Fabrication and characterization of electrospun polyurethane blended with dietary grapes for skin tissue engineering

Abstract

The tissue-engineered skin has emerged as a plausible alternative approach to the traditional wound dressing owing to its inherent advantages. Further, in tissue engineering applications, fibrous scaffold obtained through textile technologies is widely attractive. The present study focused on the fabrication of electrospun textile polyurethane wound dressing scaffold incorporated with grape extract. The fabricated composites showed smooth as well as reduced fiber (730 ± 127 nm) and pore (873 ± 51 nm) diameter than the control polyurethane (fiber diameter –890 ± 117 and pore diameter –1064 ± 74 nm) as revealed in the scanning electron microscopy. The formation of hydrogen bond in Fourier transform infrared spectroscopy revealed the interaction between the polyurethane and grape. The addition of grape enhanced the wettability behavior (86° ± 2) and the surface roughness (469 nm) of the polyurethane membrane. Thermal gravimetric analysis and mechanical testing revealed the enhancement of thermal and tensile strength with the incorporation of the grape into the polyurethane matrix. The in vitro blood compatibility and cytocompatibility studies revealed enhanced antithrombogenicity behavior and the non-toxic nature to human dermal fibroblast cells for the fabricated composites than the pristine polyurethane. Hence, the addition of grape into the polyurethane matrix had enhanced the physicochemical characteristics and biocompatibility parameters which could promote this candidate as a valid alternative for skin tissue engineering regeneration.

Fabrication and Characterization of Fibrous Polycaprolactone Blended with Natural Green Tea Extracts Using Dual Solvent Systems

“…Water contact angle measurement of the PCL/green tea membrane is reduced indicating the wettability of the composite membrane is enhanced because of the incorporation of green tea extract into the PCL solution. It has been reported that smaller the fiber diameter wettability of the membrane will be enhanced [5,6]. Based on the SEM analysis, PCL/green tea composite had reduced fiber diameter, which has a putative role in the contact angle reduction.…”

“…Scanning electron microscopy (SEM) was used to analyze the morphology of fi ber produced [5,6]. SEM unit (Hitachi Tabletop TM3000, Tokyo, Japan) was used to obtain the SEM image of the fabricated fi brous membrane.…”

“…To analyze the wettability of the fabricated fi brous membrane [5,6], the water contact angle was measured. The VCA Optima contact angle measurement unit (AST Products, Inc., Billerica, MA, USA) was used in this study.…”

“…Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) analysis was performed to study the chemical composition of the fabricated fibrous membranes [5]. The ATR-FTIR unit (Nicolet IS5, Thermo Fischer Scientific, Waltham, MA, USA) was used to measure the spectra of each sample.…”

“…with grapes [5] and garlic [6] were manufactured, and they were found to possess conductive properties necessary for wound healing application. Similarly, a recent work fabricated polycaprolactone (PCL) blended with aloe vera for guided tissue regeneration.…”