F-BOX and oleosin: additional target genes for future metabolic engineering in tung trees?

Chen, Yicun; Zhou, Guan; Wang, Yangdong; Xu, Lingna

doi:10.1016/j.indcrop.2010.06.009

Cited by 15 publications

(15 citation statements)

References 9 publications

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“…OLE3 and OLE5 are identical to those isolated from cDNA library of tung seed EST (vf1-GR217754 and vf5-GR218198) [35]. The reported vf4 has an N-terminal extension and only six varied amino acid residues at the C-terminus of the protein compared to vf3 and clustered together in the phylogenetic tree [35], suggesting that these two genes constitute a paralogous pair, a common occurrence in eudicot species such as Arabidopsis [71]. It might be preferable to name the reported vf3 and vf4 as OLE3a and OLE3b, respectively.…”

Section: Discussionmentioning

confidence: 83%

Identification, Classification and Differential Expression of Oleosin Genes in Tung Tree (Vernicia fordii)

et al. 2014

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Triacylglycerols (TAG) are the major molecules of energy storage in eukaryotes. TAG are packed in subcellular structures called oil bodies or lipid droplets. Oleosins (OLE) are the major proteins in plant oil bodies. Multiple isoforms of OLE are present in plants such as tung tree (Vernicia fordii), whose seeds are rich in novel TAG with a wide range of industrial applications. The objectives of this study were to identify OLE genes, classify OLE proteins and analyze OLE gene expression in tung trees. We identified five tung tree OLE genes coding for small hydrophobic proteins. Genome-wide phylogenetic analysis and multiple sequence alignment demonstrated that the five tung OLE genes represented the five OLE subfamilies and all contained the “proline knot” motif (PX5SPX3P) shared among 65 OLE from 19 tree species, including the sequenced genomes of Prunus persica (peach), Populus trichocarpa (poplar), Ricinus communis (castor bean), Theobroma cacao (cacao) and Vitis vinifera (grapevine). Tung OLE1, OLE2 and OLE3 belong to the S type and OLE4 and OLE5 belong to the SM type of Arabidopsis OLE. TaqMan and SYBR Green qPCR methods were used to study the differential expression of OLE genes in tung tree tissues. Expression results demonstrated that 1) All five OLE genes were expressed in developing tung seeds, leaves and flowers; 2) OLE mRNA levels were much higher in seeds than leaves or flowers; 3) OLE1, OLE2 and OLE3 genes were expressed in tung seeds at much higher levels than OLE4 and OLE5 genes; 4) OLE mRNA levels rapidly increased during seed development; and 5) OLE gene expression was well-coordinated with tung oil accumulation in the seeds. These results suggest that tung OLE genes 1–3 probably play major roles in tung oil accumulation and/or oil body development. Therefore, they might be preferred targets for tung oil engineering in transgenic plants.

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Section: Discussionmentioning

confidence: 83%

Identification, Classification and Differential Expression of Oleosin Genes in Tung Tree (Vernicia fordii)

et al. 2014

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“…These genes code for diacylglycerol acyltransferase 1 and 2 (DGAT1 and DGAT2) [16], delta-12 oleic acid desaturase (FAD2) [17], delta-12 fatty acid conjugase (FADX) [17], omega-3 fatty acid desaturase (FAD3) [18], acyl-CoA binding proteins (ACBPs) [19], cytochrome b5 isoforms (Cb5s) [20], cytochrome b5 reductase [21], glycerol-3-phosphate acyltransferases (GPATs) [22], aquaporin [23], glutaredoxin [23] and oleosins [24].…”

Section: Introductionmentioning

confidence: 99%

“…The DGAT1s and DGAT2s have similar properties and amino acid composition except that DGAT1s are approximately 20 kDa larger than DGAT2s [33]. DGAT isoforms have non-redundant functions in TAG biosynthesis in species such as mice [34] and tung tree [16], [24].…”

Section: Introductionmentioning

confidence: 99%

Developmental Regulation of Diacylglycerol Acyltransferase Family Gene Expression in Tung Tree Tissues

et al. 2013

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Diacylglycerol acyltransferases (DGAT) catalyze the final and rate-limiting step of triacylglycerol (TAG) biosynthesis in eukaryotic organisms. DGAT genes have been identified in numerous organisms. Multiple isoforms of DGAT are present in eukaryotes. We previously cloned DGAT1 and DGAT2 genes of tung tree (Vernicia fordii), whose novel seed TAGs are useful in a wide range of industrial applications. The objective of this study was to understand the developmental regulation of DGAT family gene expression in tung tree. To this end, we first cloned a tung tree gene encoding DGAT3, a putatively soluble form of DGAT that possesses 11 completely conserved amino acid residues shared among 27 DGAT3s from 19 plant species. Unlike DGAT1 and DGAT2 subfamilies, DGAT3 is absent from animals. We then used TaqMan and SYBR Green quantitative real-time PCR, along with northern and western blotting, to study the expression patterns of the three DGAT genes in tung tree tissues. Expression results demonstrate that 1) all three isoforms of DGAT genes are expressed in developing seeds, leaves and flowers; 2) DGAT2 is the major DGAT mRNA in tung seeds, whose expression profile is well-coordinated with the oil profile in developing tung seeds; and 3) DGAT3 is the major form of DGAT mRNA in tung leaves, flowers and immature seeds prior to active tung oil biosynthesis. These results suggest that DGAT2 is probably the major TAG biosynthetic isoform in tung seeds and that DGAT3 gene likely plays a significant role in TAG metabolism in other tissues. Therefore, DGAT2 should be a primary target for tung oil engineering in transgenic organisms.

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“…Since then, various forms of tree OLEs had been identified in Elaeis guineensis (oil palm) (Yong et al, 1999), Theobroma cacao (cacao) (Guilloteau et al, 2003), Ricinus communis (castor bean) (Eastmond, 2004), Prunus armeniaca (apricot) (Geuna et al, 2005), Coffea arabica (coffee) (Simkin et al, 2006), Coffea canephora (coffee) (Simkin et al, 2006), Corylus avellana (hazelnut) (Akkerdaas et al, 2006), Camellia oleifera (tea oil), Olea europaea (olive) (Giannoulia et al, 2007), Persea americana (avocado) (Lopez-Gomez et al, 2007), Cocos nucifera (coconut palm) (Regalado et al, 2008), Ficus pumila (climbing fig) (Chua et al, 2008), Cupressus sempervirens (pencil pine) (Pedron et al, 2009), Pinus taeda (loblolly pine) (Kovach et al, 2010), Vernicia fordii (tung tree) Chen et al, 2010a;Long et al, 2010), Jatropha curcas (barbados nut) (Gu et al, 2012;Popluechai et al, 2011;Xu et al, 2011), and Juglans regia (walnut).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of Oleosin Gene Family in 22 Tree Species: An Accelerator for Metabolic Engineering of BioFuel Crops and Agrigenomics Industrial Applications?

Cao

2015

OMICS: A Journal of Integrative Biology

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Trees contribute to enormous plant oil reserves because many trees contain 50%-80% of oil (triacylglycerols, TAGs) in the fruits and kernels. TAGs accumulate in subcellular structures called oil bodies/droplets, in which TAGs are covered by low-molecular-mass hydrophobic proteins called oleosins (OLEs). The OLEs/TAGs ratio determines the size and shape of intracellular oil bodies. There is a lack of comprehensive sequence analysis and structural information of OLEs among diverse trees. The objectives of this study were to identify OLEs from 22 tree species (e.g., tung tree, tea-oil tree, castor bean), perform genome-wide analysis of OLEs, classify OLEs, identify conserved sequence motifs and amino acid residues, and predict secondary and threedimensional structures in tree OLEs and OLE subfamilies. Data mining identified 65 OLEs with perfect conservation of the ''proline knot'' motif (PX5SPX3P) from 19 trees. These OLEs contained >40% hydrophobic amino acid residues. They displayed similar properties and amino acid composition. Genome-wide phylogenetic analysis and multiple sequence alignment demonstrated that these proteins could be classified into five OLE subfamilies. There were distinct patterns of sequence conservation among the OLE subfamilies and within individual tree species. Computational modeling indicated that OLEs were composed of at least three ahelixes connected with short coils without any b-strand and that they exhibited distinct 3D structures and ligand binding sites. These analyses provide fundamental information in the similarity and specificity of diverse OLE isoforms within the same subfamily and among the different species, which should facilitate studying the structurefunction relationship and identify critical amino acid residues in OLEs for metabolic engineering of tree TAGs.

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F-BOX and oleosin: additional target genes for future metabolic engineering in tung trees?

Cited by 15 publications

References 9 publications

Identification, Classification and Differential Expression of Oleosin Genes in Tung Tree (Vernicia fordii)

Identification, Classification and Differential Expression of Oleosin Genes in Tung Tree (Vernicia fordii)

Developmental Regulation of Diacylglycerol Acyltransferase Family Gene Expression in Tung Tree Tissues

Genome-Wide Analysis of Oleosin Gene Family in 22 Tree Species: An Accelerator for Metabolic Engineering of BioFuel Crops and Agrigenomics Industrial Applications?

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