Fabrication of 3D Printed Poly(lactic acid)/Polycaprolactone Scaffolds Using TGF-β1 for Promoting Bone Regeneration

Cheng, Cheng-Hsin; Shie, Ming‐You; Lai, Yi-Hui; Foo, Ning‐Ping; Lee, Mon-Juan; Yao, Chun-Hsu

doi:10.3390/polym13213731

Cited by 23 publications

(18 citation statements)

References 70 publications

(75 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Jeong et al have found that HA/ß-TCP composite scaffolds revealed superior cell proliferation than their control models in in vitro assays 28 June 2022 11:26:00 AM. Previous studies have shown that composite polymer-mineral, 3D-printed scaffolds do integrate well and promote bone regeneration in bone defects in vivo [1,9,42,46,47]. Using our approach of optimizing pore size and geometry, perhaps a further improvement in tissue regeneration can be achieved.…”

Section: Discussionmentioning

confidence: 94%

See 1 more Smart Citation

Optimizing Design Parameters of PLA 3D-Printed Scaffolds for Bone Defect Repair

et al. 2022

View full text Add to dashboard Cite

Current materials used to fill bone defects (ceramics, cement) either lack strength or do not induce bone repair. The use of biodegradable polymers such as PLA may promote patient healing by stimulating the production of new bone in parallel with a controlled degradation of the scaffold. This project aims to determine the design parameters maximising scaffold mechanical performance in such materials. Starting from a base cylindrical model of 10 mm height and of outer and inner diameters of 10 and 4 mm, respectively, 27 scaffolds were designed. Three design parameters were investigated: pore distribution (crosswise, lengthwise, and eccentric), pore shape (triangular, circular, and square), and pore size (surface area of 0.25 mm2, 0.5625 mm2, and 1 mm2). Using the finite element approach, a compressive displacement (0.05 mm/s up to 15% strain) was simulated on the models and the resulting scaffold stiffnesses (N/mm2) were compared. The models presenting good mechanical behaviors were further printed along two orientations: 0° (cylinder sitting on its base) and 90° (cylinder laying on its side). A total of n = 5 specimens were printed with PLA for each of the retained models and experimentally tested using a mechanical testing machine with the same compression parameters. Rigidity and yield strength were evaluated from the experimental curves. Both numerically and experimentally, the highest rigidity was found in the model with circular pore shape, crosswise pore distribution, small pore size (surface area of 0.25 mm2), and a 90° printing orientation. Its average rigidity reached 961 ± 32 MPa from the mechanical testing and 797 MPa from the simulation, with a yield strength of 42 ± 1.5 MPa. The same model with a printing orientation of 0° resulted in an average rigidity of 515 ± 7 MPa with a yield strength of 32 ± 1.6 MPa. Printing orientation and pore size were found to be the most influential design parameters on rigidity. The developed design methodology should accelerate the identification of effective scaffolds for future in vitro and in vivo studies.

show abstract

Section: Discussionmentioning

confidence: 94%

“…Growth factors have also been frequently used (BMP2, TGFß, etc.) in that matter with promising pre-clinical and clinical results [41,42]. They can improve osteogenesis and vascularization [21,43] as well as bioactivity [2].…”

Section: Discussionmentioning

confidence: 99%

Optimizing Design Parameters of PLA 3D-Printed Scaffolds for Bone Defect Repair

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The immersed MTDRs were incubated at room temperature. After the incubation, MTDRs were dried and filtered using filter paper [ 55 ]. The swelling ratio of the MTDRs was calculated using Equation (1) [ 25 , 56 ].…”

Section: Methodsmentioning

confidence: 99%

Anatomically and Biomechanically Relevant Monolithic Total Disc Replacement Made of 3D-Printed Thermoplastic Polyurethane

Nadhif

Ghiffary²,

Irsyad

et al. 2022

Polymers

View full text Add to dashboard Cite

Various implant treatments, including total disc replacements, have been tried to treat lumbar intervertebral disc (IVD) degeneration, which is claimed to be the main contributor of lower back pain. The treatments, however, come with peripheral issues. This study proposes a novel approach that complies with the anatomical features of IVD, the so-called monolithic total disc replacement (MTDR). As the name suggests, the MTDR is a one-part device that consists of lattice and rigid structures to mimic the nucleus pulposus and annulus fibrosus, respectively. The MTDR can be made of two types of thermoplastic polyurethane (TPU 87A and TPU 95A) and fabricated using a 3D printing approach: fused filament fabrication. The MTDR design involves two configurations—the full lattice (FLC) and anatomy-based (ABC) configurations. The MTDR is evaluated in terms of its physical, mechanical, and cytotoxicity properties. The physical characterization includes the geometrical evaluations, wettability measurements, degradability tests, and swelling tests. The mechanical characterization comprises compressive tests of the materials, an analytical approach using the Voigt model of composite, and a finite element analysis. The cytotoxicity assays include the direct assay using hemocytometry and the indirect assay using a tetrazolium-based colorimetric (MTS) assay. The geometrical evaluation shows that the fabrication results are tolerable, and the two materials have good wettability and low degradation rates. The mechanical characterization shows that the ABC-MTDR has more similar mechanical properties to an IVD than the FLC-MTDR. The cytotoxicity assays prove that the materials are non-cytotoxic, allowing cells to grow on the surfaces of the materials.

show abstract

“…Moreover, the osteogenic effect of PCL-based 3D scaffolds is significantly better than that of PLA-based 3D scaffolds. However, the hydrophobic nature of PCL often leads to a lower cell survival rate of the constructed bio-scaffolds [ 73 , 74 ]. PLA has good ductility and stiffness, processability, biocompatibility, and a fast biodegradation rate.…”

Section: Main Textmentioning

confidence: 99%

Emerging 3D bioprinting applications in plastic surgery

et al. 2023

Biomater Res

View full text Add to dashboard Cite

Plastic surgery is a discipline that uses surgical methods or tissue transplantation to repair, reconstruct and beautify the defects and deformities of human tissues and organs. Three-dimensional (3D) bioprinting has gained widespread attention because it enables fine customization of the implants in the patient's surgical area preoperatively while avoiding some of the adverse reactions and complications of traditional surgical approaches. In this paper, we review the recent research advances in the application of 3D bioprinting in plastic surgery. We first introduce the printing process and basic principles of 3D bioprinting technology, revealing the advantages and disadvantages of different bioprinting technologies. Then, we describe the currently available bioprinting materials, and dissect the rationale for special dynamic 3D bioprinting (4D bioprinting) that is achieved by varying the combination strategy of bioprinting materials. Later, we focus on the viable clinical applications and effects of 3D bioprinting in plastic surgery. Finally, we summarize and discuss the challenges and prospects for the application of 3D bioprinting in plastic surgery. We believe that this review can contribute to further development of 3D bioprinting in plastic surgery and provide lessons for related research. Graphical Abstract

show abstract

Fabrication of 3D Printed Poly(lactic acid)/Polycaprolactone Scaffolds Using TGF-β1 for Promoting Bone Regeneration

Cited by 23 publications

References 70 publications

Optimizing Design Parameters of PLA 3D-Printed Scaffolds for Bone Defect Repair

Optimizing Design Parameters of PLA 3D-Printed Scaffolds for Bone Defect Repair

Anatomically and Biomechanically Relevant Monolithic Total Disc Replacement Made of 3D-Printed Thermoplastic Polyurethane

Emerging 3D bioprinting applications in plastic surgery

Contact Info

Product

Resources

About