F-box only and CUE proteins are crucial ubiquitination-associated components for conidiation and pathogenicity in the rice blast fungus, Magnaporthe oryzae

Lim, You-Jin; Lee, Yong-Hwan

doi:10.1016/j.fgb.2020.103473

Cited by 12 publications

(10 citation statements)

References 77 publications

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“…MoGrr1 is another F-box protein that participates in conidiogenesis, maintenance of cell wall integrity and viral pathogenicity of M. oryzae [27]. Some other F-box proteins have also been reported to be involved in the conidiation and pathogenicity of M. oryzae [28]. The grrA gene encodes a protein similar to fungal F-box proteins, indicated by ΔgrrA mutants that are unable to form mature ascospores [23].…”

Section: Discussionmentioning

confidence: 99%

A putative F‐box‐domain‐encoding gene AOL_s00076g207 regulates the development and pathogenicity of Arthrobotrys oligospora

Peng

Dong

et al. 2021

J Basic Microbiol

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F-box protein is a key component of the Skp1-cullin-F-box-type ubiquitin ligase complex (SCF-ULC) that marks its target proteins with ubiquitin for proteasomal degradation. In this study, we explored the potential role of AOL_s00076g207 (Aog207) in Arthrobotrys oligospora, a model fungus for studying nematodes-fungi interactions. The Aog207 gene encodes a putative F-box protein of the SCF-ULC. Deletion of Aog207 could inhibit mycelial growth in TYGA and PDA media. More importantly, the conidial germination rate of ΔAog207 mutants was remarkably declined compared to that of wildtype (WT) strain, and the mutant strains were more sensitive toward chemical stressors than the WT strain. In addition, ΔAog207 mutants generated fewer traps and captured fewer nematodes than WT strain. In summary, Aog207 disruption significantly affected the pathogenicity, mycelial growth, conidial germination, environmental adaptation and trap formation of A. oligospora.These findings may facilitate a better understanding of the nematode predation mechanism of A. oligospora and provide an experimental basis for developing biological control agents against nematodes.

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Section: Discussionmentioning

confidence: 99%

A putative F‐box‐domain‐encoding gene AOL_s00076g207 regulates the development and pathogenicity of Arthrobotrys oligospora

Peng

Dong

et al. 2021

J Basic Microbiol

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“…MoFwd1 is also involved in circadian rhythm through modifying the transcription and protein stability of MoFrq, a core circadian regulator (Shi et al 2019). Recent studies showed that the ubiquitination level of total protein in ΔMofbx15 is decreased compared with that of wild type, which further proved that SCF ubiquitin ligases containing F-box proteins play indispensable roles in the development and pathogenicity of M. oryzae by affecting protein ubiquitination (Lim and Lee 2020).…”

Section: E3 Ligasementioning

confidence: 94%

Ubiquitination in the rice blast fungus Magnaporthe oryzae: from development and pathogenicity to stress responses

et al. 2022

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Ubiquitination is a vital protein post-translational modification (PTM) prevalent in eukaryotes. This modification regulates multiple cellular processes through protein degradation mediated by the 26S proteasome or affecting protein–protein interaction and protein localization. Magnaporthe oryzae causes rice blast disease, which is one of the most devastating crop diseases worldwide. In M. oryzae, ubiquitination plays important roles in growth, pathogenicity, stress response and effector-mediated plant-pathogen interaction. In this review, we summarize the roles of ubiquitination components in the above biological processes of M. oryzae, including single- or multi-subunit E3s, E2s, components of 26S proteasome and also deubiquitinating enzymes. The essential function of ubiquitination in plant-fungus interaction is also discussed. Moreover, this review presents several issues related to the ubiquitination system in M. oryzae, which need to be further explored in future researches.

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“…Another F-box protein, Cdc4, has also been studied in S. cerevisiae (Feldman et al, 1997;Orlicky et al, 2003) and C. albicans (Atir-Lande et al, 2005;Shieh et al, 2005), which have also been shown to be associated with the cell cycle and morphological development of fungi. The involvement of the F-box proteins in the virulence of plant-pathogenic fungi has also been reported recently (Duyvesteijn et al, 2005;Han et al, 2007;Shi et al, 2019;Lim and Lee, 2020). So far, the role of F-box protein in the virulence of human fungal pathogens is rarely reported.…”

Section: Introductionmentioning

confidence: 92%

“…Our results further showed that Cdc4 is critical for fungal virulence and sexual reproduction in C. neoformans, similar to the function of another F-box protein, Fbp1, identified by us previously. Since F-box proteins have also been reported to play roles in fungal infectivity in plant-pathogenic fungi (Duyvesteijn et al, 2005;Han et al, 2007;Shi et al, 2019;Lim and Lee, 2020)., the involvement of F-box proteins in virulence is likely conserved in fungal pathogens. Cryptococcus Cdc4 shares sequence and structural similarity with Cdc4 in S. cerevisiae, C. albicans, and A. fumigatus (Figures 1A, B).…”

Section: Disscussionmentioning

confidence: 99%

Role of F-box Protein Cdc4 in Fungal Virulence and Sexual Reproduction of Cryptococcus neoformans

Fan

Han

et al. 2022

Front. Cell. Infect. Microbiol.

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Cryptococcus neoformans is an opportunistic yeast-like pathogen that mainly infects immunocompromised individuals and causes fatal meningitis. Sexual reproduction can promote the exchange of genetic material between different strains of C. neoformans, which is one of the reasons leading to the emergence of highly pathogenic and drug-resistant strains of C. neoformans. Although much research has been done on the regulation mechanism of Cryptococcus sexual reproduction, there are few studies on the sexual reproduction regulation of Cryptococcus by the ubiquitin-proteasome system. This study identified an F-box protein, Cdc4, which contains a putative F-box domain and eight WD40 domains. The expression pattern analysis showed that the CDC4 gene was expressed in various developmental stages of C. neoformans, and the Cdc4 protein was localized in the nucleus of cryptococcal cells. In vitro stress responses assays showed that the CDC4 overexpression strains are sensitive to SDS and MMS but not Congo red, implying that Cdc4 may regulate the cell membrane integrity and repair of DNA damage of C. neoformans. Fungal virulence assay showed that although the cdc4Δ mutant grows normally and can produce typical virulence factors such as capsule and melanin, the cdc4Δ mutant completely loses its pathogenicity in a mouse systemic-infection model. Fungal mating assays showed that Cdc4 is also essential for fungal sexual reproduction in C. neoformans. Although normal mating hyphae were observed during mating, the basidiospores’ production was blocked in bilateral mating between cdc4Δ mutants. Fungal nuclei development assay showed that the nuclei failed to undergo meiosis after fusion inside the basidia during the bilateral mating of cdc4Δ mutants, indicating that Cdc4 is critical to regulating meiosis during cryptococcal mating. In summary, our study revealed that the F-box protein Cdc4 is critical for fungal virulence and sexual reproduction in C. neoformans.

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F-box only and CUE proteins are crucial ubiquitination-associated components for conidiation and pathogenicity in the rice blast fungus, Magnaporthe oryzae

Cited by 12 publications

References 77 publications

A putative F‐box‐domain‐encoding gene AOL_s00076g207 regulates the development and pathogenicity of Arthrobotrys oligospora

A putative F‐box‐domain‐encoding gene AOL_s00076g207 regulates the development and pathogenicity of Arthrobotrys oligospora

Ubiquitination in the rice blast fungus Magnaporthe oryzae: from development and pathogenicity to stress responses

Role of F-box Protein Cdc4 in Fungal Virulence and Sexual Reproduction of Cryptococcus neoformans

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