Extreme genetic differentiation among the remnant populations of marble trout (Salmo marmoratus) in Slovenia

Abstract

Populations of the marble trout (Salmo marmoratus) have declined critically due to introgression by brown trout (Salmo trutta) strains. In order to define strategies for long-term conservation, we examined the genetic structure of the 8 known pure populations using 15 microsatellite loci. The analyses reveal extraordinarily strong genetic differentiation among populations separated by < 15 km, and extremely low levels of intrapopulation genetic variability. As natural recolonization seems highly unlikely, appropriate management and conservation strategies should comprise the reintroduction of pure populations from mixed stocks (translocation) to avoid further loss of genetic diversity.

Brown Trout-Salmon Hybrids in Bulgarian Rivers are not a Red Herring

“…The region bordered by primer pair is homologous between several different salmonid species, among which are the Atlantic salmon (S. salar), the brown trout (S. trutta fario), the marble trout (S. marmoratus) and others (4,7,14). the distribution of the allele length is characteristic of the different species and has been shown to serve as a marker for origin and/ or species affiliation (2, 13, 14).…”

Re‐evaluation ofSalmodata by Gridelli (1936)—description of stocking, hybridization and repopulation in the River Soča basin

“…The data obtained were analysed using Genetix version 4.05.2 (Belkhir et al 2004). Alleles characteristic for S. marmoratus were identified comparing genetically pure S. marmoratus populations (Fumagalli et al 2002) with S. trutta from hatcheries and Danubian drainage. Microsatellites with alleles 200, 202 and 212 (BFRO001), 124 (BFRO002) and 153,157,161,163,167 and 185 (Ssa197) were selected as best for estimating percentage of S. marmoratus alleles (Jug 2002).…”

“…S. marmoratus was present in the rivers of northern and eastern Adriatic drainages (Povž et al 1996), but only eight genetically pure populations remained (Fumagalli et al 2002). Since the beginning of a repopulation project in 1993 the upper Soča basin has been stocked exclusively with genetically pure S. marmoratus and the introduction of non-native trout in the entire Adriatic basin in Slovenia has been forbidden since 1996.…”

Microsatellite analysis as a tool for discriminating an interfamily hybrid between olive flounder and starry flounder

“…Several molecular markers such as alloenzymes (Berrebi et al, 2000), mitochondrial DNA (Snoj et al, 2000), microsatellites (Fumagalli et al, 2002), random amplified polymorphic DNA (RAPD; Jug et al, 2004), and single nucleotide polymorphisms (Harwood and Phillips, 2011) have been used for the discrimination of hybrids and parentage analysis in fish. Although each method has advantages and drawbacks in their application, the microsatellite markers are highly useful because they are abundant, evenly distributed, and highly polymorphic.…”