Abstract:Core–shell microparticles containing an aqueous core have demonstrated their value for microencapsulation and drug delivery systems. The most important step in generating these uniquely structured microparticles is the formation of droplets and double emulsion. The droplet generator must meet the performance and reliability requirements, including accurate size control with tunability and monodispersity. Herein, we present a facile technique to generate surfactant-free core–shell droplets with an aqueous core … Show more
“…As a platform of digital micro elastofluidics, liquid beads represent a potentially practical solution for storage and precise metering of reagents on a wearable device. Both oil-based 129 and aqueous liquids 130 can be encapsulated in a solid polymer shell to form liquid beads with outer diameters on the order of 100 μm. In contrast to conventional liquid handling, dosing and metering of reagents can be simply implemented with the number of liquid beads.…”
Section: Microfluidic Solutions For Controlling and Manipulating Biof...mentioning
“…As a platform of digital micro elastofluidics, liquid beads represent a potentially practical solution for storage and precise metering of reagents on a wearable device. Both oil-based 129 and aqueous liquids 130 can be encapsulated in a solid polymer shell to form liquid beads with outer diameters on the order of 100 μm. In contrast to conventional liquid handling, dosing and metering of reagents can be simply implemented with the number of liquid beads.…”
Section: Microfluidic Solutions For Controlling and Manipulating Biof...mentioning
“…Polymerization and subsequent solidification of the shell prevented the coalescence of sample droplets during the subsequent PCR process and ensured their long-term stability. 46,47 In the present study, we utilised several liquid systems to study the impact of material properties on the position of the core in the shell droplet. We first studied the surfactant concentration in the continuous phases introduced from inlets 3 and 4 as the spacer fluid, while deionized water was used as the core phase.…”
Section: Preparation Of Core-shell Particlesmentioning
Droplet-based microfluidics and digital polymerase chain reaction (PCR) hold significant promise for accurately detecting and quantifying pathogens. However, existing droplet-based digital PCR (ddPCR) applications have been relying exclusively on single...
“…Moreover, core-shell beads remain stable without merging during thermocycling. 18 The present work describes the use of core-shell beads as microchambers for dPCR and showcases a cost-effective, wide-field imaging technique for its evaluation. We report an image analysis method for liquid beads which eliminates the need for corrections related to lens distortion, skewness, or non-uniform illumination.…”
Section: Introductionmentioning
confidence: 99%
“…The robust polymer shell ensures easy handling and safeguards against physical rupturing. 18 These features enable long term storage of the samples. Following the PCR process, the samples can also be retrieved for further analysis.…”
Section: Introductionmentioning
confidence: 99%
“…A flow-focusing microfluidic device uses trimethylolpropane trimethacrylate (TMPTMA) as shell phase and water as core phase. The properties of TMPTMA such as its transparency, 21 thermal stability, 18 non-toxicity, 22 and biocompatibility, 23 make it highly suitable for PCR applications. Our image acquisition setup consists of a 21-megapixel dSLR camera equipped with a 25 mm macro-lens at 2.5× to 5× magnification for capturing an area of 20 mm 2 .…”
Digital droplet reactors have become a valuable tool for the analysis of single cells, organisms, or molecules by discretising reagents into picolitre or nanolitre volumes. However, deoxyribonucleic acid (DNA) based...
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