In VitroandIn VivoStudies of BMP-2-Loaded PCL–Gelatin–BCP Electrospun Scaffolds

Abstract

To confirm the effect of recombinant human bone morphogenetic protein-2 (BMP-2) for bone regeneration, BMP-2-loaded polycaprolactone (PCL)-gelatin (Gel)-biphasic calcium phosphate (BCP) fibrous scaffolds were fabricated using the electrospinning method. The electrospinning process to incorporate BCP nanoparticles into the PCL-Gel scaffolds yielded an extracellular matrix-like microstructure that was a hybrid system composed of nano-and micro-sized fibers. BMP-2 was homogeneously loaded on the PCL-Gel-BCP scaffolds for enhanced induction of bone growth. BMP-2 was initially released at high levels, and then showed sustained release behavior for 31 days. Compared with the PCL-Gel-BCP scaffold, the BMP-2-loaded PCL-Gel-BCP scaffold showed improved cell proliferation and cell adhesion behavior. Both scaffold types were implanted in rat skull defects for 4 and 8 weeks to evaluate the biological response under physiological conditions. Remarkable bone regeneration was observed in the BMP-2/PCL-Gel-BCP group. These results suggest that BMP-2-loaded PCL-Gel-BCP scaffolds should be considered for potential bone tissue engineering applications.

Effect of Local Sustainable Release of BMP2-VEGF from Nano-Cellulose Loaded in Sponge Biphasic Calcium Phosphate on Bone Regeneration

“…[17][18][19][20][21][22] Bone regeneration process is accomplished by the active participation of different growth factors.…”

Nanotechnology in bone tissue engineering

“…There have been new techniques to overcome the hydrophobic nature of polymeric materials using surfactants, 113 new scaffolds tailored to specific application such as aligned nanoyarn reinforcement for tendon repair, 115 new structural formations like that of cotton wool, 116 new composite combinations like chitosan (CS) and silk fibroin (SF), 117 and new confirmatory studies of the bone stimulating effects of known pro-osteogenic proteins like BMP-2 within the context of electrospun scaffolds. 118 Electrospun scaffolds are typically limited for bone tissue replacement due to low mechanical strength. Recent progress on this front has been made with electrospun silk fibroin scaffolds through uniform dispersion of hydroxyapatite nanoparticles to increase tensile strength.…”

Nanotechnology for biomedical applications: Recent advances in neurosciences and bone tissue engineering

“…The process of fabricating scaffolds with electrospinning has seen many innovations. There have been new techniques to overcome the hydrophobic nature of polymeric materials using surfactants, new structural formations like that of cotton wool, new composite combinations like chitosan and silk fibroin, and new confirmatory studies of the bone stimulating effects of known pro‐osteogenic proteins like BMP‐2 .…”