however a higher dose of radiation affect the 2 materials properties. The irradiated membranes showed improved hydrophilicity and fibroblast cell proliferation.

show abstract

“…[1][2][3][4][5]. Electrospinning of PCL has been reported by many workers for the past few decades [1,2,6,7].…”

Section: Introductionmentioning

confidence: 99%

Dose-Dependent Effects of Gamma Irradiation on the Materials Properties and Cell Proliferation of Electrospun Polycaprolactone Tissue Engineering Scaffolds

Augustine

Saha

Jayachandran

et al. 2015

International Journal of Polymeric Materials and Polymeric Biom

View full text Add to dashboard Cite

show abstract

“…It is well known that viscosity of the polymeric solution is a key parameter that has a significant effect on the fiber diameter because a highly viscoelastic solution requires a high level of tensile force and as a consequence a thick jet and finally a thick fiber is generated (Meechaisue et al, 2006). Furthermore, higher molecular weight of gelatin solution leads a higher viscoelasticity behavior and increases fiber diameter (Aghdam et al, 2014). (Chong et al, 2007) fabricated an electrospun scaffold of PCL and gelatin and observed the same changes in the fibers diameter.…”

Section: Morphological Characterization Of the Fibrous Scaffoldsmentioning

confidence: 82%

Reinforcement of a decellularized extracellular matrix-derived hydrogel using nanofibers for cardiac tissue engineering

Mashayekhan

Jahanshahi

Moghadam

2020

Int. J. Adv. Biol. Biomed. Res.

View full text Add to dashboard Cite

The role of heart disease in increasing worldwide death and the limited availability of organs for transplantation have encouraged multiple strategies to fabricate functional and implantable constructs. One of these strategies is to develop a biologically similar heart tissue scaffold, in which two types of fiber and hydrogel are commonly used. Toward this goal, taking advantage of both hydrogels properties and fibers features with excellent mechanical properties can be considered as a promising method. The purpose of this study was to develop a fiber/hydrogel composite of gelatin, poly-caprolactone (PCL), cardiac extracellular matrix (ECM), and chitosan. The fibrous scaffolds of PCL and gelatin were characterized by SEM, water drop contact angle test, FTIR, and mechanical tests. The results showed that the average diameter of nanofibers, hydrophilicity and mechanical properties of the fibrous scaffolds increased with increasing the gelatin content in the spinning solution. Furthermore, the results of mechanical tests indicated that by integrating fibers with gelatin to PCL mass ratio of 2 in the hydrogel of chitosan and ECM with a mass ratio equal to 1, we obtained a construct with similar mechanical properties to native heart tissue, which may be proposed as an appropriate scaffold for heart tissue engineering.

show abstract

“…Polycaprolactone (PCL) is a promising candidate for scaffolding because it is FDA approved, is low-cost, but also has soft and flexible characteristics [25,26]. For these reasons, Aghdam et al [27] used PCL and PGA to construct a synthetic nanofibrous scaffold to culture cardiac progenitor cells. They found cell viability was highest after six days of culture on the synthetic scaffold with 65:35 PCL:PGA weight ratio, and attribute this result to increased hydrophilic properties.…”

Section: Scaffoldsmentioning

confidence: 99%

Nanomaterials for Cardiac Myocyte Tissue Engineering

et al. 2016

View full text Add to dashboard Cite

Since their synthesizing introduction to the research community, nanomaterials have infiltrated almost every corner of science and engineering. Over the last decade, one such field has begun to look at using nanomaterials for beneficial applications in tissue engineering, specifically, cardiac tissue engineering. During a myocardial infarction, part of the cardiac muscle, or myocardium, is deprived of blood. Therefore, the lack of oxygen destroys cardiomyocytes, leaving dead tissue and possibly resulting in the development of arrhythmia, ventricular remodeling, and eventual heart failure. Scarred cardiac muscle results in heart failure for millions of heart attack survivors worldwide. Modern cardiac tissue engineering research has developed nanomaterial applications to combat heart failure, preserve normal heart tissue, and grow healthy myocardium around the infarcted area. This review will discuss the recent progress of nanomaterials for cardiovascular tissue engineering applications through three main nanomaterial approaches: scaffold designs, patches, and injectable materials.

show abstract

Fabrication of a Nanofibrous Scaffold for the In Vitro Culture of Cardiac Progenitor Cells for Myocardial Regeneration

Cited by 34 publications

References 53 publications

Dose-Dependent Effects of Gamma Irradiation on the Materials Properties and Cell Proliferation of Electrospun Polycaprolactone Tissue Engineering Scaffolds

Dose-Dependent Effects of Gamma Irradiation on the Materials Properties and Cell Proliferation of Electrospun Polycaprolactone Tissue Engineering Scaffolds

Reinforcement of a decellularized extracellular matrix-derived hydrogel using nanofibers for cardiac tissue engineering

Nanomaterials for Cardiac Myocyte Tissue Engineering

Contact Info

Product

Resources

About