Extrahepatische Wirkung des Insulins Beim Zuckerverbrauch

Frank, Elizabeth L.; Nothmann, M.; Wagner, A.

doi:10.1007/bf01850072

Cited by 14 publications

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“…There has been a number of reports on the effects of insulin on arteriovenous or on capillaryvenous differences in glucose concentration in man and in laboratory animals since the original publications by Frank, Nothmann and Wagner (15) and by Cori and Cori (16). In all but one, insulin was administered in such fashion as to alter arterial glucose concentration.…”

Section: Discussionmentioning

confidence: 99%

Effect of Insulin on Carbohydrate Metabolism and on Potassium in the Forearm of Man*

Andres¹,

Baltzan²,

Cader³

et al. 1962

J. Clin. Invest.

150

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We have described a technique for quantifying skeletal muscle metabolism in man in. situ by measing blood flow through the forearm and appropriate arteriovenous differences in concentration of selected metabolites ( 1 ). The technique lends itself to analysis of local effects of hormones and other possibly potent agents. The agent can be injected into the brachial artery at constant rate with the solution of Evans blue dye used to measure blood flow. The quantity of the agent injected can be sufficiently large to exert a measurable effect in the forearm but sufficiently small so that, upon approximately 100-fold dilution when forearm venous effluent mixes with blood in the heart and great vessels, there is no measurable systemic effect. There are, therefore, no detectable systemic counter-regulatory processes set in motion by the agent to complicate the primary response of the forearm to the agent itself.It is the purpose of this report to describe the effects on forearm muscle metabolism of insulin administered in this fashion. We sought information concerning the following two problems. 1) Only a minor fraction of oxygen consumed by forearm muscle at rest can be accounted for by dissimilation of glucose abstracted from arterial blood (1). Is this because resting muscle is so poorly permeable to glucose? If glucose uptake is increased, say by exhibition of insulin, is oxidation of glucose enhanced necessarily?2) Do the temporal and molar relations between glucose uptake and potassium uptake by muscle in response to insulin suggest that insulin-induced * These studies were aided by Contract Nonr-248 (34) potassium movement is or is not secondary to the effect of insulin on glucose? METHODS Net uptake (or output) of a metabolite by forearm tissues was calculated by the equation 0 = F(A-V), where 0 is uptake (or output) of the metabolite in units of mass per minute per 100 ml of forearm, F is blood (or plasma) flow through the forearm in units of ml per minute per 100 ml of forearm, and A and V are concentrations of the metabolite in arterial and in venous blood (or plasma), respectively, in units of mass per milliliter. The technique was as described previously (1). Briefly, a double-lumen needle was inserted into the brachial artery. A polyethylene catheter was threaded into an antecubital vein and passed distally and deeply so that it sampled blood draining muscle but also, undoubtedly, adipose tissue interspersed among muscle. A second catheter was threaded into a more superficial vein, sampling mainly from skin and subcutaneous adipose tissue. Blood flow was measured by the indicator-dilution method in which Evans blue dye was injected continuously at constant rate into the brachial artery (2).Ten young adult subjects, 6 men and 4 women, were studied. All were medical students or laboratory personnel in good health and near ideal body weight. Dietary history of carbohydrate intake was normal. No food was taken after 7 p.m. on the evening preceding the experiment. The first blood samples were obtained betwee...

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Section: Discussionmentioning

confidence: 99%

Effect of Insulin on Carbohydrate Metabolism and on Potassium in the Forearm of Man*

Andres¹,

Baltzan²,

Cader³

et al. 1962

J. Clin. Invest.

150

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“…This was done in evisceration experiments in dogs. Frank, Nothmann and Wagner (2,3), working with rabbits and pancreatectomized dogs, claimed a local peripheral action of insulin following its injection into a femoral artery. In vitro, it has been shown that the glucose uptake of the rat diaphragm is increased under the action of added insulin (4)(5)(6).…”

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Effect on Femoral a-v Glucose Difference of Insulin Injected Into an Antecubital Vein and Into a Femoral Artery 1

Bell¹,

Burns²

1952

J. Clin. Invest.

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There still exists considerable controversy with respect to the importance of the liver as compared to the peripheral tissues (skeletal muscle) in the homeostasis of blood sugar levels. The place and mechanism of action of insulin remain unclear.Mann and Magath (1) are credited with first establishing the peripheral action of insulin apart from the liver. This was done in evisceration experiments in dogs. Frank, Nothmann and Wagner (2, 3), working with rabbits and pancreatectomized dogs, claimed a local peripheral action of insulin following its injection into a femoral artery. In vitro, it has been shown that the glucose uptake of the rat diaphragm is increased under the action of added insulin (4-6). Stadie and Zapp (7) have demonstrated that insulin is fixed to the diaphragm and cannot be washed off.The purpose of this paper is to record data demonstrating the different effect on femoral A-V glucose difference of insulin injected into a femoral artery and into an antecubital vein. METHODSConvalescent hospital patients and normal medical students served as subjects.All studies were done in the morning and the subjects had had no food for 12 to 14 hours. They were at rest for an hour prior to insulin injection. No attempt was made to control room temperature.The main femoral artery and vein were used for sampling. Samples were drawn simultaneously into heparinized syringes and immediately transferred to tubes prepared by the evaporation of 1 ml. of 3 percent sodium fluoride and these were then placed in ice. Using barium hydroxide and zinc sulfate precipitation, filtrates were made at the completion of the experiment.

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Über Insulin und seinen Wirkungsmechanismus

Staub

1927

Ergebnisse Der Inneren Medizin Und Kinderheilkunde

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Extrahepatische Wirkung des Insulins Beim Zuckerverbrauch

Cited by 14 publications

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Effect of Insulin on Carbohydrate Metabolism and on Potassium in the Forearm of Man*

Effect of Insulin on Carbohydrate Metabolism and on Potassium in the Forearm of Man*

Effect on Femoral a-v Glucose Difference of Insulin Injected Into an Antecubital Vein and Into a Femoral Artery 1

Über Insulin und seinen Wirkungsmechanismus

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