Extrato etanólico da casca de fruta de Caryocar brasiliense promove a morte e o controle do ciclo celular em células osteossarcoma caninas

Nepomuceno, Leandro Lopes; Soares, Nayane Peixoto; Borges, Juliana Carvalho de Almeida; Vieira, Vanessa de Souza; Pereira, Dayane Kelly Sabec; Pereira, Kleber Fernando; Cruz, Vanessa de Sousa; Ferreira, Jorge Luís; Araújo, Eugênio Gonçalves de

doi:10.33448/rsd-v9i10.9194

Cited by 3 publications

(7 citation statements)

References 26 publications

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“…The outcome of this study disagrees with similar studies that evaluated the cytotoxicity of other plant extracts on cancer cells by measuring cell proliferation and expression of oncogenes (37,40,45,46) . This warrants precaution when administering plants and their derivatives as phytotherapeutics, whose efficacy and safety are assured almost instantaneously because they are native and organic.…”

Section: Discussioncontrasting

confidence: 99%

“…We qualitatively and semi-quantitatively evaluated the expression levels of mutated p53 protein, Ki-67, and Bcl-2 using automated immunocytochemistry (27,37) . The data revealed relatively strong expression levels of mutated p53 protein and Ki-67, especially in the group treated with 1000 μM of the extract.…”

Section: Discussionmentioning

confidence: 99%

“…The mutated p53 protein was found in and thus affected the cellular proliferation of every experimental group; its expression is a reliable marker of proliferation and tumor progression because the mutation in the TP53 gene changes the structure of p53 protein, which loses its ability to repair DNA or induce tumor cell death (38) (39) . Ki-67 also indicates potential malignancy potential in various types of neoplasms, including canine OS cells (37,(40)(41)(42) .…”

Section: Discussionmentioning

confidence: 99%

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Turmeric ethanol extract (Curcuma longa L.) reduces apoptosis and promotes canine osteosarcoma cell proliferation

et al. 2022

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Curcuma longa L., also known as turmeric, has been widely studied for its various therapeutic properties, including antineoplastic action. The ethanolic extract of the plant contains several phenolic compounds, especially curcumin. Osteosarcoma is a predominant bone tumor in dogs and humans, characterized by high metastatic potential and an unfavorable prognosis. The aim of this study was to investigate the effects of turmeric ethanol extract on canine osteosarcoma cells from established culture. The cells were cultured and treated with different curcumin concentrations (0, 10 μM, 20 μM, 50 μM, 100 μM, and 1000 μM) and exposure times (24h, 48h, and 72h). We first performed tetrazolium reduction technique (MTT) assay and calculated IC50. An immunocytochemistry assay was performed after extract treatment to verify the expression of mutated p53 and therefore study the proliferative potential of malignant cells; Bcl-2 and Ki-67 were used to assess apoptosis and the degree of malignancy, respectively. The extract enhanced the proliferation of canine osteosarcoma cells, reaching 3,819.74% at 50 μM of curcumin. The extract also significantly altered the expression of mutated p53 and Ki-67 proteins but not that of Bcl-2, suggesting that it did not induce this antiapoptotic pathway. Overall, these results are prerequisite to better understanding how natural compounds such as turmeric ethanolic extract affect cell proliferation and could be used to treat various diseases.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Turmeric ethanol extract (Curcuma longa L.) reduces apoptosis and promotes canine osteosarcoma cell proliferation

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“…Herein, the ratio of positive staining of mutant p53 was higher in the groups treated with EBFG, with a significant difference in the EIS of that protein, according to the increased EBFG concentration. The opposite result was found in D17 cells treated with beta-lapachone [ 2 ], curcumin [ 40 ], and the ethanol extract of pequi peel [ 41 ], where the expression of mutant p53 decreased with increasing dosages and exposure time.…”

Section: Discussionmentioning

confidence: 99%

Crude extract of Jatobá leaves promotes canine osteosarcoma cell D17 proliferation

et al. 2022

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Background and Aim: New substances for neoplasm treatment have to be carefully studied to minimize adverse effects and prevent disease progression stimulation. Jatobá is a typical tree of the Cerrado and Caatinga biome, with antifungal, antimicrobial, larvicide, antioxidant, and antiproliferative properties. This study aimed to investigate the action of the crude extract of Jatobá leaves (EBFJ) on canine osteosarcoma (CO) cells and analyze the expression of biomarkers in neoplasm progression. Materials and Methods: D17 cells were cultured and subjected to treatment with EBFJ at different concentrations (10 μg/ mL; 100 μg/mL; 1000 μg/mL; 2000 μg/mL; and 5000 μg/mL) and exposure times (24 h, 48 h, and 72 h). The tetrazolium reduction assay and the immunocytochemistry technique, with anti-Bcl2, anti-p53, and anti-Ki-67 antibodies, were used to observe the effect of the extract on cell proliferation. Results: Doses of 2000 μg and 5000 μg had cell viability of 300.80% and 361.84%, respectively. The extract did not show significant cytotoxicity of samples with the control group. The confluence of cells, the number of labeled cells, and the expression of Bcl2, Ki-67, and p53 were higher in the groups treated with EBFJ, with a statistical difference from the group without treatment. Conclusion: EBFJ was not cytotoxic and had a proliferative effect on CO D17 cells. The confluence of cells, the number of labeled cells, and the expression of Bcl2, Ki-67, and p53 were higher in the groups treated with the extract.

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“…O mesmo aconteceu com o resultado da Ki-67. A técnica de imunocitoquímica automatizada permitiu avaliar a expressão das proteínas p53, Ki-67 e Bcl-2 por meio de contagem semiquantitativa e qualitativa, tendo sido validada por Fedchenko & Reifenrath e replicada desde então por pesquisadores da área (27,37) . Enfatiza-se que os resultados da imunocitoquímica não tem objetivo de nova contagem do total de células, mas de determinar o escore para o percentual de células marcadas pelos respectivos anticorpos e inferir diferenças de expressão das proteínas estudadas entre os grupos experimentais.…”

Section: Discussionunclassified

Extrato etanólico de açafrão (Curcuma longa L.) reduz apoptose e promove proliferação de células de osteossarcoma canino

et al. 2022

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Resumo A Curcuma longa L., planta conhecida popularmente como açafrão, tem sido amplamente estudada por suas diversas propriedades terapêuticas, incluindo a ação antineoplásica. O extrato etanólico da planta contém diversos compostos fenólicos, com destaque para a curcumina. O osteossarcoma é um tumor ósseo predominante em cães e humanos, caracterizado por apresentar alto potencial metastático e prognóstico desfavorável. Procurou-se investigar os efeitos de diferentes concentrações de curcumina do extrato etanólico de açafrão sobre células de osteossarcoma canino de cultura estabelecida. As células foram cultivadas e submetidas ao tratamento com extrato com diferentes concentrações de curcumina (0, 10 μM, 20 μM, 50 μM, 100 μM e 1000 μM) e tempos de exposição (24h, 48h e 72h) pelo EEA. Inicialmente, foram realizados: técnica de redução do tetrazólio (MTT) e cálculo da IC50. Posteriormente, após o tratamento com o extrato, realizou-se o ensaio de imunocitoquímica para verificar a expressão de p53 mutada e estudar o potencial proliferativo das células malignas; Bcl-2, com intuito de averiguar o estímulo de via antiapoptótica; e o marcador Ki-67, que sinaliza aumento no grau de malignidade. O extrato promoveu proliferação de células de osteossarcoma canino, com incremento de até 3819,74% na concentração de 50μM de curcumina. O composto também alterou a expressão das proteínas p53 mutante e Ki-67 significativamente, mas não alterou a expressão de Bcl-2, mostrando que não induziu a via antiapoptótica mediada por esta. Estes resultados demonstram que o extrato etanólico do açafrão apresenta potencial proliferativo sobre células de osteossarcoma canino, sugerindo a necessidade de conscientização e conhecimento dos reais efeitos de determinados compostos naturais, considerados seguros ao serem utilizados como tratamento de diversas enfermidades.

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Extrato etanólico da casca de fruta de Caryocar brasiliense promove a morte e o controle do ciclo celular em células osteossarcoma caninas

Cited by 3 publications

References 26 publications

Turmeric ethanol extract (Curcuma longa L.) reduces apoptosis and promotes canine osteosarcoma cell proliferation

Turmeric ethanol extract (Curcuma longa L.) reduces apoptosis and promotes canine osteosarcoma cell proliferation

Crude extract of Jatobá leaves promotes canine osteosarcoma cell D17 proliferation

Extrato etanólico de açafrão (Curcuma longa L.) reduz apoptose e promove proliferação de células de osteossarcoma canino

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