Fabricated aptamer-based electrochemical “signal-off” sensor of ochratoxin A

Kuang, Hua; Chen, Wei; Xu, Dinghua; Xu, Liguang; Zhu, Yuntian; Liu, Liqiang; Chu, Hsin‐Sen; Peng, Chifang; Zhu, Shuifang

doi:10.1016/j.bios.2010.06.058

Cited by 203 publications

(91 citation statements)

References 46 publications

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“…Wang & Rando, 1995) Tobramycin 2'-OMe-RNA Electrochemical (Impedence) 0.7 µM (González-Fernández et al, 2011) RNA 180 -- (Kwon et al, 2001) Kanamycin RNA 10-30 -- (Goertz, Colin Cox Ellington, 2004b) RNA Low nM -- (Goertz, Colin Cox Ellington, 2004b) RNA 1800 -- (Cowan et al, 2000) Electrochemical (Impedence) "sub µM" (de-los-Santos-Alvarez et al, 2007) Neomycin 2'-OMe-RNA Surface Plasmon Resonance (SPR) 10 nM (de-los-Santos-Álvarez et al, 2009) - (Cruz-Aguado & Penner, 2008a) Fluorescence Polarization 5 nM (Cruz-Aguado & Penner, 2008b) Electrochemical 30 pg/mL (Kuang et al, 2010) Electrochemiluminescent 0.007 ng/mL (Z. Wang et al, 2010) Mycotoxins Ochratoxin A DNA 200…”

Section: Aminoglycosidesmentioning

confidence: 99%

Advances in Aptamer-Based Biosensors for Food Safety

McKeague¹,

Giamberardino²,

DeRosa³

2011

Environmental Biosensors

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Section: Aminoglycosidesmentioning

confidence: 99%

Advances in Aptamer-Based Biosensors for Food Safety

McKeague¹,

Giamberardino²,

DeRosa³

2011

Environmental Biosensors

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“…Comparing with the other aptamer-based methods for OTA detection [24][25][26][27][28][29][30][31][32][33][34][35][36][37][38], the sensitivity of our strategy is better than that of the methods using gold nanoparticles (limit of detection (LOD), 22 nM) [33], graphenes (1.9 μM) [29], carbon nanotubes (LOD, 24.5 nM) [38], and fluorescence ploarization (LOD, 5 nM) [22], but lower than that of some reported electrochemical methods [30], which allowed the detection of OTA at concentration lower than 1 nM. Some research groups recently reported the LOD of OTA reached 0.5 [27] or 0.25 pM [28] by using signal amplification strategy or nanomaterials in the aptamer-based assays.…”

Section: Screening the Labeling Sitesmentioning

confidence: 99%

“…The DNA aptamer for OTA has a high binding affinity, and the dissociation constant reaches about 50 nM [21,23]. Many aptamer-based methods for OTA analysis recently have been reported [24][25][26][27][28][29][30][31][32][33][34][35][36][37][38]. These methods have involved colorimetric, electrochemical, fluorescent, and chromatographic approaches.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent sensing ochratoxin A with single fluorophore-labeled aptamer

2013

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We explored a fluorescent strategy for sensing ochratoxin A (OTA) by using a single fluorophore-labeled aptamer for detection of OTA. This method relied on the change of the fluorescence intensity of the labeled dye induced by the specific binding of the fluorescent aptamer to OTA. Different fluorescein labeling sites of aptamers were screened, including the internal thymine bases, 3′-end, and 5′-end of the aptamer, and the effect of the labeling on the aptamer affinity was investigated. Some fluorophorelabeled aptamers showed a signal-on or signal-off response. With the fluorescent aptamer switch, simple, rapid, and selective sensing of OTA at nanomolar concentrations was achieved. OTA spiked in diluted red wine could be detected, showing the feasibility of the fluorescent aptamer for a complex matrix. This method shows potential for designing aptamer sensors for other targets.

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“…Since the report of an aptamer for ochratoxin A (OTA) in 2008, 22 many aptasensors for OTA and AFB 1 have been developed for feed and food safety. [23][24][25][26][27][28][29] In our previous study, 27 an aptasensor based on qPCR was successfully designed for AFB 1 determination with high sensitivity. Aptasensors were developed for the detection of AFM 1 using electrochemical methods and impedance spectroscopy techniques.…”

Section: Introductionmentioning

confidence: 99%

A qPCR aptasensor for sensitive detection of aflatoxin M1

et al. 2016

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. In addition, the aptasensor developed here exhibits high selectivity for AFM 1 over other mycotoxins and small effects from cross-reaction with structural analogs. The method proposed here has been successfully applied to quantitative determination of AFM 1 in infant rice cereal and infant milk powder samples. Results demonstrated that the current approach is potentially useful for food safety analysis, and it could be extended to a large number of targets. IntroductionAflatoxin M 1 (AFM 1 ), one of the most toxic contaminants in dairy products, is a metabolite produced by dairy cows as a result of being fed with feeds contaminated with Aflatoxin B 1 (AFB 1 ). [3][4][5] Once present in dairy products, AFM 1 poses a hazard to humans (especially infants) who consume them. Results and Discussion Optimization of the amplification of complementary ssDNAThe complementary ssDNA is applied as the subsequent PCR template. The Optimization of streptavidin and the biotinylated aptamerOther factors would affect the performance of this method including the concentrations of streptavidin, the biotinylated aptamer and the complementary ssDNA and these all should be optimized. Under a fixed concentration of complementary ssDNA at 10 nM, the concentrations of streptavidin and the 6 biotinylated aptamer were analyzed for the PCR amplification signal change (Fig. S3).The adsorptive power of streptavidin to PCR tubes and the binding ability of streptavidin-coated tubes to biotinylated aptamer was primarily detected using different concentrations of streptavidin (Fig. S3). This also shows that there was a clear difference of Ct values between the control group and streptavidin-coated tubes, which showed the intense adsorptive ability between biotinylated aptamer and streptavidin-coated tubes. In analysis of the Ct values at different concentrations of streptavidin, the Ct value reached the lowest level when the concentration of streptavidin was 2.5 ng mL -1 . This graph also shows that the Ct values decreased with the increase of the amount of aptamer when aptamer levels were below 10.0 nM andalso that the Ct values increased with the amount of aptamer above 10.0 nM mainly because of steric hindrance. Thus, 2.5 ng mL -1 of streptavidin and 10 nM of aptamer were the optimal conditions for RT-qPCR amplification. AFM 1 determinationUnder optimal conditions, the amplification curves and calibration curve of this aptasensor for different levels of AFM 1 with AFM 1 DNA1 were determined by RT-qPCR (Fig. 1). As is shown in Fig. 1(A), the cycle number increased with an increase in AFM 1 . More complementary ssDNA would be released when more AFM 1 is present in the reaction system, which leads to a decrease in the amount of the PCR template and an increase in cycle number. Meanwhile, a good linear relationshipbetween Ct values and AFM 1 levels in the range of 1×10 -4 to 1 µg L -1 was obtained as (Table 1). Specificity analysisThe specificity of the aptasensor plays an important role in the development and practicality of this method. In ...

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Fabricated aptamer-based electrochemical “signal-off” sensor of ochratoxin A

Cited by 203 publications

References 46 publications

Advances in Aptamer-Based Biosensors for Food Safety

Advances in Aptamer-Based Biosensors for Food Safety

Fluorescent sensing ochratoxin A with single fluorophore-labeled aptamer

A qPCR aptasensor for sensitive detection of aflatoxin M1

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