Polymer-Assisted Biocatalysis: Polyamide 4 Microparticles as Promising Carriers of Enzymatic Function

Dencheva, Nadya Vasileva; Braz, Joana Filipa Barros; Scheibel, Dieter M.; Malfois, Marc; Denchev, Z.; Gitsov, Ivan

doi:10.3390/catal10070767

Cited by 14 publications

(19 citation statements)

References 48 publications

(53 reference statements)

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“…This is the first report on catechol transformations mediated by Trametes versicolor laccase immobilized on PA6 supports. It differs from the previous study on PA4-based laccase conjugates [43] in several crucial aspects. The PA6 microparticulate supports were synthesized by AAROP at 120ºC and atmospheric pressure, while for the PA4 supports temperatures as low as 40ºC and vacuum were needed for the polymerization to produce microparticles.…”

Section: Discussioncontrasting

confidence: 82%

“…Notably, the strong complex peak at 1014 cm −1 of the lyophilized laccase is not present in the adsorption-immobilized laccase conjugate. A similar effect was observed in laccase-immobilized PA4 MP [43].…”

Section: Ftir Spectroscopysupporting

confidence: 78%

“…This result can be considered quite good keeping in mind that the immobilized laccases display typically lower activities than the free ones due to limited mass transfer and obstruction of the Cu active centers [54]. The activities of the PA6@L samples compare favorably to the results obtained with similar systems based on PA4 [43], in which the immobilized laccase was 1.7-2.6 times less active than the free enzyme. This effect can be attributed to the much larger pores on the surface of the PA6 MP (350-700 nm versus 100-150 nm in the PA4 MP supports), causing less mass-transfer limitations.…”

Section: Enzymatic Activity Of Pa6@l Conjugatessupporting

confidence: 65%

“…Consequently, the laccase adsorption on PA6Fe support should occur almost in monolayers; on PA6-in bilayers, while on PA6Fe3O4 and PA6FeP the number of layers would vary between 1.3 and 1.7, respectively. A comparison can be made with laccase immobilized PA4 MP in our previous study [43] where deposition of laccase was found to occur in 4-5 layers. It should be noted that the deposition of the enzyme in ideal monolayers should be considered optimal, since no screening of the active centers or blockage of sites by upper layers will occur.…”

Section: Morphology and Laccase Content Of The Pa6-laccase Conjugatesmentioning

confidence: 99%

“…Laccase-adsorbed PA6 MP were used for the development of optical biosensor for catechol determination with a detection limit of 11 μM and responding linearly to up to 118 μM of catechol [40]. With some modification of the published procedure [39], AAROP of γ-butyrolactam was employed by the same research group to produce neat and magnetic responsive PA4 MP that proved to be effective supports of model proteins [41], in molecular imprinting toward materials for protein recognition [42], or in laccase-containing biocatalysts for dye discoloration [43]. It is worth noting that chemically the polyamide micro particular supports and biomolecules such as proteins, enzymes, RNA oligonucleotides etc.…”

Section: Introductionmentioning

confidence: 99%

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Magnetically Responsive PA6 Microparticles with Immobilized Laccase Show High Catalytic Efficiency in the Enzymatic Treatment of Catechol

et al. 2021

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Herewith we report the first attempt towards non-covalent immobilization of Trametes versicolor laccase on neat and magnetically responsive highly porous polyamide 6 (PA6) microparticles and their application for catechol oxidation. Four polyamide supports, namely neat PA6 and such carrying Fe, phosphate-coated Fe and Fe3O4 cores were synthesized in suspension by activated anionic ring-opening polymerization (AAROP) of ε-caprolactam (ECL). Enzyme adsorption efficiency up to 92% was achieved in the immobilization process. All empty supports and PA6 laccase complexes were characterized by spectral and synchrotron WAXS/SAXS analyses. The activity of the immobilized laccase was evaluated using 2,2’-Azino-bis-(3- ethylbenzothiazoline-6-sulfonic acid (ABTS) and compared to the native enzyme. The PA6 laccase conjugates displayed up to 105% relative activity at room temperature, pH 4, 40 °C and 20 mM ionic strength (citrate buffer). The kinetic parameters of the ABTS oxidation were also determined. The reusability of the immobilized laccase-conjugates was proven for five consecutive oxidation cycles of catechol.

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Section: Discussioncontrasting

confidence: 82%

Section: Ftir Spectroscopysupporting

confidence: 78%

Section: Enzymatic Activity Of Pa6@l Conjugatessupporting

confidence: 65%

Section: Morphology and Laccase Content Of The Pa6-laccase Conjugatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Magnetically Responsive PA6 Microparticles with Immobilized Laccase Show High Catalytic Efficiency in the Enzymatic Treatment of Catechol

et al. 2021

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Synthesis and properties of neat, hybrid, and copolymeric polyamide 12 microparticles and composites on their basis

Dencheva

Braz

Denchev

2021

J of Applied Polymer Sci

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Neat and hybrid polyamide 12 (PA12) based powders are important raw materials for additive manufacturing. This study describes a facile method for the synthesis of pure, hybrid and copolymeric microparticulate materials based on one‐pot activated anionic ring‐opening polymerization (AAROP) of laurolactam in solution. The results reveal the possibility to obtain in good yields, for reaction times of 2 h and temperatures of up to 110°C neat and hybrid PA12 microparticles (MP) carrying metal or carbon fillers. Copolymeric PA12/PA6 MP can also be successfully prepared. All MP materials are analyzed by spectral, microscopy, thermal, and synchrotron X‐ray scattering methods in order to clarify their morphology, chemical and crystalline structure, melting and degradation behavior. The melting temperature of the PA12 MP is lower than of the commercial PA12, the presence of metal particles or copolymerization with PA6 additionally decrease it well below 170°C. The composites prepared by compression molding of PA12 MP display elastic modulus of up to 1.49 GPa, the stresses at yield and break reaching 50 and 69 MPa, respectively. It is concluded that the neat, hybrid, and copolymeric pulverulent materials obtained via microencapsulation by AAROP in solution may be useful in additive manufacturing processing.

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Characterization of biodegradable food packaging films prepared with polyamide 4: Influence of molecular weight and environmental humidity

Zhang

Nie

Zhang

et al. 2022

Food Bioengineering

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The development of biodegradable packaging materials focuses on their barrier properties and mechanical properties, which are critical parameters for food protection. Synthetic biodegradable material polyamide 4 (PA4) may offer outstanding capacities for packaging due to its rigid molecular structure. The current study originally reveals the various physical properties of PA4 films and explores the influence of its internal structure, molecular weight, and external environment, environmental humidity, on its performance to provide further guidance for PA4 application. PA4 (Mη = 18,000 g/mol) films showed tensile strength (TS) of 47.5 ± 1.3 MPa, elongation at break (EB) of 228% ± 30%, oxygen permeability of 7.83 ± 3.32 × 10−17 cm3 m−1 s−1 Pa−1, and water vapor permeability of 4.34 ± 0.06 × 10−11g m−1 s−1 Pa−1. The TS and EB of PA4 improved slightly with increasing molecular weight, while the barrier characteristic remained unchanged. The moisture adsorption capabilities of PA4 films were similar to natural hydrophilic polymers. The mechanical characteristic of PA4 changed from brittle to ductile with the increase in relative humidity. Overall, PA4 can be considered to be a promising food packaging material since it presented better mechanical and oxygen barrier properties than those of commercial food packaging polymers.

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Polymer-Assisted Biocatalysis: Polyamide 4 Microparticles as Promising Carriers of Enzymatic Function

Cited by 14 publications

References 48 publications

Magnetically Responsive PA6 Microparticles with Immobilized Laccase Show High Catalytic Efficiency in the Enzymatic Treatment of Catechol

Magnetically Responsive PA6 Microparticles with Immobilized Laccase Show High Catalytic Efficiency in the Enzymatic Treatment of Catechol

Synthesis and properties of neat, hybrid, and copolymeric polyamide 12 microparticles and composites on their basis

Characterization of biodegradable food packaging films prepared with polyamide 4: Influence of molecular weight and environmental humidity

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