Fabrication and characterization of PCL/gelatin composite nanofibrous scaffold for tissue engineering applications by electrospinning method

Gautam, Sneh; Dinda, Amit Kumar; Mishra, Narayan Chandra

doi:10.1016/j.msec.2012.12.015

Cited by 463 publications

(309 citation statements)

References 23 publications

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“…Figure 3 shows FTIR spectra of nano-and micro-fibrous scaffolds as well as of pure n-HAp powder. Several characteristic bands of PCL were observed for both types of nanoand micro-fibrous samples at 1727 cm -1 (C=O stretching), 1293 cm -1 (C-O and C-C stretching), 1240 cm -1 (C-O-C asymmetric stretching), and 1175 cm -1 (C-O-C symmetric stretching) [36]. In the case of n-HAp modified scaffolds, some bands characteristic of n-HAp were identified.…”

Section: Resultsmentioning

confidence: 99%

Fabrication of bioactive polycaprolactone/hydroxyapatite scaffolds with final bilayer nano-/micro-fibrous structures for tissue engineering application

Rajzer

2014

J Mater Sci

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In this study, two techniques, namely electrospinning and needle-punching processes, were used to fabricate bioactive polycaprolactone/hydroxyapatite scaffolds with a final bilayer nano-/micro-fibrous porous structure. A hybrid scaffold was fabricated to combine the beneficial properties of nanofibers and microfibers and to create a three-dimensional porous structure (which is usually very difficult to produce using electrospinning technology only). The first part of this work focused on determining the conditions necessary to fabricate nano-and micro-fibrous components of scaffold layers. A characterization of scaffold components, with respect to their morphology, fiber diameter, pore size, wettability, chemical composition and mechanical properties, was performed. Then, the same process parameters were applied to produce a hybrid bilayer scaffold by electrospinning the nanofibers directly onto the micro-fibrous nonwovens obtained in a traditional mechanical needle-punching process. In the second part, the bioactive character of a hybrid nano-/ micro-fibrous scaffold in simulated body fluid (SBF) was assessed. Spherical calcium phosphate was precipitated onto the nano-/micro-fibrous scaffold surface proving its bioactivity.

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Section: Resultsmentioning

confidence: 99%

Fabrication of bioactive polycaprolactone/hydroxyapatite scaffolds with final bilayer nano-/micro-fibrous structures for tissue engineering application

Rajzer

2014

J Mater Sci

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“…Several studies have investigated PCL-Gel electrospun scaffolds. [17][18][19][20] This approach of using a synthetic/natural polymer blend overcomes the drawback of individual synthetic and natural polymers and improves mechanical properties and biocompatibility.…”

mentioning

confidence: 99%

In VitroandIn VivoStudies of BMP-2-Loaded PCL–Gelatin–BCP Electrospun Scaffolds

Kim

Linh

Min

et al. 2014

Tissue Engineering Part A

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To confirm the effect of recombinant human bone morphogenetic protein-2 (BMP-2) for bone regeneration, BMP-2-loaded polycaprolactone (PCL)-gelatin (Gel)-biphasic calcium phosphate (BCP) fibrous scaffolds were fabricated using the electrospinning method. The electrospinning process to incorporate BCP nanoparticles into the PCL-Gel scaffolds yielded an extracellular matrix-like microstructure that was a hybrid system composed of nano-and micro-sized fibers. BMP-2 was homogeneously loaded on the PCL-Gel-BCP scaffolds for enhanced induction of bone growth. BMP-2 was initially released at high levels, and then showed sustained release behavior for 31 days. Compared with the PCL-Gel-BCP scaffold, the BMP-2-loaded PCL-Gel-BCP scaffold showed improved cell proliferation and cell adhesion behavior. Both scaffold types were implanted in rat skull defects for 4 and 8 weeks to evaluate the biological response under physiological conditions. Remarkable bone regeneration was observed in the BMP-2/PCL-Gel-BCP group. These results suggest that BMP-2-loaded PCL-Gel-BCP scaffolds should be considered for potential bone tissue engineering applications.

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“…The XRD pattern of blank nanoparticles (PCL-NP) showed two peaks related to semi crystalline polymer structure (41). Nisin-PCL physical mixture XRD pattern showed signals related both to the crystalline structure of the peptide as well the semi crystalline structure of PCL (Fig.…”

Section: Resultsmentioning

confidence: 98%

Development and Characterization of Nisin Nanoparticles as Potential Alternative for the Recurrent Vaginal Candidiasis Treatment

et al. 2016

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Abstract. The aim of this work was the development and characterization of nisin-loaded nanoparticles and the evaluation of its potential antifungal activity. Candidiasis is a fungal infection caused by Candida sp. considered as one of the major public health problem currently. The discovery of antifungal agents that present a reduced or null resistance of Candida sp. and the development of more efficient drug release mechanisms are necessary for the improvement of candidiasis treatment. Nisin, a bacteriocin commercially available for more than 50 years, exhibits antibacterial action in food products with potential antifungal activity. Among several alternatives used to modulate antifungal activity of bacteriocins, polymeric nanoparticles have received great attention due to an effective drug release control and reduction of therapeutic dose, besides the minimization of adverse effects by the preferential accumulation in specific tissues. The nisin nanoparticles were prepared by double emulsification and solvent evaporation methods. Nanoparticles were characterized by dynamic light scattering, zeta potential, Fourier transform infrared, X-ray diffraction, differential scanning calorimetry, and scanning electron microscopy. Antifungal activity was accessed by pour plate method and cell counting using Candida albicans strains. The in vitro release profile and in vitro permeation studies were performed using dialysis bag method and pig vaginal mucosa in Franz diffusion cell, respectively. The results revealed nisin nanoparticles (300 nm) with spherical shape and high loading efficiency (93.88 ± 3.26%). In vitro test results suggest a promising application of these nanosystems as a prophylactic agent in recurrent vulvovaginal candidiasis and other gynecological diseases.

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Fabrication and characterization of PCL/gelatin composite nanofibrous scaffold for tissue engineering applications by electrospinning method

Cited by 463 publications

References 23 publications

Fabrication of bioactive polycaprolactone/hydroxyapatite scaffolds with final bilayer nano-/micro-fibrous structures for tissue engineering application

Fabrication of bioactive polycaprolactone/hydroxyapatite scaffolds with final bilayer nano-/micro-fibrous structures for tissue engineering application

In VitroandIn VivoStudies of BMP-2-Loaded PCL–Gelatin–BCP Electrospun Scaffolds

Development and Characterization of Nisin Nanoparticles as Potential Alternative for the Recurrent Vaginal Candidiasis Treatment

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