Extremely high frequency electromagnetic fields at low power density do not affect the division of exponential phase Saccharomyces cerevisiae cells

Gos, Pascal; Eicher, Bernhard; Kohli, Jürg; Heyer, Wolf Dietrich

doi:10.1002/(sici)1521-186x(1997)18:2<142::aid-bem7>3.0.co;2-2

Cited by 48 publications

(27 citation statements)

References 25 publications

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“…adaptive responses to different environmental pollutants, toxic chemicals, heavy metals, and influence of electromagnetic fields [22][23][24], where the importance of their conclusions was also expanded to human cells. These studies were carried out on exponentially growing cells, which do not resemble human cells.…”

Section: Resultsmentioning

confidence: 99%

Saccharomyces cerevisiae in the stationary phase as a model organism — characterization at cellular and proteome level

Zakrajšek

Raspor

Jamnik

2011

Journal of Proteomics

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Section: Resultsmentioning

confidence: 99%

Saccharomyces cerevisiae in the stationary phase as a model organism — characterization at cellular and proteome level

Zakrajšek

Raspor

Jamnik

2011

Journal of Proteomics

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“…The design of concurrent sham controls is particularly important to rule out effects caused by potential variation of parameters that cannot be reproduced accurately. For a detailed discussion of this point see Gos et al [1997]. Sham/ sham control experiments (see Figures 3 and 4, Tables 2 and 3) and direct measurements of ®eld parameters and temperature assured the absence of relevant differences between the two chambers.…”

Section: Resultsmentioning

confidence: 99%

No mutagenic or recombinogenic effects of mobile phone fields at 900 MHz detected in the yeastSaccharomyces cerevisiae

Gos¹,

Eicher

Kohli³

et al. 2000

Bioelectromagnetics

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Both actively growing and resting cells of the yeast Saccharomyces cerevisiae were exposed to 900-MHz fields that closely matched the Global System for Mobile Communication (GSM) pulsed modulation format signals for mobile phones at specific absorption rates (SAR) of 0.13 and 1.3 W/kg. Two identical anechoic test chambers were constructed to perform concurrent control and test experiments under well-controlled exposure conditions. Using specific test strains, we examined the genotoxic potential of mobile phone fields, alone and in combination, with a known genotoxic compound, the alkylating agent methyl methansulfonate. Mutation rates were monitored by two test systems, a widely used gene-specific forward mutation assay at CAN1 and a wide-range assay measuring the induction of respiration-deficient (petite) clones that have lost their mitochondrial function. In addition, two further assays measured the recombinogenic effect of mobile phone fields to detect possible effects on genomic stability: First, an intrachromosomal, deletion-formation assay previously developed for genotoxic screening; and second, an intragenic recombination assay in the ADE2 gene. Fluctuation tests failed to detect any significant effect of mobile phone fields on forward mutation rates at CAN1, on the frequency of petite formation, on rates of intrachromosomal deletion formation, or on rates of intragenic recombination in the absence or presence of the genotoxic agent methyl methansulfonate.

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“…Phenotypic variations induced by genetic mutations are not always stable and careful screening and checking for stability are required (Gos et al 1997;Song et al 2008;Xu et al 2009). The mutagenesis approach must be efficient and simple to perform as it involved selection of rare individuals from population samples.…”

Section: Discussionmentioning

confidence: 99%

Mutational approach for N2-fixing and P-solubilizing mutant strains of Klebsiella pneumoniae RSN19 by microwave mutagenesis

Zhang

Shi

et al. 2010

World J Microbiol Biotechnol

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Nitrogen (N) fixing Klebsiella pneumoniae RSN19 has high inorganic phosphorus (P) solubilizing capability, but its N2-fixing capability is limited. In order to acquire a P-solubilizing mutant strain with high efficiency N-fixing capability, different microwave irradiation intensities and durations were tested on RSN19 in an attempt to produce mutants with improved N2-fixation and P-solubilization capabilities. The effect of microwave irradiation power and time were studied and the microwave mutagenesis parameters were optimized. Nitrogenase activity was tested on the mutant strains by acetylene reduction method; and their P-solubilizing capability and genetic stability were determined. The results indicated that the best conditions for microwave mutagenesis that produced better performed mutant strains were 250W, 36 s. Under these conditions a maximum positive mutation rate of 1.66% was obtained, resulting in five genetically stable strains with promoted nitrogenase activity which was designated as RSM-219, RSM-206, RSM-224, RSM-225 and RSM-275. Subculture tests showed that RSM-219 and RSM-206 were genetically stable mutant strains with higher nitrogenase activity and phosphate solubilizing capabilities than the original strain. Both RSM-219 and RSM-206 performed better than the original strain under N-free conditions when supplied with calcium phosphate only, and produced greater increases in the biomass of alfalfa seedlings.

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Extremely high frequency electromagnetic fields at low power density do not affect the division of exponential phase Saccharomyces cerevisiae cells

Cited by 48 publications

References 25 publications

Saccharomyces cerevisiae in the stationary phase as a model organism — characterization at cellular and proteome level

Saccharomyces cerevisiae in the stationary phase as a model organism — characterization at cellular and proteome level

No mutagenic or recombinogenic effects of mobile phone fields at 900 MHz detected in the yeastSaccharomyces cerevisiae

Mutational approach for N2-fixing and P-solubilizing mutant strains of Klebsiella pneumoniae RSN19 by microwave mutagenesis

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