Fabrication, characterization, and application of potentiometric immunosensor based on biocompatible and controllable three-dimensional porous chitosan membranes

Liang, Ru‐Ping; Peng, Hongzhen; Qiu, Jian‐Ding

doi:10.1016/j.jcis.2008.01.001

Cited by 46 publications

(28 citation statements)

References 37 publications

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“…Moreover, the use of CHIT has enabled the EPD fabrication of a new family of novel nanocomposite coatings containing other functional materials as filler. Composite CHIT -silica films were deposited for the fabrication of immunosensors (Liang et al 2008) and implants (Grandfield & Zhitomirsky 2008). Films were obtained with a three-dimensional porous structure, which possessed a high surface area, good mechanical stability and good hydrophilicity (Liang et al 2008).…”

Section: Chitosan-based Nanocompositesmentioning

confidence: 99%

“…Composite CHIT -silica films were deposited for the fabrication of immunosensors (Liang et al 2008) and implants (Grandfield & Zhitomirsky 2008). Films were obtained with a three-dimensional porous structure, which possessed a high surface area, good mechanical stability and good hydrophilicity (Liang et al 2008). Such films provide a biocompatible microenvironment for maintaining the bioactivity of immobilized proteins and enable protein loading.…”

Section: Chitosan-based Nanocompositesmentioning

confidence: 99%

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Electrophoretic deposition of biomaterials

Boccaccını

Keim

et al. 2010

J. R. Soc. Interface.

613

412

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Electrophoretic deposition (EPD) is attracting increasing attention as an effective technique for the processing of biomaterials, specifically bioactive coatings and biomedical nanostructures. The well-known advantages of EPD for the production of a wide range of microstructures and nanostructures as well as unique and complex material combinations are being exploited, starting from well-dispersed suspensions of biomaterials in particulate form (microsized and nanoscale particles, nanotubes, nanoplatelets). EPD of biological entities such as enzymes, bacteria and cells is also being investigated. The review presents a comprehensive summary and discussion of relevant recent work on EPD describing the specific application of the technique in the processing of several biomaterials, focusing on (i) conventional bioactive (inorganic) coatings, e.g. hydroxyapatite or bioactive glass coatings on orthopaedic implants, and (ii) biomedical nanostructures, including biopolymer-ceramic nanocomposites, carbon nanotube coatings, tissue engineering scaffolds, deposition of proteins and other biological entities for sensors and advanced functional coatings. It is the intention to inform the reader on how EPD has become an important tool in advanced biomaterials processing, as a convenient alternative to conventional methods, and to present the potential of the technique to manipulate and control the deposition of a range of nanomaterials of interest in the biomedical and biotechnology fields.

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Section: Chitosan-based Nanocompositesmentioning

confidence: 99%

Section: Chitosan-based Nanocompositesmentioning

confidence: 99%

Electrophoretic deposition of biomaterials

Boccaccını

Keim

et al. 2010

J. R. Soc. Interface.

613

412

View full text Add to dashboard Cite

show abstract

“…EIS can also be used to develop impedimetric sensors. For the major part of the studies in which the EIS technique was used to characterize each step of an electrode modification Fe(CN) 6 3-/4-redox couple was employed as a marker and the data were qualitatively analyzed (Xiulan et al 2011;Wang & Tan, 2007;Yuan et al, 2009;Wang et al, 2008;Liang et al, 2008). In the Nyquist plot a semicircle at high or middle frequencies followed by a straight line at lower frequencies were frequently observed.…”

Section: Electrochemical Impedance Spectroscopymentioning

confidence: 99%

Preparation and Characterization of Imunosensors for Disease Diagnosis

Ferreira¹,

Fugivara²,

Yamanaka³

et al. 2011

Biosensors for Health, Environment and Biosecurity

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“…This suggested that the HBsAb was successfully immobilized on the surface of CS-Fc/MWCNTs/Au NPs film. The unique three-dimensional structure could provide a friendly microenvironment for HBsAb incorporation and make the immobilized HBsAb easy to be accessed by HBsAg as compared to the planar film [36]. Moreover, the uniformly dispersed MWCNTs highly intimate contact with Fc and Au NPs acting as a conductive pathway, which increased the electron-transfer efficiency.…”

Section: Preparation and Characterization Of Different Membranesmentioning

confidence: 99%

“…As the solubility of CS is pH-dependent, when the pH exceeds the pK a of CS (about 6.3), it becomes insoluble [34][35][36], and thus CS-Fc hydrogel incorporated MWCNTs will co-deposit onto the electrode surface. The obtained film was denoted as CS-Fc/MWCNTs.…”

mentioning

confidence: 99%

A Label‐Free Amperometric Immunosensor Based on Redox‐Active Ferrocene‐Branched Chitosan/Multiwalled Carbon Nanotubes Conductive Composite and Gold Nanoparticles

et al. 2010

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A novel immunosensor has been developed by self-assembling Au NPs onto a ferrocene-branched chitosan/multiwalled carbon nanotubes (CS-Fc/MWCNTs) modified electrode for the sensitive determination of hepatitis B surface antigen (HBsAg). The formation of CS-Fc effectively avoids the leakage of Fc and retains its electrochemical activity. Incorporation of MWCNTs and Au NPs into CS-Fc further increases the electrochemical active Fc in the CS films and provides interactive sites for the immobilization of HBsAb. The morphologies and electrochemistry of the formed biofilm were investigated by using scanning electron microscopy and electrochemical techniques. The immunosensor exhibits a specific response to HBsAg in the range of 1.0-420 ng mL À1. Excellent analytical performance, fabrication reproducibility and operational stability of the proposed immunosensor indicated its promising application in clinical diagnostics.

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Fabrication, characterization, and application of potentiometric immunosensor based on biocompatible and controllable three-dimensional porous chitosan membranes

Cited by 46 publications

References 37 publications

Electrophoretic deposition of biomaterials

Electrophoretic deposition of biomaterials

Preparation and Characterization of Imunosensors for Disease Diagnosis

A Label‐Free Amperometric Immunosensor Based on Redox‐Active Ferrocene‐Branched Chitosan/Multiwalled Carbon Nanotubes Conductive Composite and Gold Nanoparticles

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