Extracts of Actinidia arguta stems inhibited LPS-induced inflammatory responses through nuclear factor–κB pathway in Raw 264.7 cells

Kim, Hae-Young; Hwang, Kwang Woo; Park, Soyoung

doi:10.1016/j.nutres.2014.08.019

Cited by 36 publications

(23 citation statements)

References 25 publications

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“…In the MTT assay, hardy kiwifruit extracts showed the most inhibition of Hep3B viability among the tested cancer cells, suggesting the proliferation inhibitory potential of the extracts. In addition to the clinical efficacy of A. aruguta extracts for atopic dermatitis (Kim et al, 2009) and inflammation (Kim, Hwang, & Park, 2014), we suggest possible roles of the fruit extracts for cancer inhibition, specifically against Hep3B cells.…”

Section: Anticancer Properties Of the Fruit Extractsmentioning

confidence: 81%

Inhibition of hardy kiwifruit (Actinidia aruguta) ripening by 1-methylcyclopropene during cold storage and anticancer properties of the fruit extract

et al. 2016

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Hardy kiwifruits (Actinidia arguta) were treated with 20 μl/l 1-methylcyclopropene (1-MCP) for 16 h at 10 °C and subsequently stored at 1 ± 0.5 °C. Anticancer properties of the fruit extracts were tested against five different human cancer cells. The hardy kiwifruits, without 1-MCP treatment, showed increases in both respiration and ethylene production rates during fruit storage. The 1-MCP treatment remarkably inhibited fruit ripening by reducing respiration and ethylene production. Fruits with the 1-MCP treatment could be stored for up to 5 weeks by maintaining higher fruit firmness, ascorbic acid and total phenolic contents compared to the control. The hardy kiwifruit extracts showed anti-proliferative effects to Hep3B and HeLa cells but not to HT29, HepG2 and LoVo cells. These results suggest that the application of 1-MCP at harvest effectively delayed the ripening process of the fruits, and the fruit extract had beneficial effects for the prevention of human cancer growth.

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Section: Anticancer Properties Of the Fruit Extractsmentioning

confidence: 81%

Inhibition of hardy kiwifruit (Actinidia aruguta) ripening by 1-methylcyclopropene during cold storage and anticancer properties of the fruit extract

et al. 2016

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“…Under normal resting conditions, NF- κ B and IkBa aggregate into a trimer within the cytoplasm. In response to LPS, IkBa is predominately phosphorylated and subsequently degraded, which frees NF- κ B-p65 into the nucleus, and gene transcription of various proinflammatory factors is initiated [28, 29]. …”

Section: Discussionmentioning

confidence: 99%

Andrographolide Inhibits Inflammatory Cytokines Secretion in LPS‐Stimulated RAW264.7 Cells through Suppression of NF‐κB/MAPK Signaling Pathway

Tang

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Andrographolide, the main active component extracted from Andrographis paniculata (Burm.f.) Wall. ex Nees, exerts anti-inflammatory effects; however, the principal molecular mechanisms remain unclear. The objective of this study was to investigate the molecular mechanisms of Andrographolide in modifying lipopolysaccharide- (LPS-) induced signaling pathway in RAW264.7 cells. An in vitro model of inflammation was induced by LPS in mouse RAW264.7 cells in the presence of Andrographolide. The concentration and expression levels of proinflammatory cytokines were determined by an enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRT-PCR), respectively. The nuclear level of NF-κB was measured by an electrophoretic mobility shift assay (EMSA). The expression levels of NF-κB, p38, ERK, and JNK were determined by western blot. Andrographolide dose-dependently inhibited the release and mRNA expression of TNF-α, IL-6, and IL-1β in LPS-stimulated RAW264.7 cells. The nuclear level of p65 protein was decreased in Andrographolide treatment group. Western blot analysis showed that Andrographolide suppressed LPS-induced NF-κB activation and the phosphorylation of IkBa, ERK1/2, JNK, and p38. These results suggest that Andrographolide exerts an anti-inflammatory effect by inhibiting the activation of NF-κB/MAPK signaling pathway and the induction of proinflammatory cytokines.

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“…Several studies reported similar anti‐inflammation effects without the alteration of COX2. Kim, Hwang, & Park, (2014) showed chloroform layer of Actinidia arguta stems extract did not alter the COX‐2 level, but it strongly reduced NO production, proinflammatory cytokines in both protein and mRNA expression levels, and inhibited activation of NF‐κB and mitogen‐activated protein kinase (MAPKs). Likewise, Park, Kwon, & Sung, (2009) reported aloin could reduce NO production and level of proinflammatory cytokines, but had no effect on COX‐2 expression.…”

Section: Discussionmentioning

confidence: 99%

Anti‐inflammatory and anti‐insulin resistance activities of aqueous extract from Anoectochilus burmannicus

Budluang

Pitchakarn

Ting

et al. 2016

Food Science & Nutrition

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This study investigated biological activities including antioxidative stress, anti‐inflammation, and anti‐insulin resistance of Anoectochilus burmannicus aqueous extract (ABE). The results showed abilities of ABE to scavenging DPPH and ABTS free radicals in a dose‐dependent manner. Besides, ABE significantly reduced nitric oxide (NO) production in the lipopolysaccharide (LPS)‐treated RAW 264.7 via inhibition of mRNA and protein expressions of nitric oxide synthase (iNOS). The LPS‐induced mRNA expressions of cyclooxygenase‐2 (COX‐2) and interleukin 1β (IL‐1β) were suppressed by ABE. Moreover, ABE exerted anti‐insulin resistance activity as it significantly improved the glucose uptake in tumor necrosis factor (TNF)‐α treated 3T3‐L1 adipocytes. In addition, ABE at the concentration of up to 200 μg/mL was not toxic to human peripheral blood mononuclear cells (PBMCs) and did not induce mutations. Finally, the results of our study suggest the potential use of A. burmannicus as anti‐inflammatory, anti‐insulin resistance agents, or food supplement for prevention of chronic diseases.

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Extracts of Actinidia arguta stems inhibited LPS-induced inflammatory responses through nuclear factor–κB pathway in Raw 264.7 cells

Cited by 36 publications

References 25 publications

Inhibition of hardy kiwifruit (Actinidia aruguta) ripening by 1-methylcyclopropene during cold storage and anticancer properties of the fruit extract

Inhibition of hardy kiwifruit (Actinidia aruguta) ripening by 1-methylcyclopropene during cold storage and anticancer properties of the fruit extract

Andrographolide Inhibits Inflammatory Cytokines Secretion in LPS‐Stimulated RAW264.7 Cells through Suppression of NF‐κB/MAPK Signaling Pathway

Anti‐inflammatory and anti‐insulin resistance activities of aqueous extract from Anoectochilus burmannicus

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