Production and application of a thermostable lipase from Serratia marcescens in detergent formulation and biodiesel production

Abstract

In this study, extracellular lipase was produced by Serratia marcescens wild type and three mutant strains. The maximum lipase activity (80 U/mL) was obtained with the SMRG4 mutant strain using soybean oil. Using a 2 factorial design, the lipase production increased 1.55-fold (124 U/mL) with 4% and 0.05% of soybean oil and Triton X-100, respectively. The optimum conditions for maximum lipase activity were 50 °C and pH 8. However, the enzyme was active in a broad range of pH (6-10) and temperatures (5-55 °C). This lipase was stable in organic solvents and in the presence of oxidizing agents. The enzyme also proved to be efficient for the removal of triacylglycerol from olive oil in cotton cloth. A Box-Behnken experimental design was used to evaluate the effects of the interactions between total lipase activity, buffer pH, and wash temperatures on oil removal. The model obtained suggested that all selected factors had a significant impact on oil removal, with optimum conditions of 550 U lipase, 45 °C, pH 9.5, with 79.45% removal. Biotransformation of waste frying oil using the enzyme and in presence of methanol resulted in the synthesis of methyl esters such as methyl oleate, methyl palmitate, and methyl stearate.

Lipases in liquid formulation for biodiesel production: Current status and challenges

“…Lipases (triacylglycerol ester hydrolases, EC.3.1.1.3) are enzymes that have demonstrated to be useful in different hydrolytic and synthetic reactions, being the class of enzymes most widely employed in organic synthesis due to a considerable number of commercially available preparations and better stability compared with other enzymes in media containing organic solvents [66,67]. The potential of application for lipases is noticeable in the production of biofuels, leather, foods, detergents, cosmetics, fragrances, and pharmaceuticals [68,69]. Moreover, they are easily obtained from a diversity of biological sources by extraction from animal and plant tissues or cultivation of microorganisms, providing enzymes with different substrate specificities and catalytic properties [66,70].…”

Optimization of the enzymatic hydrolysis ofMoringa oleiferaLam oil using molecular docking analysis for fatty acid specificity

“…Fatty acids are a valuable group of chemical compounds used in industrial production of several bioproducts such as pharmaceuticals [1], foods [2], soaps [3], surfactants [4], lubricants [5], and biodiesel [6]. Derived from triglycerides, fatty acids from vegetable oils are more attractive as compared with fatty acids from animal fats.…”

Statistical Methodologies for the Optimization of Lipase and Biosurfactant by Ochrobactrum intermedium Strain MZV101 in an Identical Medium for Detergent Applications

“…Lipase production increased to 124 U/mL, about 1.55-fold higher than the initial ratio. The optimum medium composition and conditions were 4% soybean oil and 0.05% Triton X-100 at 50 °C and pH 8 with 200 rpm after 48 h of incubation [ 44 ]. Our study shows an inferior value for lipase production by O. intermedium strain MZV101.…”