Extremophilic microorganisms: Biochemical adaptation and biotechnological application (review)

Морозкина, Е. В.; Slutskaya, E. S.; Федорова, Т.В.; Tugay, Tatyana; Golubeva, L. I.; Королева, О. В.

doi:10.1134/s0003683810010011

Cited by 62 publications

(30 citation statements)

References 118 publications

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“…Elucidation of the integral biology of thermophilic and other extremophilic microorganisms for industrial applications has been greatly propagated in recent times [1,2]. Several research works on thermophilic anaerobes along with their enzymes are going on due to their huge biotechnological applications [3,4].…”

Section: Introductionmentioning

confidence: 99%

Participatory role of zinc in structural and functional characterization of bioremediase: a unique thermostable microbial silica leaching protein

et al. 2015

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A unique protein, bioremediase (UniProt Knowledgebase Accession No.: P86277), isolated from a hot spring bacterium BKH1 (GenBank Accession No.: FJ177512), has shown to exhibit silica leaching activity when incorporated to prepare bio-concrete material. Matrix-assisted laser desorption ionization mass spectrometry analysis suggests that bioremediase is 78% homologous to bovine carbonic anhydrase II though it does not exhibit carbonic anhydrase-like activity. Bioinformatics study is performed for understanding the various physical and chemical parameters of the protein which predicts the involvement of zinc encircled by three histidine residues (His94, His96 and His119) at the active site of the protein. Isothermal titration calorimetric-based thermodynamic study on diethyl pyrocarbonate-modified protein recognizes the presence of Zn(2+) in the enzyme moiety. Exothermic to endothermic transition as observed during titration of the protein with Zn(2+) discloses that there are at least two binding sites for zinc within the protein moiety. Addition of Zn(2+) regains the activity of EDTA chelated bioremediase confirming the presence of extra binding site of Zn(2+) in the protein moiety. Revival of folding pattern of completely unfolded urea-treated protein by Zn(2+) explains the participatory role of zinc in structural stability of the protein. Restoration of the λ max in intrinsic fluorescence emission study of the urea-treated protein by Zn(2+) similarly confirms the involvement of Zn in the refolding of the protein. The utility of bioremediase for silica nanoparticles preparation is observed by field emission scanning electron microscopy.

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Section: Introductionmentioning

confidence: 99%

Participatory role of zinc in structural and functional characterization of bioremediase: a unique thermostable microbial silica leaching protein

et al. 2015

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“…As for the caseinases, their industrial The industrial and household enzymes market has been growing continuously over growing in the last decades (Sarrouh et al 2012) and the search for novel, more stable and active enzymes and their respective producer microorganisms is of great interest. In this context, extremophiles are a source of special enzymes (Elleuche et al 2014;Morozkina et al 2010). The production of keratinase by a polyextremophile is an interesting characteristic for an industrial microorganism.…”

Section: Discussionmentioning

confidence: 99%

Extracellular peptidases from Deinococcus radiodurans

et al. 2015

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The extremophile Deinococcus radiodurans wild type R1 produces peptidases (metallo- and serine-) in TGY medium and in the media supplemented with human hair (HMY) and chicken feathers (FMY). Enzymatic screening on agar plates revealed peptidase activity. In TGY medium metallopeptidases were detected corresponding to a molecular mass range of 300-85 kDa (gelatinases); 280-130 (caseinases) and a 300 and a 170 kDa (keratinases); and a gelatinolytic serine peptidase (75 kDa). In HMY medium after 144 h, D. radiodurans produced keratinase (290 U/ml), gelatinase (619 U/ml) and sulfite (26 µg/ml). TGY medium produced higher proteolytic activity: 950 U/ml of gelatinolytic (24 h); 470 U/ml of keratinolytic (24 h) and 110 U/ml of caseinolytic (72 h). In the FMY medium, we found gelatinolytic (317 U/ml), keratinolytic (43 U/ml) and caseinolytic (85 U/ml) activities. The sulfite had a maximum release at 48 h (8.1 µg/ml). Enzymography analysis revealed that the keratinases degraded keratin after 24 h of reaction. The addition of sodium sulfite (1.0 %) improved the keratin degradation. Environmental Scanning Electron microscopy revealed alterations such as damage and holes in the hair fiber cuticle after D. radiodurans growth. This work presents for the first time D. radiodurans as a new keratinolytic microorganism.

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“…Acidophiles are microorganisms that grow optimally at pH values of 2.0 [35]. Acidic environments are especially interesting because, in general, the low pH of the habitat is the consequence of microbial metabolism, and not a condition imposed by the system, as is the case for other extreme environments.…”

Section: Acidophilesmentioning

confidence: 99%

Resistance of Microorganisms to Extreme Environmental Conditions and Its Contribution to Astrobiology

Rampelotto

2010

Sustainability

129

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Abstract:In the last decades, substantial changes have occurred regarding what scientists consider the limits of habitable environmental conditions. For every extreme environmental condition investigated, a variety of microorganisms have shown that not only can they tolerate these conditions, but that they also often require these extreme conditions for survival. Microbes can return to life even after hundreds of millions of years. Furthermore, a variety of studies demonstrate that microorganisms can survive under extreme conditions, such as ultracentrifugation, hypervelocity, shock pressure, high temperature variations, vacuums, and different ultraviolet and ionizing radiation intensities, which simulate the conditions that microbes could experience during the ejection from one planet, the journey through space, as well as the impact in another planet. With these discoveries, our knowledge about the biosphere has grown and the putative boundaries of life have expanded. The present work examines the recent discoveries and the principal advances concerning the resistance of microorganisms to extreme environmental conditions, and analyzes its contributions to the development of the main themes of astrobiology: the origins of life, the search for extraterrestrial life, and the dispersion of life in the Universe.

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Extremophilic microorganisms: Biochemical adaptation and biotechnological application (review)

Cited by 62 publications

References 118 publications

Participatory role of zinc in structural and functional characterization of bioremediase: a unique thermostable microbial silica leaching protein

Participatory role of zinc in structural and functional characterization of bioremediase: a unique thermostable microbial silica leaching protein

Extracellular peptidases from Deinococcus radiodurans

Resistance of Microorganisms to Extreme Environmental Conditions and Its Contribution to Astrobiology

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