Fabrication and characterization of highly porous barium titanate based scaffold coated by Gel/HA nanocomposite with high piezoelectric coefficient for bone tissue engineering applications

Ehterami, Arian; Kazemi, Mohammad Hossein; Nazari, Bahareh; Saraeian, Payam; Azami, Mahmoud

doi:10.1016/j.jmbbm.2017.12.034

Cited by 82 publications

(59 citation statements)

References 31 publications

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“…Postfixation was performed in 1% Osmium tetroxide, and dehydration in a graded series of alcohols (70%, 80%, 96%, and two changes of 100% ethanol). Samples were then freeze‐dried and kept dry using silica gel . Dried samples were coated with a thin layer of Gold (Au) and then the morphology of the cells was observed using a scanning electron microscope (Philips XL30, Netherland) that operated at the acceleration voltage of 25 kV.…”

Section: Methodsmentioning

confidence: 99%

Bone Regeneration in rat using a gelatin/bioactive glass nanocomposite scaffold along with endothelial cells (HUVECs)

Kazemi

Azami

Johari

et al. 2018

Int J Applied Ceramic Tech

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In our previous study, a three‐dimensional gelatin/bioactive glass nanocomposite scaffold with a total porosity of about 85% and pore sizes ranging from 200 to 500 μm was prepared through layer solvent casting combined with lamination technique. The aim of this study was to evaluate in vitro biocompatibility and in vivo bone regeneration potential of these scaffolds with and without endothelial cells when implanted into a critical‐sized rat calvarial defect. MTT assay, SEM observation, and DAPI staining were used to evaluate cell viability and adhesion in macroporous scaffolds and results demonstrated that the scaffolds were biocompatible enough to support cell attachment and proliferation. To investigate the in vivo osteogenesis of the scaffold, blank scaffolds and endothelial/scaffold constructs were implanted in critical‐sized defects, whereas in control group defects were left untreated. Bone regeneration and vascularization were evaluated at 1, 4, and 12 weeks postsurgery by histological, immunohistochemical, and histomorphometric analysis. It was shown that both groups facilitated bone growth into the defect area but improved bone regeneration was seen with the incorporation of endothelial cells. The data showed that the porous Gel/BaG nanocomposite scaffolds could well support new bone formation, indicating that the proposed strategy is a promising alternative for tissue‐engineered bone defects.

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Section: Methodsmentioning

confidence: 99%

Bone Regeneration in rat using a gelatin/bioactive glass nanocomposite scaffold along with endothelial cells (HUVECs)

Kazemi

Azami

Johari

et al. 2018

Int J Applied Ceramic Tech

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“…Barium titanate nanoparticles have been shown to enhance osteogenic differentiation of mesenchymal stem cells [78,79], and these results provide for new approaches in bone tissue engineering. Furthermore, Ehterami et al reported that highly porous BT scaffolds coated with Gel/HA nanocomposites presented with good biocompatibility, and MG63 osteoblast-like cells exhibited better adhesion, proliferation, and migration into the pores of these scaffolds [80]. In recent years, HA/BT (hydroxyapatite/barium titanate) composites have gathered significant interest within the scientific community for use in the design of scaffolds for bone substitutes owing to their bioactivity and osteogenic capabilities [81][82][83][84] (Figure 10).…”

Section: Piezoceramicsmentioning

confidence: 99%

The application of nanogenerators and piezoelectricity in osteogenesis

Kao

Chiu

Tsai

et al. 2019

Science and Technology of Advanced Materials

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Bone is a complex organ possessing both physicomechanical and bioelectrochemical properties. In the view of Wolff's Law, bone can respond to mechanical loading and is subsequently reinforced in the areas of stress. Piezoelectricity is one of several mechanical responses of the bone matrix that allows osteocytes, osteoblasts, osteoclasts, and osteoprogenitors to react to changes in their environment. The present review details how osteocytes convert external mechanical stimuli into internal bioelectrical signals and the induction of intercellular cytokines from the standpoint of piezoelectricity. In addition, this review introduces piezoelectric and triboelectric materials used as self-powered electrical generators to promote osteogenic proliferation and differentiation due to their electromechanical properties, which could promote the development of promising applications in tissue engineering and bone regeneration. ARTICLE HISTORY

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“…By using biomaterial scaffolds, tissue engineering provides an alternative approach to improve survival and integration of transplanted cells along with their proper differentiation. In fact, scaffolds meet these goals by providing a proper matrix that mimics the extracellular matrix (ECM) . The biomaterial should have mechanical characteristics of the host tissue and be biocompatible and biodegradable in order to be used for the cell therapy of the CNS .…”

Section: Introductionmentioning

confidence: 99%

“…In fact, scaffolds meet these goals by providing a proper matrix that mimics the extracellular matrix (ECM). [13][14][15][16][17][18] The biomaterial should have mechanical characteristics of the host tissue and be biocompatible and biodegradable in order to be used for the cell therapy of the CNS. 14,19 One of the widely used biomaterials is hydrogel.…”

Section: Introductionmentioning

confidence: 99%

Fibrin hydrogel as a scaffold for differentiation of induced pluripotent stem cells into oligodendrocytes

et al. 2019

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The importance of tissue engineering has been established as a promising approach in treating neurodegenerative diseases. The purpose of the current study is to determine the effect of fibrin hydrogel on the differentiation of iPSC into oligodendrocyte. For this purpose, iPSCs transduced by miR‐338 expressing lentiviruses. They were treated with basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), and platelet‐derived growth factor (PDGF)‐AA. The process was traced by a 6‐day treatment in a mitogen‐free medium. At the end of the process, multipolar preoligodendrocytes appeared. In comparison to tissue culture plate (TCP), MTT assay demonstrated a significant increase in the viability of cells cultured in fibrin hydrogel. SEM analysis showed cells with elongated morphology and intertwined intercellular interactions. An immunofluorescent assay confirmed the expression of oligodendrocyte markers Olig2 and O4. In comparison to TCP, real‐time PCR data indicated a significant increase in the expression of some markers such as Olig2, MBP, Sox10, and PDGFRα on cells encapsulated in fibrin hydrogel. Overall, the results suggest that fibrin hydrogel improves viability of cells and promotes the differentiation of iPSCs into preoligodendrocytes. Hence, it can be used as an appropriate option in the tissue engineering in order to treat neurodegenerative diseases. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:192–200, 2020.

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Fabrication and characterization of highly porous barium titanate based scaffold coated by Gel/HA nanocomposite with high piezoelectric coefficient for bone tissue engineering applications

Cited by 82 publications

References 31 publications

Bone Regeneration in rat using a gelatin/bioactive glass nanocomposite scaffold along with endothelial cells (HUVECs)

Bone Regeneration in rat using a gelatin/bioactive glass nanocomposite scaffold along with endothelial cells (HUVECs)

The application of nanogenerators and piezoelectricity in osteogenesis

Fibrin hydrogel as a scaffold for differentiation of induced pluripotent stem cells into oligodendrocytes

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