F18ab Escherichia coli flagella expression is regulated by acyl-homoserine lactone and contributes to bacterial virulence

Yang, Yang; Yao, Fenghua; Zhu, Guoqiang; Zhu, Jun; Zhang, Xinjun; Bao, Wenbin; Wu, Shenglong; Hardwidge, Philip R.; Zhu, Guoqiang

doi:10.1016/j.vetmic.2013.04.020

Cited by 21 publications

(31 citation statements)

References 35 publications

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“…When Shiga toxin-and verotoxin-producing E. coli were transformed with the yenI gene of Yersinia enterocolitica to produce AHLs, the transgenic strain showed impaired expression of flagella, decreased motility, reduced biofilm formation etc., as well as attenuated adherence and invasion of IPEC-12 cells (Yang et al 2013). Also, organic extracts obtained from aerobic granules from membrane-partitioned bioreactors used for wastewater treatments promote adhesion and growth of E. coli, and such activity has been attributed to the presence of AHLs in the liquid phase of granular sludge (Ren et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Aii20J, a wide-spectrum thermostable N-acylhomoserine lactonase from the marine bacterium Tenacibaculum sp. 20J, can quench AHL-mediated acid resistance in Escherichia coli

Mayer

Romero

Muras

et al. 2015

Appl Microbiol Biotechnol

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Acyl homoserine lactones (AHLs) are produced by many Gram-negative bacteria to coordinate gene expression in cellular density dependent mechanisms known as quorum sensing (QS). Since the disruption of the communication systems significantly reduces virulence, the inhibition of quorumsensing processes or quorum quenching (QQ) represents an interesting anti-pathogenic strategy to control bacterial infections. Escherichia coli does not produce AHLs but possesses an orphan AHL receptor, SdiA, which is thought to be able to sense the QS signals produced by other bacteria and controls important traits as the expression of glutamate-dependent acid resistance mechanism, therefore constituting a putative target for QQ. A novel AHL-lactonase, named Aii20J, has been identified, cloned and over expressed from the marine bacterium Tenacibaculum sp. strain 20 J presenting a wide-spectrum QQ activity. The enzyme, belonging to the metallo-β-lactamase family, shares less than 31 % identity with the lactonase AiiA from Bacillus spp. Aii20J presents a much higher specific activity than the Bacillus enzyme, maintains its activity after incubation at 100 ºC for 10 minutes, is resistant to protease K and α-chymotrypsin, and is unaffected by wide ranges of pH. The addition of Aii20J (20 μg/mL) to cultures of E. coli K-12 to which OC6-HSL was added resulted in a significant reduction in cell viability in comparison with the acidresistant cultures derived from the presence of the signal. Results confirm the interaction between AHLs and SdiA in E. coli for the expression of virulence-related genes and reveal the potential use of Aii20J as anti-virulence strategy against important bacterial pathogens and in other biotechnological applications.

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Section: Discussionmentioning

confidence: 99%

Aii20J, a wide-spectrum thermostable N-acylhomoserine lactonase from the marine bacterium Tenacibaculum sp. 20J, can quench AHL-mediated acid resistance in Escherichia coli

Mayer

Romero

Muras

et al. 2015

Appl Microbiol Biotechnol

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“…The C4-HSL production by P. aeruginosa strains was screened using a plate-based bioassay that was modified from a previously published protocol [53,57,58]. Briefly, E. coli XL-1 harbouring rhlA-lacZ reporter was used for the detection of butyryl-HSL in the X-gal substrate conversion assay [53].…”

Section: C4 Homoserine-lactone (Hsl) Reporter Bioassaymentioning

confidence: 99%

Pseudomonas aeruginosa biofilm formation on endotracheal tubes requires multiple two-component systems

Badal

Jayarani

Kollaran

et al. 2020

Journal of Medical Microbiology

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Introduction. Indwelling medical devices such as endotracheal tubes (ETTs), urinary catheters, vascular access devices, tracheostomies and feeding tubes are often associated with hospital-acquired infections. Bacterial biofilm formed on the ETTs in intubated patients is a significant risk factor associated with ventilator-associated pneumonia. Pseudomonas aeruginosa is one of the four frequently encountered bacteria responsible for causing pneumonia, and the biofilm formation on ETTs. However, understanding of biofilm formation on ETT and interventions to prevent biofilm remains lagging. The ability to sense and adapt to external cues contributes to their success. Thus, the biofilm formation is likely to be influenced by the two-component systems (TCSs) that are composed of a membrane-associated sensor kinase and an intracellular response regulator. Aim. This study aims to establish an in vitro method to analyse the P. aeruginosa biofilm formation on ETTs, and identify the TCSs that contribute to this process. Methodology. In total, 112 P. aeruginosa PA14 TCS mutants were tested for their ability to form biofilm on ETTs, their effect on quorum sensing (QS) and motility. Results. Out of 112 TCS mutants studied, 56 had altered biofilm biomass on ETTs. Although the biofilm formation on ETTs is QS-dependent, none of the 56 loci controlled quorum signal. Of these, 18 novel TCSs specific to ETT biofilm were identified, namely, AauS, AgtS, ColR, CopS, CprR, NasT, KdpD, ParS, PmrB, PprA, PvrS, RcsC, PA14_11120, PA14_32580, PA14_45880, PA14_49420, PA14_52240, PA14_70790. The set of 56 included the GacS network, TCS proteins involved in fimbriae synthesis, TCS proteins involved in antimicrobial peptide resistance, and surface-sensing. Additionally, several of the TCS-encoding genes involved in biofilm formation on ETTs were found to be linked to flagellum-dependent swimming motility. Conclusions. Our study established an in vitro method for studying P. aeruginosa biofilm formation on the ETT surfaces. We also identified novel ETT-specific TCSs that could serve as targets to prevent biofilm formation on indwelling devices frequently used in clinical settings.

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“…In addition to the fimbriae-specific regulators, each fimbrial gene cluster typically belongs to specific regulons that can be activated or repressed by global regulators such as H-NS (histone-like nucleoid structuring protein for temperature and osmolarity mediated signals) or CRP (cAMP receptor protein for catabolite repression). Some intriguing data highlighted the possibility that auto-inducer molecules expressed by the intestinal microbiota or host catecholamines have an effect on intestinal colonization by ETEC (263). Recent data indicated increased gene expression and production of F4 fimbriae by an ETEC strain grown in the presence of conditioned media and epinephrine (264), but more work is needed to dissect the corresponding mechanisms of regulation.…”

Section: Regulation Of Fimbrial Expressionmentioning

confidence: 99%

Animal EnterotoxigenicEscherichia coli

2016

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Enterotoxigenic Escherichia coli (ETEC) is the most common cause of E. coli diarrhea in farm animals. ETEC are characterized by the ability to produce two types of virulence factors; adhesins that promote binding to specific enterocyte receptors for intestinal colonization and enterotoxins responsible for fluid secretion. The best-characterized adhesins are expressed in the context of fimbriae, such as the F4 (also designated K88), F5 (K99), F6 (987P), F17 and F18 fimbriae. Once established in the animal small intestine, ETEC produces enterotoxin(s) that lead to diarrhea. The enterotoxins belong to two major classes; heat-labile toxin that consist of one active and five binding subunits (LT), and heat-stable toxins that are small polypeptides (STa, STb, and EAST1). This chapter describes the disease and pathogenesis of animal ETEC, the corresponding virulence genes and protein products of these bacteria, their regulation and targets in animal hosts, as well as mechanisms of action. Furthermore, vaccines, inhibitors, probiotics and the identification of potential new targets identified by genomics are presented in the context of animal ETEC.

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F18ab Escherichia coli flagella expression is regulated by acyl-homoserine lactone and contributes to bacterial virulence

Cited by 21 publications

References 35 publications

Aii20J, a wide-spectrum thermostable N-acylhomoserine lactonase from the marine bacterium Tenacibaculum sp. 20J, can quench AHL-mediated acid resistance in Escherichia coli

Aii20J, a wide-spectrum thermostable N-acylhomoserine lactonase from the marine bacterium Tenacibaculum sp. 20J, can quench AHL-mediated acid resistance in Escherichia coli

Pseudomonas aeruginosa biofilm formation on endotracheal tubes requires multiple two-component systems

Animal EnterotoxigenicEscherichia coli

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