Extremely low frequency magnetic field induced changes in motor behaviour of gerbils submitted to global cerebral ischemia

Rauš, Snežana; Selaković, Vesna; Radenović, Lidija; Prolić, Zlatko; Janać, Branka

doi:10.1016/j.bbr.2011.10.046

Cited by 21 publications

(21 citation statements)

References 45 publications

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“…At the behavioral level, motor functions were evaluated by neurological disability status scale, locomotor activity and inclined beam-walking test. Cerebral ischemia is documented to impair motor ability and provoke pronounced motor hyperactivity, which is most prominent on the first postischemic day after ischemic damage (Araki et al, 2001;Janać et al 2006;Kuroiwa et al 1991;Rauš et al, 2012). Our findings are in accordance with the already published data.…”

Section: Discussionsupporting

confidence: 93%

5-HMF attenuates striatum oxidative damage via Nrf2/ARE signaling pathway following transient global cerebral ischemia

Hong-fang

Wang

et al. 2017

Cell Stress and Chaperones

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Recent studies have shown 5-hydroxymethyl-2-furfural (5-HMF) has favorable biological effects, and its neuroprotection in a variety of neurological diseases has been noted. Our previous study showed that treatment of 5-HMF led to protection against permanent global cerebral ischemia. However, the underlying mechanisms in cerebral ischemic injury are not fully understood. This study was conducted to investigate the neuroprotective effect of 5-HMF and elucidate the nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) signaling pathway mechanism in the striatum after transient global cerebral ischemia. C57BL/6 mice were subjected to bilateral common carotid artery occlusion for 20 min and sacrificed 24 h after reperfusion. 5-HMF (12 mg/kg) or an equal volume of vehicle was intraperitoneally injected 30 min before ischemia and 5 min after the onset of reperfusion. At 24 h after reperfusion, neurological function was evaluated by neurological disability status scale, locomotor activity test and inclined beam walking test. Histological injury of the striatum was observed by cresyl violet staining and terminal deoxynucleotidyl transferase (TdT)-mediated dNTP nick end labeling (TUNEL) staining. Oxidative stress was evaluated by the carbonyl groups introduced into proteins, and malondialdehyde (MDA) levels. An enzyme-linked immunosorbent assay (ELISA)-based measurement was used to detect Nrf2 DNA binding activity. Nrf2 and its downstream ARE pathway protein expression such as heme oxygenase-1, NAD (P)H:quinone oxidoreductase 1, glutamate-cysteine ligase catalytic subunit and glutamate-cysteine ligase modulatory subunit were detected by western blot. Our results showed that 5-HMF treatment significantly ameliorated neurological deficits, reduced brain water content, attenuated striatum neuronal damage, decreased the carbonyl groups and MDA levels, and activated Nrf2/ARE signaling pathway. Taken together, these results demonstrated that 5-HMF exerted significant antioxidant and neuroprotective effects following transient cerebral ischemia, possibly through the activation of the Nrf2/ARE signaling pathway.

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Section: Discussionsupporting

confidence: 93%

5-HMF attenuates striatum oxidative damage via Nrf2/ARE signaling pathway following transient global cerebral ischemia

Hong-fang

Wang

et al. 2017

Cell Stress and Chaperones

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“…In this study we applied ELF-MF (50 Hz, 0.5 mT) for 7 days in gerbils submitted to 10-min global cerebral ischemia and measured oxidative stress parameters in distinct brain structures (forebrain cortex, striatum and hippocampus) on the 7 th (immediate effect of ELF-MF) and 14 th day after reperfusion (delayed effect of ELF-MF). These results are part of our comprehensive investigations concerning the effects of ELF-MF in animals with experimentally induced cerebral ischemia [19], [20] and contribute to the explanation of spatial and temporal patterns of oxidative stress in the brain of these animals.…”

Section: Introductionmentioning

confidence: 75%

“…As the source of the alternating MF was used an electromagnet whose detailed characteristics are given in Rauš et al [19]. It was placed in an isolated room with the same temperature, humidity, light intensity and cycle like in the vivarium.…”

Section: Methodsmentioning

confidence: 99%

Extremely Low Frequency Magnetic Field (50 Hz, 0.5 mT) Reduces Oxidative Stress in the Brain of Gerbils Submitted to Global Cerebral Ischemia

et al. 2014

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Magnetic field as ecological factor has influence on all living beings. The aim of this study was to determine if extremely low frequency magnetic field (ELF-MF, 50 Hz, 0.5 mT) affects oxidative stress in the brain of gerbils submitted to 10-min global cerebral ischemia. After occlusion of both carotid arteries, 3-month-old gerbils were continuously exposed to ELF-MF for 7 days. Nitric oxide and superoxide anion production, superoxide dismutase activity and index of lipid peroxidation were examined in the forebrain cortex, striatum and hippocampus on the 7th (immediate effect of ELF-MF) and 14th day after reperfusion (delayed effect of ELF-MF). Ischemia per se increased oxidative stress in the brain on the 7th and 14th day after reperfusion. ELF-MF also increased oxidative stress, but to a greater extent than ischemia, only immediately after cessation of exposure. Ischemic gerbils exposed to ELF-MF had increased oxidative stress parameters on the 7th day after reperfusion, but to a lesser extent than ischemic or ELF-MF-exposed animals. On the 14th day after reperfusion, oxidative stress parameters in the brain of these gerbils were mostly at the control levels. Applied ELF-MF decreases oxidative stress induced by global cerebral ischemia and thereby reduces possible negative consequences which free radical species could have in the brain. The results presented here indicate a beneficial effect of ELF-MF (50 Hz, 0.5 mT) in the model of global cerebral ischemia.

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“…Our previous results have shown that ELF‐MF, among others, has influence on motor behavior (Janać et al, ; Rauš et al, ), oxidative stress parameters in the brain (Jelenković et al, ; Selaković et al, ), the affinity and density of serotonin receptors in the prefrontal cortex (Janać et al, ). Having in mind that central control of GC secretion is regulated by neurosecretory neurons in the hypothalamic PVN we wanted to explore what influence ELF‐MF could have on ACTH cells.…”

Section: Introductionmentioning

confidence: 99%

Short- and long-term exposure to alternating magnetic field (50 Hz, 0.5 mT) affects rat pituitary ACTH cells: Stereological study

Balind

Manojlović‐Stojanoski

Milošević

et al. 2014

Environ. Toxicol.

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The aim of the present study was to determine does extremely low frequency magnetic field (ELF-MF, 50 Hz, 0.5 mT) affect pituitary adrenocorticotroph (ACTH) cells in adult animals. We performed two series of experiments: (1) short-term exposure of 3-month-old rats to ELF-MF for 1 and 7 days, and (2) long-term exposure of rats to ELF-MF from their conception to 3 months of age. Stereological study was performed on immunolabeled pituitary ACTH cells. The total number and volume of ACTH cells, the volume of their nuclei and pituitary volume were measured. ELF-MF exposure for 1 day significantly decreased total number and volume of ACTH cells, the volume of their nuclei, as well as pituitary volume. ELF-MF exposure for 7 days significantly reduced only the volume of ACTH cells. Life-long exposure to ELF-MF induced decrease in the volume of ACTH cells and pituitary volume. We can conclude that the applied ELF-MF has a strong influence on morphometrical parameters of the pituitary ACTH cells and could be considered as a stressogenic factor.

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Extremely low frequency magnetic field induced changes in motor behaviour of gerbils submitted to global cerebral ischemia

Cited by 21 publications

References 45 publications

5-HMF attenuates striatum oxidative damage via Nrf2/ARE signaling pathway following transient global cerebral ischemia

5-HMF attenuates striatum oxidative damage via Nrf2/ARE signaling pathway following transient global cerebral ischemia

Extremely Low Frequency Magnetic Field (50 Hz, 0.5 mT) Reduces Oxidative Stress in the Brain of Gerbils Submitted to Global Cerebral Ischemia

Short- and long-term exposure to alternating magnetic field (50 Hz, 0.5 mT) affects rat pituitary ACTH cells: Stereological study

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