Fabrication and characterization of poly(l-lactic acid) 3D nanofibrous scaffolds with controlled architecture by liquid–liquid phase separation from a ternary polymer–solvent system

He, Liumin; Zhang, Yanqing; Zeng, Xiangli; Quan, Daping; Liao, Susan; Zeng, Yuan-Shan; Lu, Jiang; Ramakrishna, Seeram

doi:10.1016/j.polymer.2009.06.025

Cited by 107 publications

(69 citation statements)

References 42 publications

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“…Among these steps, the gelation process, especially the T gel was critical in influencing the architecture of the scaffold (Ma and Zhang, 1999;He et al, 2009). Fig.…”

Section: Effects Of Gelation Temperature (T Gel ) On the Morphologiesmentioning

confidence: 99%

Microstructure and properties of nano-fibrous PCL-b-PLLA scaffolds for cartilage tissue engineering

Liu²,

Guan³

et al. 2009

eCM

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Nano-fibrous scaffolds which could potentially mimic the architecture of extracellular matrix (ECM) have been considered a good candidate matrix for cell delivery in tissue engineering applications. In the present study, a semicrystalline diblock copolymer, poly(ε-caprolactone)-block-poly(L-lactide) (PCL-b-PLLA), was synthesized and utilized to fabricate nano-fibrous scaffolds via a thermally induced phase separation process. Uniform nano-fibrous networks were created by quenching a PCL-b-PLLA/THF homogenous solution to -20ºC or below, followed by further gelation for 2 hours due to the presence of PLLA and PCL microcrystals. However, knot-like structures as well as continuously smooth pellicles appeared among the nanofibrous network with increasing gelation temperature. DSC analysis indicated that the crystallization of PCL segments was interrupted by rigid PLLA segments, resulting in an amorphous phase at high gelation temperatures. Combining TIPS (thermally induced phase separation) with saltleaching methods, nano-fibrous architecture and interconnected pore structures (144±36 μm in diameter) with a high porosity were created for in vitro culture of chondrocytes. Specific surface area and protein adsorption on the surface of the nano-fibrous scaffold were three times higher than on the surface of the solid-walled scaffold. Chondrocytes cultured on the nano-fibrous scaffold exhibited a spherical condrocyte-like phenotype and secreted more cartilage-like extracellular matrix (ECM) than those cultured on the solid-walled scaffold. Moreover, the protein and DNA contents of cells cultured on the nanofibrous scaffold were 1.2-1.4 times higher than those on the solid-walled scaffold. Higher expression levels of collagen II and aggrecan mRNA were induced on the nanofibrous scaffold compared to on the solid-walled scaffold. These findings demonstrated that scaffolds with a nanofibrous architecture could serve as superior scaffolds for cartilage tissue engineering.

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“…Among these steps, the gelation process, especially the T gel was critical in influencing the architecture of the scaffold (Ma and Zhang, 1999;He et al, 2009). Fig.…”

Section: Effects Of Gelation Temperature (T Gel ) On the Morphologiesmentioning

confidence: 99%

Microstructure and properties of nano-fibrous PCL-b-PLLA scaffolds for cartilage tissue engineering

Liu²,

Guan³

et al. 2009

eCM

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“…Similarly, the cytoprotective effect of HPC was demonstrated in a number of in vivo studies using clinically relevant ischaemic models (Table 2). In these studies, ex vivo HPC rendered stem and progenitor cells more resilient to cell death when transplanted into the infarcted myocardium (He et al, 2009;Hu et al, 2008;Tang et al, 2009;Uemura et al, 2006), ischaemic limb (Akita et al, 2003;Kubo et al, 2008;Li et al, 2002;Rosova et al, 2008) or ischaemic brain (Theus et al, 2008), and this finding was significantly correlated with improved functional recovery of the ischaemic tissues. For instance, transplantation of hypoxic preconditioned mesenchymal stem cells (MSCs) into the ischaemic myocardium showed enhanced therapeutic benefits in terms of infarct size reduction, increased angiogenesis, improved ventricular function and less adverse cardiac remodelling .…”

Section: Survivalmentioning

confidence: 99%

Advances in Regenerative Medicine

Clarke¹

2008

Regen. Med.

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“…As a function of the separations conditions, it is possible to obtain a wide range of different morphologies with various characteristics, such as: open or close pores, fibrous structure, membrane/like architecture, etc. [14][15][16][17].…”

Section: Thermally Induced Phase Separation (Tips) Techniquementioning

confidence: 99%

A Versatile Technique to Produce Porous Polymeric Scaffolds: The Thermally Induced Phase Separation (TIPS) Method

Conoscenti¹,

Carrubba²,

Brucato³

2017

Arch Chem Res

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Among the various scaffold fabrication techniques, thermally induced phase separation (TIPS) is one of the most versatile methods to produce porous polymeric scaffold and it has been largely used for its capability to produce highly porous and interconnected scaffolds. The scaffold architecture can be closely controlled by varying the process parameters, including polymer type and concentration, solvent/non-solvent ratio and thermal history. TIPS technique has been widely employed, also, to produce scaffolds with a hierarchical pore structure and composite polymeric matrix/inorganic filler foams.

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Fabrication and characterization of poly(l-lactic acid) 3D nanofibrous scaffolds with controlled architecture by liquid–liquid phase separation from a ternary polymer–solvent system

Cited by 107 publications

References 42 publications

Microstructure and properties of nano-fibrous PCL-b-PLLA scaffolds for cartilage tissue engineering

Microstructure and properties of nano-fibrous PCL-b-PLLA scaffolds for cartilage tissue engineering

Advances in Regenerative Medicine

A Versatile Technique to Produce Porous Polymeric Scaffolds: The Thermally Induced Phase Separation (TIPS) Method

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