Extracts of Physalis peruviana Protect Astrocytic Cells Under Oxidative Stress With Rotenone

Abstract

The use of medicinal plants to counteract the oxidative damage in neurodegenerative diseases has steadily increased over the last few years. However, the rationale for using these natural compounds and their therapeutic benefit are not well explored. In this study, we evaluated the effect of different Physalis peruviana extracts on astrocytic cells (T98G) subjected to oxidative damage induced by rotenone. Extracts of fresh and dehydrated fruits of the plant with different polarities were prepared and tested in vitro. Our results demonstrated that the ethanolic extract of fresh fruits (EF) and acetone-dehydrated fruit extract (AD) increased cell viability, reduced the formation of reactive oxygen species (ROS) and preserved mitochondrial membrane potential. In contrast, we observed a significant reduction in mitochondrial mass when rotenone-treated cells were co-treated with EF and AD. These effects were accompanied by a reduction in the percentage of cells with fragmented/condensed nuclei and increased expression of endogenous antioxidant defense survival proteins such as ERK1/2. In conclusion, our findings suggest that ethanolic and acetone extracts from P. peruviana are potential medicinal plant extracts to overcome oxidative damage induced by neurotoxic compounds.

Astrocytic Oxidative/Nitrosative Stress Contributes to Parkinson’s Disease Pathogenesis: The Dual Role of Reactive Astrocytes

“…Additionally, rotenone induced increased astrocyte proliferation and morphological changes in astrocytes in the SN of rats, suggesting that reactive astrogliosis may serve a mechanism of rotenone toxicity and associated pathogenesis [128]. Rotenone also induced ROS production and decreased antioxidant levels in cultured astrocytes, leading to the impairment of mitochondrial membrane potential and astrocytic toxicity in T98G human astrocytes [129], indicating that astrocytic oxidative/nitrosative stress plays a critical role in rotenone-induced toxicity.…”

Physalis peruviana L. Pulp Prevents Liver Inflammation and Insulin Resistance in Skeletal Muscles of Diet-Induced Obese Mice

“…Physalis peruviana L. (PP) is distributed across South America; it is an abundant source of phenolic components, like coumarin, kaempferol, quercetin, and ascorbic acid, which have several biological activities including the prevention of some metabolic abnormalities associated with obesity [17]. Some of the beneficial effects of PP are described in different reports; PP extract reduced the formation of reactive oxygen species (ROS) and preserved mitochondrial membrane potential in astrocytes [18]; it attenuated airway inflammation associated with chronic obstructive pulmonary disease by inhibiting ROS production and activating antioxidant defense of the epithelial cells [19]. In the liver, the PP juice reduced CCl 4 -associated with liver injury, decreasing the lipoperoxidation level [20].…”

Physalis peruviana L.: perspectiva de aplicabilidade no tratamento de câncer

“…O extrato aquoso do cabo foi utilizado frente a linhagem de colón e observou efeito antiproliferativo, com um acúmulo das células na fase G1, induziu a apoptose, a ativação da caspase 3, assim como observado no extrato de dióxido de carbono supercrítico (Wu et al, 2009), e ação antioxidante (Areiza-Mazo;Maldonado & Rojano, 2013). Ainda no estudo com o dióxido, Wu e colaboradores ( 2009) também observaram o aumento na expressão da Bax e p53, parada do ciclo na fase S e fragmentação do DNA nas células de pulmão.…”