EZH2 promotes gastric cancer cells proliferation by repressing p21 expression

Xu, Jiewei; Wang, Zhong; Lu, Wei; Jiang, Hao; Lu, Jun; Jian, Qiu; Ye, Guochao

doi:10.1016/j.prp.2019.03.003

Cited by 46 publications

(41 citation statements)

References 29 publications

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“…Previous studies have indicated that eZH2 enhances the proliferative capacity of gastric cancer cells by suppressing cyclin-dependent kinase inhibitor 1 (p21) expression (30,31). Furthermore, in laryngeal carcinoma, eZH2 promotes cell proliferation by regulating runt-related transcription factor 3 (runX3) (32).…”

Section: Discussionmentioning

confidence: 99%

Effects of miR‑195‑5p on cell proliferation and apoptosis in gestational diabetes mellitus via targeting EZH2

2020

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Gestational diabetes mellitus (GdM) is a type of diabetes mellitus (dM) that occurs during pregnancy. The present study aimed to investigate the roles of microrna (mir)-195-5p and enhancer of zeste homolog 2 (eZH2) in GdM, and their potential association. Human umbilical vein endothelial cells (HuVecs) were collected from healthy and GdM umbilical cords, and the endothelial properties were detected by flow cytometry. mRNA expression levels of miR-195-5p and EZH2, and EZH2 protein expression levels were detected by reverse transcription-quantitative Pcr (rT-qPcr) and western blot analysis, respectively. cell colony formation and flow cytometry were performed to determine cell proliferation and apoptosis. Furthermore, the target gene of mir-195-5p was predicted and assessed using a dual-luciferase reporter assay. The levels of cell viability, proliferation and apoptosis following the overexpression of miR-195-5p, EZH2 or mir-195-5p + eZH2, were detected using cell counting Kit-8, colony formation and flow cytometry assays, respectively. In addition, the mRNA expression levels of miR-195-59 and EZH2, and EZH2 protein expression levels following transfection with overexpression plasmids were detected using rT-qPcr and western blot analysis, respectively. it was identified that high mRNA expression of miR-195-5p, and low EZH2 mRNA and protein expression levels decreased the level of cell proliferation and the high apoptotic rate of GdM-HuVecs. in addition, mir-195-5p was predicted and identified to target EZH2, and miR-195-5p overexpression was identified to inhibit cell proliferation and promote apoptosis. However, it was demonstrated that upregulation of eZH2 could alleviate the inhibition of cell proliferation and the increased apoptotic rate induced by miR-195-5p overexpression. Therefore, the present results suggested that mir-195-5p may inhibit cell viability, proliferation and promote apoptosis by targeting eZH2 in GdM-induced HuVecs.

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Section: Discussionmentioning

confidence: 99%

Effects of miR‑195‑5p on cell proliferation and apoptosis in gestational diabetes mellitus via targeting EZH2

2020

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“…EZH2 dysregulation is a hallmark of disease progression in a number of different types of cancer. For example, in gastric cancer tissues, EZH2 expression was abnormally enhanced and EZH2 accelerated cell proliferation by targeting p21 (16). Lui et al (17) reported that EZH2 was a regulator of chondrocyte proliferation and hypertrophy; however, the relationship between miR-17-5p and EZH2 has not been reported.…”

Section: Introductionmentioning

confidence: 99%

miR‑17‑5p and miR‑19b‑3p prevent osteoarthritis progression by targeting EZH2

Yuan

Song

et al. 2020

Exp Ther Med

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Osteoarthritis (OA) is a joint disease caused by a variety of factors, including aging, obesity and trauma. MicroRNAs (miRNAs) have been reported to be crucial regulators during OA progression. The present study aimed to investigate the role of miR-17-5p and miR-19b-3p during OA development. Interleukin (IL)-1β-treated chondrocytes were used to mimic OA in vitro. The expression levels of miR-17-5p and enhancer of zeste homolog 2 (EZH2) were measured in cartilage tissues and chondrocytes using reverse transcription-quantitative PCR or western blotting. Apoptosis was assessed by flow cytometry. The protein expression levels of extracellular matrix (ECM)-associated genes were detected by western blotting. The binding sites between miR-17-5p or miR-19b-3p and EZH2 were predicted using the MicroT-CDS online database and verified using dual-luciferase reporter and RIP assays. miR-17-5p expression was downregulated, whereas EZH2 expression was upregulated in OA cartilage tissues and IL-1β-induced chondrocytes compared with that in the control tissues and cells. miR-17-5p mimics inhibited IL-1β-induced apoptosis and ECM degradation in chondrocytes. EZH2 was the target of miR-17-5p and miR-19b-3p in chondrocytes, and enhanced apoptosis and ECM degradation in IL-1β-stimulated chondrocytes. Rescue experiments revealed that miR-17-5p or miR-19b-3p mimic-induced inhibition of OA progression was reversed by EZH2 overexpression. In conclusion, miR-17-5p and miR-19b-3p inhibited OA progression by targeting EZH2, which may serve as a potential therapeutic target for OA.

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“…Previous studies have shown that p21 is over expressed in lung cancer and gastrointestinal cancer cells [49,50].The results of Zhang's study show that the 5-year survival rate of OSCC patients with high p21 expression is signi cantly reduced. Kaplan-Meier analysis and Cox proportional hazard model showed that lymph node metastasis and p21 expression were independent prognostic factors of OSCC [51], which are consistent with our ndings that silenced the hsa_circ_0127523, the TRIP13 expression level was decreases and the Notch1 decreases in the Notch pathway.…”

Section: Discussionmentioning

confidence: 92%

Regulation of Abnormal Expression of hsa_circRNA_0127523 on Proliferation, Migration and Invasion and mRNA Expression of miR-515-5p in Oral Squamous Cell Carcinoma

Wei

Wang

Gao

et al. 2020

Preprint

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Background: Oral squamous cell carcinoma (OSCC) is one of the most common cancer. Circular RNA (circRNA) play an important role in many biological processes of occurrence and development of cancers. However, the mechanism of circRNA in OSCC remains unclear. We successfully established a Chinese hamster oral squamous cell cancer animal model and discovered the differential expression of non-coding RNAs. In this study, we investigated the functional roles of a significantly up-regulated circRNA (hsa_circ_0127523) in OSCC. Methods: DMBA-induced malignant-transformed buccal sac tissue and normal tissue of Chinese hamster were analyzed by the second-generation circRNA sequencing. The top 8 different expression circRNAs in sequencing results were further confirmed by qRT-PCR. The RNAi, CCK-8, transwell migration and invasion assays were conducted to investigate the behavioral functions of hsa_circ_0127523 in OSCC cells. Results: In this study, we found total of 3485 circRNAs were differentially expressed. GO and KEGG analyses showed that differently expressed circRNAs function and pathway. The axis relationship of circRNA-miRNA was predicted by miRanda. Silencing hsa_circ_0127523 significantly suppressed the proliferation, migration and invasion abilities of OSCC cells. Furthermore, bioinformatics databases suggested that hsa_circ_0127523 might function by sponging miR-515-5p to regulate the expression of TRIP13. It was subsequently identified that hsa_circ_0127523 may inhibit miR-515-5p expression and increase TRIP13 expression in the OSCC cells. Conclusions: In summary, the results of the present study revealed that OSCC tissues have abundant circRNAs and we firstly explore the role of the hsa_circ_0127523 in the behavior regulation of OSCC cells. Its demonstrating that hsa_circ_0127523 may be a potential biomarker and therapeutic target of OSCC.

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EZH2 promotes gastric cancer cells proliferation by repressing p21 expression

Cited by 46 publications

References 29 publications

Effects of miR‑195‑5p on cell proliferation and apoptosis in gestational diabetes mellitus via targeting EZH2

Effects of miR‑195‑5p on cell proliferation and apoptosis in gestational diabetes mellitus via targeting EZH2

miR‑17‑5p and miR‑19b‑3p prevent osteoarthritis progression by targeting EZH2

Regulation of Abnormal Expression of hsa_circRNA_0127523 on Proliferation, Migration and Invasion and mRNA Expression of miR-515-5p in Oral Squamous Cell Carcinoma

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EZH2 promotes gastric cancer cells proliferation by repressing p21 expression

Cited by 46 publications

References 29 publications

Effects of miR‑195‑5p on cell proliferation and apoptosis in gestational diabetes mellitus via targeting EZH2

Effects of miR‑195‑5p on cell proliferation and apoptosis in gestational diabetes mellitus via targeting EZH2

miR‑17‑5p and miR‑19b‑3p prevent osteoarthritis progression by targeting EZH2

Regulation of Abnormal Expression of hsa_circRNA_0127523&nbsp;on Proliferation, Migration and Invasion and mRNA&nbsp;Expression of miR-515-5p&nbsp;in Oral Squamous Cell Carcinoma

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Regulation of Abnormal Expression of hsa_circRNA_0127523 on Proliferation, Migration and Invasion and mRNA Expression of miR-515-5p in Oral Squamous Cell Carcinoma