Fabrication, characterization and application of laccase–nylon 6,6/Fe3+ composite nanofibrous membrane for 3,3′-dimethoxybenzidine detoxification

Jasni, M. Jasmin Fathi; Sathishkumar, Palanivel; Yusoff, Abdull Rahim Mohd; Ameen, Fuad; Buang, Nor Aziah; Kadir, Mohammed Rafiq Abdul; Yusop, Zulkifli

doi:10.1007/s00449-016-1686-6

Cited by 43 publications

(18 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(b)). The raising of the optimum reactive temperature and pH could be explained by the fact that covalent binding between enzyme and NFM would change the enzyme's microenvironment and protect it from conformational denaturation . Moreover, the stability of laccase was significantly improved after laccase immobilization, as its reactivity remained almost unchanged over a temperature range of 30–60 °C and over a pH range of 5–8.…”

Section: Resultsmentioning

confidence: 99%

Enzymatic degradation of ginkgolic acid by laccase immobilized on novel electrospun nanofiber mat

et al. 2020

View full text Add to dashboard Cite

BACKGROUND Ginkgo biloba leaf extract contains many active ingredients that are beneficial for health. However, ginkgolic acid, one of the major components found in G. biloba extract, may cause serious allergic and toxic side effects. The purpose of this study is to immobilize the laccase system on the electrospun nylon fiber mat (NFM) to hydrolyze the ginkgolic acid in G. biloba leaf extract efficiently. RESULTS Novel electrospinning technology successfully produced high‐quality nanoscopic fiber mats made of a mixture of multi‐walled carbon nanotube and nylon 6,6. Laccase that was immobilized onto the NFM exhibited much higher efficiency in the catalyzation of 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) diammonium salt (ABTS) than nylon 6,6 pellets. After being immobilized onto the NFM, the pH and temperature stability of laccase were significantly improved. The NFM‐immobilized laccase could maintain more than 50% of its original activity even after 40 days of storage or 10 operational cycles. The kinetic parameters, including rate constant (K), the time (τ50) in which 50% of ginkgolic acid hydrolysis was reached, the time (τcomplete) required to achieve complete ginkgolic acid hydrolysis, Km and Vmax were determined, and were 0.07 ± 0.01 min−1, 8.97 ± 0.55 min, 45.45 ± 2.79 min, 0.51 ± 0.09 mM and 0.49 ± 0.03 mM min−1 mg−1, respectively. CONCLUSION The result successfully demonstrated the strong potential of using novel electrospun nanofiber mats as enzyme immobilization platforms, which could significantly enhance enzyme activity and stability. © 2020 Society of Chemical Industry

show abstract

Section: Resultsmentioning

confidence: 99%

Enzymatic degradation of ginkgolic acid by laccase immobilized on novel electrospun nanofiber mat

et al. 2020

View full text Add to dashboard Cite

show abstract

“…As reported, the resultant PA6/laccase conjugates are useful in industrial biocatalyst applications. Furthermore, a nanofibrous membrane for 3,3 -dimethoxybenzidine detoxification was developed by Jasni et al [22] on the basis of PA66/laccase/Fe 3+ conjugate produced by covalent bonding of the enzyme to the support by glutaraldehyde crosslinking. The immobilized enzyme displayed enhanced storage stability and a good potential for emerging pollutant detoxification.…”

Section: Introductionmentioning

confidence: 99%

Polymer-Assisted Biocatalysis: Polyamide 4 Microparticles as Promising Carriers of Enzymatic Function

et al. 2020

View full text Add to dashboard Cite

This study reports a new strategy for enzyme immobilization based on passive immobilization in neat and magnetically responsive polyamide 4 (PA4) highly porous particles. The microsized particulate supports were synthesized by low-temperature activated anionic ring-opening polymerization. The enzyme of choice was laccase from Trametes versicolor and was immobilized by either adsorption on prefabricated PA4 microparticles (PA4@iL) or by physical in situ entrapment during the PA4 synthesis (PA4@eL). The surface topography of all PA4 particulate supports and laccase conjugates, as well as their chemical and physical structure, were studied by microscopic, spectral, thermal, and synchrotron WAXS/SAXS methods. The laccase content and activity in each conjugate were determined by complementary spectral and enzyme activity measurements. PA4@eL samples displayed >93% enzyme retention after five incubation cycles in an aqueous medium, and the PA4@iL series retained ca. 60% of the laccase. The newly synthesized PA4-laccase complexes were successfully used in dyestuff decolorization aiming at potential applications in effluent remediation. All of them displayed excellent decolorization of positively charged dyestuffs reaching ~100% in 15 min. With negative dyes after 24 h the decolorization reached 55% for PA4@iL and 85% for PA4@eL. A second consecutive decolorization test revealed only a 5–10% decrease in effectiveness indicating the reusability potential of the laccase-PA4 conjugates.

show abstract

“…Figure 2 shows the FTIR spectra of the nylon 6,6 membrane. According to the obtained results, the nylon 6,6 has a medium band at peak 3298cm -1 that was attributed to the N-H stretch from amino groups [41]. Following this, a C-H stretching vibration due to alkanes group was observed with a medium peak at 2934cm -1 .…”

Section: Functional Groupsmentioning

confidence: 55%

“…This advanced, well-known treatment technology has become one of the most preferred options for water and wastewater treatments in food industries, chemical industries, and pharmaceutical industries [37][38][39]. Such popularity of membrane treatment is because of many advantages such as no addition of chemicals required, no secondary pollutants produced, low energy consumption, easy to handle, low operating and maintenance costs, easy to scale-up, high porous structure, and high recovery and reusability [40,41]. Most of the membranes are made from polymeric materials.…”

Section: Introductionmentioning

confidence: 99%

A Combination of Waste Biomass Activated Carbon and Nylon Nanofiber for Removal of Triclosan from Aqueous Solutions

2020

J. Environ. Treat. Tech.

View full text Add to dashboard Cite

Triclosan (TCS) is one of the biocide used as antibacterial and antifungal agent to kill and hinder the growth of bacteria and also it is used in many personal care and health care products. However, TCS can cause health and environmental problems such as environmental pollutions, acute toxicity, etc. The aim of this study is to investigate the removal of TCS from aqueous solution by combining the coconut pulp waste (Cocos nuciefera) activated carbon (AC) with nylon 6,6 membrane. To this end, first, the effects of physico-chemical characteristics of the membrane were studied. The nylon 6,6 membrane (14 wt.%] was prepared using electrospinning machine with injection rate at 0.4 mL/h, tip-to-collector distance at 15 cm, rotation speed at 1000 rpm, and applied voltage at 26 kV. The parameters studied for the membrane during the adsorption test were contact time, adsorbent dosage, agitation speed, initial TCS concentration, pH, and temperature of the TCS solution. The filtration test was done using flat sheet membrane test machine at pressure 1.0 bar. The characteristics of the membrane were analysed using the FESEM and FTIR tests. Based on the obtained results, the nylon 6,6 membrane can remove 90.2% of TCS within 5 minutes; the removal rate increased to 100% in less than 5 minutes after the membrane was combined with AC. This study proved that the combination of AC and nylon 6,6 membrane is able to maximize the TCS removal from water.

show abstract

Fabrication, characterization and application of laccase–nylon 6,6/Fe3+ composite nanofibrous membrane for 3,3′-dimethoxybenzidine detoxification

Cited by 43 publications

References 28 publications

Enzymatic degradation of ginkgolic acid by laccase immobilized on novel electrospun nanofiber mat

Enzymatic degradation of ginkgolic acid by laccase immobilized on novel electrospun nanofiber mat

Polymer-Assisted Biocatalysis: Polyamide 4 Microparticles as Promising Carriers of Enzymatic Function

A Combination of Waste Biomass Activated Carbon and Nylon Nanofiber for Removal of Triclosan from Aqueous Solutions

Contact Info

Product

Resources

About