Regulation of Abnormal Expression of hsa_circRNA_0127523 on Proliferation, Migration and Invasion and mRNA Expression of miR-515-5p in Oral Squamous Cell Carcinoma

Abstract

Background: Oral squamous cell carcinoma (OSCC) is one of the most common cancer. Circular RNA (circRNA) play an important role in many biological processes of occurrence and development of cancers. However, the mechanism of circRNA in OSCC remains unclear. We successfully established a Chinese hamster oral squamous cell cancer animal model and discovered the differential expression of non-coding RNAs. In this study, we investigated the functional roles of a significantly up-regulated circRNA (hsa_circ_0127523) in OSCC. Methods: DMBA-induced malignant-transformed buccal sac tissue and normal tissue of Chinese hamster were analyzed by the second-generation circRNA sequencing. The top 8 different expression circRNAs in sequencing results were further confirmed by qRT-PCR. The RNAi, CCK-8, transwell migration and invasion assays were conducted to investigate the behavioral functions of hsa_circ_0127523 in OSCC cells. Results: In this study, we found total of 3485 circRNAs were differentially expressed. GO and KEGG analyses showed that differently expressed circRNAs function and pathway. The axis relationship of circRNA-miRNA was predicted by miRanda. Silencing hsa_circ_0127523 significantly suppressed the proliferation, migration and invasion abilities of OSCC cells. Furthermore, bioinformatics databases suggested that hsa_circ_0127523 might function by sponging miR-515-5p to regulate the expression of TRIP13. It was subsequently identified that hsa_circ_0127523 may inhibit miR-515-5p expression and increase TRIP13 expression in the OSCC cells. Conclusions: In summary, the results of the present study revealed that OSCC tissues have abundant circRNAs and we firstly explore the role of the hsa_circ_0127523 in the behavior regulation of OSCC cells. Its demonstrating that hsa_circ_0127523 may be a potential biomarker and therapeutic target of OSCC.