Extractive fermentation in cloud point system for lipase production by Serratia marcescens ECU1010

Pan, Tao; Wang, Zhilong; Xu, Jian‐He; Wu, Zhenqiang; Qi, Haifeng

doi:10.1007/s00253-009-2257-4

Cited by 31 publications

(19 citation statements)

References 39 publications

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“…This represents 88% of the total prodigiosin produced while the extractive phase partitioned 93% of the biomass to the dilute phase (partition coefficient, Kp = 0.074). Pan et al have reported this biomass clearance in the extractive fermentations, obtaining surfactant‐rich cell‐free phases . For successful ATPS‐based extractive fermentation, an optimal ATPS adequate for extractive partitioning needs to be selective in order to partition cells and bioproduct into opposite phases .…”

Section: Resultssupporting

confidence: 90%

“…From this study it was demonstrated (Table ) that the sole addition of 2% of triton X‐114 to a culture of MGP medium did not affect significantly ( P ‐value: 0.97) the final prodigiosin production, however, biomass increased 1 g L −1 , resulting in a higher biomass concentration but a lower production yield. Similar effects have been reported suggesting that 2% triton X‐114 may be suitable for extractive production of lipase from Serratia marcescens ECU1010 . Pan et al reported the growth of Serratia marcescens in a surfactant mixture including triton X‐114 and X‐45 at concentrations ranging from 8–32 g L −1 , but independent from the metabolites production …”

Section: Resultsmentioning

confidence: 99%

“…ISPR techniques have been reported for bioprocesses in bio‐fuel production, antibiotics, pharmaceutics, food additives, natural colorants and some industrially relevant enzymes . These strategies integrate effective separation technologies, such as gas stripping, pervaporation, diafiltration, aqueous two‐phase systems (ATPS), resin adsorption, electrodyalisis, crystallization and organic–aqueous biphasic systems into fermentation processes …”

Section: Introductionmentioning

confidence: 99%

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An integrated process for thein siturecovery of prodigiosin using micellar ATPS from a culture ofSerratia marcescens

Chávez-Castilla

Aguilar

2016

J. Chem. Technol. Biotechnol.

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BACKGROUND In situ product recovery (ISPR), or extractive fermentation, is a strategy for the primary recovery of metabolites. A micellar aqueous two‐phase system (ATPS) composed of a surfactant‐rich phase was coupled to batch and continuous fermentations for the production and in situ recovery of the red alkaloid prodigiosin from Serratia marcescens. A cloud point ATPS was formed at 24 °C using triton X‐114 and used as a production/extraction system for this metabolite, which shows therapeutic properties as antibiotic, anti‐proliferative and anti‐malarial activities. RESULTS The integration of the micellar ATPS in batch fermentation resulted in the production of 342 mg prodigiosin L−1 with a recovery of 81% in the extractive phase. When a continuous fermentation was performed, based on a cloud point system, 1.2‐fold prodigiosin content was obtained compared with batch mode and a recovery of 88% of the metabolite. This continuous operation resulted in 0.43 g of purified prodigiosin from a 1.5 L bioreactor with a concentration of 872 mg prodigiosin L−1 of extractive phase and a purity of 82%, demonstrating particular selectivity of the system for prodigiosin as a primary recovery method. This is the first report on the continuous production of this generally recognized secondary metabolite. CONCLUSION The experimental approach proposed proved that an ISPR system could lead to the establishment of a more efficient process for continuous large‐scale prodigiosin production. The proposal of a primary recovery strategy with only one bioprocess step enhances this metabolite production by lowering the inhibiting properties of prodigiosin for the microorganism. © 2016 Society of Chemical Industry

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An integrated process for thein siturecovery of prodigiosin using micellar ATPS from a culture ofSerratia marcescens

Chávez-Castilla

Aguilar

2016

J. Chem. Technol. Biotechnol.

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“…The growth of the strains using the different carbon sources was similar (Table ). Various authors report that the maximum lipase production was obtained when maximum biomass production was attained . These results suggest that lipase production is associated with growth.…”

Section: Discussionmentioning

confidence: 88%

Production and application of a thermostable lipase from Serratia marcescens in detergent formulation and biodiesel production

García‐Silvera

Martínez‐Morales

Bertrand

et al. 2017

Biotech and App Biochem

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In this study, extracellular lipase was produced by Serratia marcescens wild type and three mutant strains. The maximum lipase activity (80 U/mL) was obtained with the SMRG4 mutant strain using soybean oil. Using a 2 factorial design, the lipase production increased 1.55-fold (124 U/mL) with 4% and 0.05% of soybean oil and Triton X-100, respectively. The optimum conditions for maximum lipase activity were 50 °C and pH 8. However, the enzyme was active in a broad range of pH (6-10) and temperatures (5-55 °C). This lipase was stable in organic solvents and in the presence of oxidizing agents. The enzyme also proved to be efficient for the removal of triacylglycerol from olive oil in cotton cloth. A Box-Behnken experimental design was used to evaluate the effects of the interactions between total lipase activity, buffer pH, and wash temperatures on oil removal. The model obtained suggested that all selected factors had a significant impact on oil removal, with optimum conditions of 550 U lipase, 45 °C, pH 9.5, with 79.45% removal. Biotransformation of waste frying oil using the enzyme and in presence of methanol resulted in the synthesis of methyl esters such as methyl oleate, methyl palmitate, and methyl stearate.

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“…The highest clavulanic acid concentration was obtained in a bioreactor PEG-rich phase with yield 30% higher than in the flasks, thus demonstrating the potential of such a new process for the production and extraction in the same step (83). Pan et al (84) applied a cloud point system in an extractive fermentation, using a combination of Triton X-114 and Triton X-45. They reported a 95% recovery yield of lipase produced by Serratia marcescens.…”

Section: New Techniques Integrated With Atpssmentioning

confidence: 99%

Aqueous Two‐Phase Systems (ATPSs) for Extraction and Purification of Biomolecules and Bioparticles

Marques

Molino

Mazzola

et al. 2013

Encyclopedia of Industrial Biotechnology

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The study of biomolecules and bioparticles has achieved great advances, thus increasing the productivity with upstream improvements. Nowadays, downstream processes are the bottleneck in the production of some biopharmaceuticals, a change from the previous years' scene. Current purification platforms will reach their physical limits at some point, creating the necessity for new approaches. This article reviews an alternative method for the extraction and purification of biomolecules/bioparticles denominated as aqueous two‐phase systems (ATPSs) . Biocompatibility and readiness to scale up are some of the characteristics of ATPSs and we also discuss its application in the biotechnology field.

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Extractive fermentation in cloud point system for lipase production by Serratia marcescens ECU1010

Cited by 31 publications

References 39 publications

An integrated process for thein siturecovery of prodigiosin using micellar ATPS from a culture ofSerratia marcescens

An integrated process for thein siturecovery of prodigiosin using micellar ATPS from a culture ofSerratia marcescens

Production and application of a thermostable lipase from Serratia marcescens in detergent formulation and biodiesel production

Aqueous Two‐Phase Systems (ATPSs) for Extraction and Purification of Biomolecules and Bioparticles

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