Fabrication, characterization, and in vitro study of zinc substituted hydroxyapatite/silk fibroin composite coatings on titanium for biomedical applications

Zhong, Zhenyu; Ma, Jun

doi:10.1177/0885328217723501

Cited by 25 publications

(11 citation statements)

References 47 publications

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“…Because of its biocompatibility, nontoxicity, nonirritation, good air permeability, oxygen permeability, and biodegradability, silk fibroin has been attracting interest in the study of drug carrier materials [29]. At present, silk fibroin can be fabricated into various forms, such as membranes [30], microspheres [31], hydrogels [32], porous scaffolds [33], and nanofibers [34]. Among them, the nanofiber membrane prepared by electrospinning technology has become a hotspot in carrier research because of its large specific surface area, high porosity, good cell compatibility, and similar structural characteristics to the natural extracellular matrix (ECM).…”

Section: Discussionmentioning

confidence: 99%

Dual-Drug-Loaded Silk Fibroin/PLGA Scaffolds for Potential Bone Regeneration Applications

Yao

Wang

et al. 2019

Journal of Nanomaterials

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Developing scaffold materials with excellent biocompatibility, mechanical properties, and controlled drug release properties is vital to tissue engineering. In this study, we fabricated silk fibroin (SF)/poly(lactide-co-glycolide) (PLGA) nanofiber scaffolds containing recombinant human bone morphogenetic protein 2 (rhBMP2) and dexamethasone (DXM) via coaxial electrospinning, which were used in in vitro bone formation with rat bone marrow mesenchymal stem cells (rBMSCs). An in vitro drug release study was adopted to evaluate the sustained release potential of the core-shell structured nanofibers. Furthermore, we detected the potential of the SF/PLGA nanofiber membrane in vitro. In vitro studies showed that rhBMP2 still remained active on the nanofiber membrane. In addition, the dual-drug-loaded nanofiber membrane showed an early burst release of DXM and late sustained release of rhBMP2. rhBMP2 and DXM exhibited strong osteogenic differentiation potential when they acted on rBMSCs. Therefore, the SF/PLGA nanofiber membrane loaded with rhBMP2 and DXM has great potential for the enhancement of bone regeneration.

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Section: Discussionmentioning

confidence: 99%

Dual-Drug-Loaded Silk Fibroin/PLGA Scaffolds for Potential Bone Regeneration Applications

Yao

Wang

et al. 2019

Journal of Nanomaterials

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“…According to Zhu et al [70], Zn biomaterial can support hMSC adhesion and proliferation and zinc ions can lead to enhanced regulation of genes, cell survival/growth and differentiation, extracellular matrix (ECM) mineralization, and osteogenesis. The stimulation of osteogenesis by ionic zinc has been supported by numerous studies [71][72][73][74][75][76][77][78][79]. In the case of human vascular cells, Zn 2+ at low concentrations can enhance cell viability, proliferation, adhesion, spreading, migration, and F-actin and vinculin expression, while decreasing cell adhesion strength.…”

Section: In Vitro Examinationmentioning

confidence: 90%

The Prospects of Zinc as a Structural Material for Biodegradable Implants—A Review Paper

Levy

Goldman

Aghion

2017

Metals

228

124

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Abstract:In the last decade, iron and magnesium, both pure and alloyed, have been extensively studied as potential biodegradable metals for medical applications. However, broad experience with these material systems has uncovered critical limitations in terms of their suitability for clinical applications. Recently, zinc and zinc-based alloys have been proposed as new additions to the list of degradable metals and as promising alternatives to magnesium and iron. The main byproduct of zinc metal corrosion, Zn 2+ , is highly regulated within physiological systems and plays a critical role in numerous fundamental cellular processes. Zn 2+ released from an implant may suppress harmful smooth muscle cells and restenosis in arteries, while stimulating beneficial osteogenesis in bone. An important limitation of pure zinc as a potential biodegradable structural support, however, lies in its low strength (σ UTS~3 0 MPa) and plasticity (ε < 0.25%) that are insufficient for most medical device applications. Developing high strength and ductility zinc with sufficient hardness, while retaining its biocompatibility, is one of the main goals of metallurgical engineering. This paper will review and compare the biocompatibility, corrosion behavior and mechanical properties of pure zinc, as well as currently researched zinc alloys.

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“…In some situations, the incorporation of Zn in HA powders had a toxic influence on cells (i.e., HepG2 cells—human hepatocytes [65,291]), as a consequence of Zn-HA particle sedimentation over cells. Excellent bioactivity of Zn-doped (2.4 at.%) HA was evidenced after only 3 days of soaking in SBF solution [292]. Zn-doped HA was confirmed as an effective antimicrobial agent against Gram-positive and Gram-negative bacteria frequently occurring at the implant site: e.g., S. aureus , Streptococcus mutans , Staphylococcus epidermidis , Enterobacter aerogenes , E. coli [65,274,288,289,291,293,294,295].…”

Section: Cation-substituted Hydroxyapatitesmentioning

confidence: 99%

Cationic Substitutions in Hydroxyapatite: Current Status of the Derived Biofunctional Effects and Their In Vitro Interrogation Methods

et al. 2018

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High-performance bioceramics are required for preventing failure and prolonging the life-time of bone grafting scaffolds and osseous implants. The proper identification and development of materials with extended functionalities addressing socio-economic needs and health problems constitute important and critical steps at the heart of clinical research. Recent findings in the realm of ion-substituted hydroxyapatite (HA) could pave the road towards significant developments in biomedicine, with an emphasis on a new generation of orthopaedic and dentistry applications, since such bioceramics are able to mimic the structural, compositional and mechanical properties of the bone mineral phase. In fact, the fascinating ability of the HA crystalline lattice to allow for the substitution of calcium ions with a plethora of cationic species has been widely explored in the recent period, with consequent modifications of its physical and chemical features, as well as its functional mechanical and in vitro and in vivo biological performance. A comprehensive inventory of the progresses achieved so far is both opportune and of paramount importance, in order to not only gather and summarize information, but to also allow fellow researchers to compare with ease and filter the best solutions for the cation substitution of HA-based materials and enable the development of multi-functional biomedical designs. The review surveys preparation and synthesis methods, pinpoints all the explored cation dopants, and discloses the full application range of substituted HA. Special attention is dedicated to the antimicrobial efficiency spectrum and cytotoxic trade-off concentration values for various cell lines, highlighting new prophylactic routes for the prevention of implant failure. Importantly, the current in vitro biological tests (widely employed to unveil the biological performance of HA-based materials), and their ability to mimic the in vivo biological interactions, are also critically assessed. Future perspectives are discussed, and a series of recommendations are underlined.

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Fabrication, characterization, and in vitro study of zinc substituted hydroxyapatite/silk fibroin composite coatings on titanium for biomedical applications

Cited by 25 publications

References 47 publications

Dual-Drug-Loaded Silk Fibroin/PLGA Scaffolds for Potential Bone Regeneration Applications

Dual-Drug-Loaded Silk Fibroin/PLGA Scaffolds for Potential Bone Regeneration Applications

The Prospects of Zinc as a Structural Material for Biodegradable Implants—A Review Paper

Cationic Substitutions in Hydroxyapatite: Current Status of the Derived Biofunctional Effects and Their In Vitro Interrogation Methods

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