Fabrication and evaluation of combined 3D printed/pamidronate-layered double hydroxides enriched electrospun scaffolds for bone tissue engineering applications

Belgheisi, Ghazal; Nazarpak, Masoumeh Haghbin; Solati‐Hashjin, Mehran

doi:10.1016/j.clay.2022.106538

Cited by 13 publications

(6 citation statements)

References 47 publications

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“…It is now understood that fibroblasts in the dermis deposit within pores composed of dermal fibers. Smaller pore diameters in dermal scaffolds pose challenges for fibroblast adhesion and migration in dermal pores ( Ghazal et al, 2022 ; Zohaib et al, 2022 ; Olevsky et al, 2023 ). Our reconstruction of the three-dimensional structure of normal human dermis through continuous tissue sectioning techniques yielded an average pore diameter of 219.29 ± 34.27 μm.…”

Section: Discussionmentioning

confidence: 99%

Three-dimensional model of normal human dermal tissue using serial tissue sections

Liu,

Zhang,

Huang

2024

Front. Bioeng. Biotechnol.

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Background: This study aims to construct a three-dimensional model of skin dermis utilizing continuous tissue sections, with the primary objective of obtaining anatomical structure data for normal human dermal tissues.Methods: Normal skin tissue specimens were acquired, paraffin-embedded, and subjected to HE staining. Panoramic images of skin sections were captured using a microscope. Tissue section images were aligned using the SIFT and StackReg image alignment methods, with analysis conducted using the OpenCV module. Mimics17 software facilitated the reconstruction of the skin dermal 3D model, enabling the calculation of dermal porosity and the void diameter.Results: Panoramic skin slices exhibited high-resolution differentiation of dermal fibers and cellular structures. Both SIFT and StackReg image alignment methods yielded similar results, although the SIFT method demonstrated greater robustness. Successful reconstruction of the three-dimensional dermal structure was achieved. Quantitative analysis revealed a dermal porosity of 18.96 ± 4.41% and an average pore diameter of 219.29 ± 34.27 μm. Interestingly, the porosity of the dermis exhibited a gradual increase from the papillary layer to the fourth layer, followed by a transient decrease and then a gradual increase. The distribution of the mean pore diameter mirrored the pattern observed in porosity distribution.Conclusion: Utilizing the continuous skin tissue slice reconstruction technique, this study successfully reconstructed a high-precision three-dimensional tissue structure of the skin. The quantitative analysis of dermal tissue porosity and average pore diameter provides a standardized dataset for the development of biomimetic tissue-engineered skin.

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Section: Discussionmentioning

confidence: 99%

Three-dimensional model of normal human dermal tissue using serial tissue sections

Liu,

Zhang,

Huang

2024

Front. Bioeng. Biotechnol.

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“…An example of these scaffolds, developed by Belgheisi et al [ 280 ], is shown in Figure 20 A. A 3D structure of PCL micro-strands grid was combined with nanofibers of PCL containing dispersed particles of pamidronate (Pam) drug intercalated into LDH (LDH-Pam) obtained by electrospinning (PCL/LDH-Pam).…”

Section: Organic Polymers and Layered Double Hydroxides Nanocomposite...mentioning

confidence: 99%

“… ( A ) Structural characterization of the PCL-based scaffolds: ( a ) macroscopic images; ( b ) top view (scale bar: 500 μm), ( c ) cross-sectional view (scale bar: 500 μm), and ( d ) top view of a pore of a 3D-printed grid (scale bar: 200 μm), and electrospun nanofibers (scale bar: 20 μm); ( B ) In vitro drug release: ( a ) cumulative release of Pam and ( b ) images of PCL/LDH-Pam fibers in the scaffold at the water penetration and degradation phases (scale bar: 200 μm), after 28 days of immersing in PBS solution (reprinted from Belgheisi et al [ 280 ] with permission of Elsevier). …”

Section: Figurementioning

confidence: 99%

Biomaterials Based on Organic Polymers and Layered Double Hydroxides Nanocomposites: Drug Delivery and Tissue Engineering

et al. 2023

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The development of biomaterials has a substantial role in pharmaceutical and medical strategies for the enhancement of life quality. This review work focused on versatile biomaterials based on nanocomposites comprising organic polymers and a class of layered inorganic nanoparticles, aiming for drug delivery (oral, transdermal, and ocular delivery) and tissue engineering (skin and bone therapies). Layered double hydroxides (LDHs) are 2D nanomaterials that can intercalate anionic bioactive species between the layers. The layers can hold metal cations that confer intrinsic biological activity to LDHs as well as biocompatibility. The intercalation of bioactive species between the layers allows the formation of drug delivery systems with elevated loading capacity and modified release profiles promoted by ion exchange and/or solubilization. The capacity of tissue integration, antigenicity, and stimulation of collagen formation, among other beneficial characteristics of LDH, have been observed by in vivo assays. The association between the properties of biocompatible polymers and LDH-drug nanohybrids produces multifunctional nanocomposites compatible with living matter. Such nanocomposites are stimuli-responsive, show appropriate mechanical properties, and can be prepared by creative methods that allow a fine-tuning of drug release. They are processed in the end form of films, beads, gels, monoliths etc., to reach orientated therapeutic applications. Several studies attest to the higher performance of polymer/LDH-drug nanocomposite compared to the LDH-drug hybrid or the free drug.

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“…Owing to the synergistic effect of bioprinter parameters and bioink properties, a precise experimental method should be used to determine the most compatible setting for the bioprinter. Similar to the Taguchi method, an experimental process is necessary to fabricate an accurate biological construct that can mimic genuine cell niches (Belgheisi et al , 2022; Asadi-Eydivand et al , 2018).…”

Section: Introductionmentioning

confidence: 99%

“…A larger nozzle diameter decreases the shear stress and cell damage while reducing resolution (Belgheisi et al , 2022). The shear stresses of the liquid flow are pressure-dependent.…”

Section: Introductionmentioning

confidence: 99%

Extrusion-based bioprinting: considerations toward gelatin-alginate bioink

Abedi,

Keshvari,

Solati-Hashjin

2024

RPJ

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Purpose This study aims to develop a simplified bioink preparation method that can be applied to most hydrogel bioinks used in extrusion-based techniques. Design/methodology/approach The parameters of the bioprinting process significantly affect the printability of the bioink and the viability of cells. In turn, the bioink formulation and its physicochemical properties may influence the appropriate range of printing parameters. In extrusion-based bioprinting, the rheology of the bioink affects the printing pressure, cell survival and structural integrity. Three concentrations of alginate-gelatin hydrogel were prepared and printed at three different flow rates and nozzle gauges to investigate the print parameters. Other characterizations were performed to evaluate the hydrogel structure, printability, gelation time, swelling and degradation rates of the bioink and cell viability. An experimental design was used to determine optimal parameters. The analyses included live/dead assays, rheological measurements, swelling and degradation. Findings The experimental design results showed that the hydrogel flow rate substantially influenced printing accuracy and pressure. The best hydrogel flow rate in this study was 10 ml/h with a nozzle gauge of 18% and 4% alginate. Three different concentrations of alginate-gelatin hydrogels were found to exhibit shear-thinning behavior during printing. After seven days, 46% of the structure in the 4% alginate-5% gelatin sample remained intact. After printing, the viability of skin fibroblast cells for the optimized sample was 91%. Originality/value This methodology offers a straightforward bioink preparation method applicable to the majority of hydrogels used in extrusion-based procedures. This can also be considered a prerequisite for cell printing.

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Fabrication and evaluation of combined 3D printed/pamidronate-layered double hydroxides enriched electrospun scaffolds for bone tissue engineering applications

Cited by 13 publications

References 47 publications

Three-dimensional model of normal human dermal tissue using serial tissue sections

Three-dimensional model of normal human dermal tissue using serial tissue sections

Biomaterials Based on Organic Polymers and Layered Double Hydroxides Nanocomposites: Drug Delivery and Tissue Engineering

Extrusion-based bioprinting: considerations toward gelatin-alginate bioink

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