Microfluidic encapsulation of DNAs in liquid beads for digital PCR application

Galogahi, Fariba Malekpour; Christie, Melodie; Yadav, Ajeet Singh; An, Hongjie; Stratton, Helen; Nguyen, Nam‐Trung

doi:10.1039/d3an00868a

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2024

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Cited by 2 publications

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“…Following the collection, the formed core–shell droplets are exposed to a 24 W blue light (450–490 nm) source for 20 minutes to polymerize and form liquid beads, solid particles with a liquid core. 24 However, the throughput is lower than conventional ddPCR methods. 25 This is attributed to slower optimised flow rate and about 50% of the produced beads are core–shell.…”

Section: Methodsmentioning

confidence: 99%

Precise, wide field, and low-cost imaging and analysis of core–shell beads for digital polymerase chain reaction

et al. 2023

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Section: Methodsmentioning

confidence: 99%

Precise, wide field, and low-cost imaging and analysis of core–shell beads for digital polymerase chain reaction

et al. 2023

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Synthesis and active manipulation of magnetic liquid beads

Yadav,

Galogahi,

Vashi

et al. 2024

Biomed Microdevices

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We report the fabrication and characterisation of magnetic liquid beads with a solid magnetic shell and liquid core using microfluidic techniques. The liquid beads consist of a fluorinated oil core and a polymer shell with magnetite particles. The beads are generated in a flow-focusing polydimethylsiloxane (PDMS) device and cured by photo polymerisation. We investigated the response of the liquid beads to an external magnetic field by characterising their motion towards a permanent magnet. Magnetic sorting of liquid beads in a channel was achieved with 90% efficiency. The results show that the liquid beads can be controlled magnetically and have potential applications in digital microfluidics including nucleic acid amplification, drug delivery, cell culture, sensing, and tissue engineering. The present paper also discusses the magnetophoretic behaviour of the liquid bead by varying its mass and magnetite concentration in the shell. We also demonstrated the two-dimensional self-assembly of magnetic liquid beads for potential use in digital polymerase chain reaction and digital loop mediated isothermal amplification. Graphical abstract

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Microfluidic encapsulation of DNAs in liquid beads for digital PCR application

Abstract: Droplet-based microfluidics and digital polymerase chain reaction (PCR) hold significant promise for accurately detecting and quantifying pathogens. However, existing droplet-based digital PCR (ddPCR) applications have been relying exclusively on single...

Cited by 2 publications

References 54 publications

Precise, wide field, and low-cost imaging and analysis of core–shell beads for digital polymerase chain reaction

Precise, wide field, and low-cost imaging and analysis of core–shell beads for digital polymerase chain reaction

Synthesis and active manipulation of magnetic liquid beads

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