Extracts from the Mongolian traditional medicinal plants Dianthus versicolor Fisch. and Lilium pumilum Delile stimulate bile flow in an isolated perfused rat liver model

Obmann, Astrid; Tsend-Ayush, Damba; Thalhammer, Theresia; Zehl, Martin; Vo, Thanh Phuong Nha; Purevsuren, Sodnomtseren; Natsagdorj, Damdinsuren; Narantuya, Samdan; Kletter, Christa; Glasl, Sabine

doi:10.1016/j.jep.2010.07.029

Cited by 22 publications

(19 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The plant has been shown to contain a series of pentacyclic triterpenoid saponins 6. Our investigations have shown that an aqueous extract and a flavonoid‐enriched fraction of D. versicolor cause an increase in bile flow in the isolated perfused rat liver 5. Bioactivity‐guided fractionation led to the isolation and structure elucidation of a number of flavonoids 7.…”

Section: Introductionmentioning

confidence: 89%

“…For phytochemical investigations, aqueous extracts of all five D. versicolor samples were prepared according to a method we had described earlier 5. Briefly, 10 g of the dried aerial parts were powdered and extracted with 250 mL water (pH 2, adjusted with trifluoroacetic acid) for 1 h at 40°C by shaking gently.…”

Section: Methodsmentioning

confidence: 99%

“…Another indication, although scientifically less studied, comprises impairments of the liver as reported by a very reputed traditional health institution 4. Support for this use was obtained by recent investigations in the model of the isolated perfused rat liver 5. According to the “Report of market research on Mongolian traditional medicinal drugs” prepared in September 2007 for the WHO 3, D. versicolor ranks among the 45 most common domestic herbal drugs traded in Mongolia.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Quantification of flavonoid glycosides in an aqueous extract from the traditional Mongolian medicinal plant Dianthus versicolorFISCH.

Obmann

Zehl

Purevsuren

et al. 2011

J of Separation Science

Self Cite

View full text Add to dashboard Cite

An HPLC-diode array detection (DAD) method was established in order to investigate dried aerial parts of Dianthus versicolor FISCH. (Caryophyllaceae), a plant used in traditional Mongolian medicine against liver impairment. Aqueous extracts were separated on an Aquasil(®) C(18) column with a linear gradient of acetonitrile (ACN) and water (adjusted to pH 2.8 with formic acid) as the mobile phase. LC-IT-MS facilitated the assignment of 26 flavonoids, among them a series of rare C-glycosylated as well as O-glycosylated derivatives, which are assumed to be the active principles. Quantification was performed and validated using isovitexin-7-O-glucoside (saponarin) as the external standard. The method showed good linear behaviour (r(2) ≥0.9999) over the investigated concentration range (0.007-3.5 mg/mL). The good precision of the method allowed the successful qualitative and quantitative analysis of flavonoid-glycosides in the aqueous extracts prepared from five different D. versicolor samples. Depending on the origin of the samples, the total flavonoid content was found to vary considerably from 0.41 to 3.30% in the aqueous extracts and from 0.07 to 0.57% in the crude drug. In addition, the relative composition of the various flavonoids was found to differ strongly. These results highlight the need for proper quality control for this herbal drug.

show abstract

Section: Introductionmentioning

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Quantification of flavonoid glycosides in an aqueous extract from the traditional Mongolian medicinal plant Dianthus versicolorFISCH.

Obmann

Zehl

Purevsuren

et al. 2011

J of Separation Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…LC-UV-MS plays an important role in the screening of phenols and flavonoids. In most cases, ESI-IT-MS n spectral information in conjunction with UV spectra allows rapid identification [69][70][71][72][73][74][75][76][77][78][79][80][81][82][83][84]. The application of TOF-MS in structural elucidation of natural products is of ever increasing interest because TOF mass analyzer with high resolving power can provide the accurate mass measurement and full scan spectral sensitivity [85].…”

Section: Phenols and Flavonoidsmentioning

confidence: 99%

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

Guo

Chen

et al. 2013

Journal of Pharmaceutical and Biomedical Analysis

161

View full text Add to dashboard Cite

“…The other mechanism is that osmotically active compounds can cause an enhanced water flow when they secreted into the bile, which is also defined as bile acid‐independent secretion. Obviously bile acid‐dependent secretion have better clinic application value since it would lead to a better digestion of fats (Obmann and others ).…”

Section: Introductionmentioning

confidence: 99%

Choleretic Activity of Turmeric and its Active Ingredients

Wang

Zhu

et al. 2016

Journal of Food Science

View full text Add to dashboard Cite

Turmeric, a rhizome of Curcumin longa L. is widely used as both a spice and an herbal medicine. The traditional use of turmeric in gastroenterology is mainly based on its choleretic activity. The aim of this study is to determine the effects of turmeric on bile flow (BF) and total bile acids (TBAs) excretion in a bile fistula rat model after acute duodenal administration. A significant dose-dependent enhancement in both BF and TBAs was detected after treatment with the turmeric decoctions which suggested the choleretic activity was bile acid-dependent secretion. In order to direct the active group of compounds, aqueous (AE), ethyl acetate (EtOAc), and petroleum ether (PE) extracts were investigated. The EtOAc and PE extracts showing high effects were purified to locate the active ingredients. Three curcuminoids (curcumin, demethoxycurcumin, and bisdemethoxycurcumin) and 2 sesquiterpenes (bisacurone B and ar-turmerone) were isolated. It was found Bisacurone B was the most potent choleretic ingredient followed by ar-turmerone, bisdemethoxycurcumin demethoxycurcumin, and then curcumin. The amounts of the active ingredients were quantitatively analyzed by high-performance liquid chromatography. The EtOAc and PE extracts had high sesquiterpenes and curcuminoids content, while the AE extract had poor content of sesquiterpenes and curcuminoids which affected neither BF nor TBAs. Based on the results of multiple linear regression analysis, the content of BIS and TUR were dominant factors (P < 0.01) of controlling BL and TBAs in EtOAC and PE extracts.

show abstract

Extracts from the Mongolian traditional medicinal plants Dianthus versicolor Fisch. and Lilium pumilum Delile stimulate bile flow in an isolated perfused rat liver model

Cited by 22 publications

References 13 publications

Quantification of flavonoid glycosides in an aqueous extract from the traditional Mongolian medicinal plant Dianthus versicolorFISCH.

Quantification of flavonoid glycosides in an aqueous extract from the traditional Mongolian medicinal plant Dianthus versicolorFISCH.

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

Choleretic Activity of Turmeric and its Active Ingredients

Contact Info

Product

Resources

About