Fabricating tubular scaffolds with a radial pore size gradient by a spinning technique

Harley, Brendan A.C.; Hastings, Abel Z.; Yannas, Ioannis V.; Sannino, Alessandro

doi:10.1016/j.biomaterials.2005.07.012

Cited by 121 publications

(95 citation statements)

References 19 publications

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“…It should be noted that previous studies have found that pore size reduces with T f as low as À808C. 10,29 However, the cooling rates used in these studies are greater (>18C=min) than the rate used in this study. This permits T f to influence both the shape of the ice crystals as they nucleate and the subsequent growth through diffusion.…”

Section: Novel Freeze-drying Methods Applied To Collagen-gag Scaffoldsmentioning

confidence: 58%

Novel Freeze-Drying Methods to Produce a Range of Collagen–Glycosaminoglycan Scaffolds with Tailored Mean Pore Sizes

Haugh

Murphy

O’Brien

2010

Tissue Engineering Part C: Methods

217

192

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• to copy, distribute, display, and perform the work.• to make derivative works. Under the following conditions:• Attribution -You must give the original author credit.• Non-Commercial -You may not use this work for commercial purposes.• The pore structure of three-dimensional scaffolds used in tissue engineering has been shown to significantly influence cellular activity. As the optimal pore size is dependant on the specifics of the tissue engineering application, the ability to alter the pore size over a wide range is essential for a particular scaffold to be suitable for multiple applications. With this in mind, the aim of this study was to develop methodologies to produce a range of collagen-glycosaminoglycan (CG) scaffolds with tailored mean pore sizes. The pore size of CG scaffolds is established during the freeze-drying fabrication process. In this study, freezing temperature was varied (À108C to À708C) and an annealing step was introduced to the process to determine their effects on pore size. Annealing is an additional step in the freeze-drying cycle that involves raising the temperature of the frozen suspension to increase the rate of ice crystal growth. The results show that the pore size of the scaffolds decreased as the freezing temperature was reduced. Additionally, the introduction of an annealing step during freeze-drying was found to result in a significant increase (40%) in pore size. Taken together, these results demonstrate that the methodologies developed in this study can be used to produce a range of CG scaffolds with mean pore sizes from 85 to 325 mm. This is a substantial improvement on the range of pore sizes that were possible to produce previously (96-150 mm). The methods developed in this study provide a basis for the investigation of the effects of pore size on both in vitro and in vivo performance and for the determination of the optimal pore structure for specific tissue engineering applications.

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Section: Novel Freeze-drying Methods Applied To Collagen-gag Scaffoldsmentioning

confidence: 58%

Novel Freeze-Drying Methods to Produce a Range of Collagen–Glycosaminoglycan Scaffolds with Tailored Mean Pore Sizes

Haugh

Murphy

O’Brien

2010

Tissue Engineering Part C: Methods

217

192

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“…However, less acute ankle joint angles observed in PVACNTs-MSCs group, compared with PVA-PPy and PVA-CNTs groups might suggest improved ankle muscles function during the push off phase of the rat's gait cycle ( Figure 4 and Table 3). It is well known and demonstrated by the scientific bibliography that neuromuscular pathologies are related to important clinical signs or motor deficits that should be observed, qualified, and quantified, only possible with a precise kinematic analysis [65,66] . In the field of peripheral nerve research using the rat sciatic nerve model, an improved walking analysis might include several methods combination like joint kinematics, ground reaction forces and electromyographical data of muscle activity.…”

Section: Discussionmentioning

confidence: 99%

“…In the field of peripheral nerve research using the rat sciatic nerve model, an improved walking analysis might include several methods combination like joint kinematics, ground reaction forces and electromyographical data of muscle activity. These methods refinements might be important to differentiate the regenerative potential of different scaffolds used, that are not evident when using the traditional standardized methods previously referred [9,66] . The recent published paper by di Summa et al [67] where collagen tube-guides (Neurogen ® ) were used in vivo to promote peripheral nerve regeneration, combined with schwann cells (SCs), it was possible to demonstrate an improved distal stump sprouting [68] .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells

Ribeiro¹,

Pereira²,

Caseiro³

et al. 2015

WJSC

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“…Numerous research activities are underway to create biomimetic templates with a wide range of geometries for biological and medical applications. 1 An ideal bioresorbable scaffold provides a substrate for cell growth while maintaining cell activity, leading to a self-supporting structure. The subsequent degradation of the scaffold itself must not cause harm to the growing cells and surrounding tissues.…”

Section: Introductionmentioning

confidence: 99%

Layered Open Pore Poly(l-lactic acid) Nanomorphology

et al. 2006

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Fabricating tubular scaffolds with a radial pore size gradient by a spinning technique

Cited by 121 publications

References 19 publications

Novel Freeze-Drying Methods to Produce a Range of Collagen–Glycosaminoglycan Scaffolds with Tailored Mean Pore Sizes

Novel Freeze-Drying Methods to Produce a Range of Collagen–Glycosaminoglycan Scaffolds with Tailored Mean Pore Sizes

Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells

Layered Open Pore Poly(l-lactic acid) Nanomorphology

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