Extractive bioconversion of poly-ϵ-caprolactone by Burkholderia cepacia lipase in an aqueous two-phase system

Chew, Pey Ling; Annuar, Mohamad Suffian Mohamad; Show, Pau Loke; Ling, Tau Chuan

doi:10.1016/j.bej.2015.04.015

Cited by 25 publications

(6 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This error is known as a random error, which is produced from the collected experimental data. 42 Figure 2c (residuals against fitted values) showed that there was no strong evidence of systematic error as shown by a random pattern of residuals. The lack of pattern observed in Figure 2d indicates that the order in which data is collected has no impact on the data collected.…”

Section: Resultssupporting

confidence: 75%

See 1 more Smart Citation

Statistical Optimization and Kinetic Modeling of Lipase-Catalyzed Synthesis of Diacylglycerol in the Mixed Solvent System of Acetone/tert-Butanol

Razak

Gew

Pérès

et al. 2021

Ind. Eng. Chem. Res.

View full text Add to dashboard Cite

Diacylglycerols have been extensively used in food, medicine, cosmetic, and pharmaceutical industries due to their outstanding properties as emulsifiers and stabilizers as well as their nutritional benefits to humans. This study investigated the optimization and kinetics of lipase-catalyzed synthesis of glycerol dioleates (GDO) through esterification of glycerol with oleic acid in a mixed solvent system of acetone/tert-butanol. Process variables, namely, solvent ratio, temperature, and enzyme loading, were optimized to acquire maximum conversion and selectivity. A high oleic acid conversion of 73% and GDO selectivity of 77% were obtained in an acetone/tert-butanol 70:30 (v/v) mixture at 45 °C and an enzyme load of 0.15 g. The bi substrates Ping-Pong Bi-Bi kinetic model was employed for initial rate determination, which was fitted to the experimental results. At an applied temperature of 45 °C, the Ping-Pong Bi-Bi model with no substrate inhibition gives the best fit with parameter values of V max = 1.21 M h–1, K m [G] = 1.81 M, and K m [OA] = 6.81 M for glycerol and oleic acid concentrations between 0.31 to 1.32 M and 1.88 to 2.13 M, respectively.

show abstract

Section: Resultssupporting

confidence: 75%

“…An outlier can be seen from the bar indicated by being slightly outside from the ±2 range (Figure b). This error is known as a random error, which is produced from the collected experimental data Figure c (residuals against fitted values) showed that there was no strong evidence of systematic error as shown by a random pattern of residuals.…”

Section: Resultssupporting

confidence: 75%

Statistical Optimization and Kinetic Modeling of Lipase-Catalyzed Synthesis of Diacylglycerol in the Mixed Solvent System of Acetone/tert-Butanol

Razak

Gew

Pérès

et al. 2021

Ind. Eng. Chem. Res.

View full text Add to dashboard Cite

show abstract

“…This process is complex, expensive and time‐consuming. PCL hydrolysis catalyzed by BCL was studied by Chew, Annuar, Show, & Ling, . The authors performed the extractive bioconversion of the polymer to its monomers and oligomers in an aqueous two‐phase system (ATPS).…”

Section: Synthesis and Degradation Of Polymersmentioning

confidence: 99%

Burkholderia cepacia lipase: A versatile catalyst in synthesis reactions

Sánchez

Tonetto

Ferreira

2017

Biotech & Bioengineering

View full text Add to dashboard Cite

The lipase from Burkholderia cepacia, formerly known as Pseudomonas cepacia lipase, is a commercial enzyme in both soluble and immobilized forms widely recognized for its thermal resistance and tolerance to a large number of solvents and short-chain alcohols. The main applications of this lipase are in transesterification reactions and in the synthesis of drugs (because of the properties mentioned above). This review intends to show the features of this enzyme and some of the most relevant aspects of its use in different synthesis reactions. Also, different immobilization techniques together with the effect of various compounds on lipase activity are presented. This lipase shows important advantages over other lipases, especially in reaction media including solvents or reactions involving short-chain alcohols.

show abstract

“…The crude microalgae biomass loading was investigated first to determine the biomass loading for determining the critical loading level of the microalgae in the LBEF system. It is noted that, biomass loading potentially affects two phase formation thus affecting the total protein yield . Previous reports also suggest that crude biomass loading of greater than 5% w/w result in decrease in protein separation efficiency .…”

Section: Results and Discussion For Optimization Of Lbef Systemmentioning

confidence: 99%

Optimization of protein extraction from Chlorella Vulgaris via novel sugaring‐out assisted liquid biphasic electric flotation system

Koyande

Chew

Lim

et al. 2019

Engineering in Life Sciences

View full text Add to dashboard Cite

Microalgae biomass has been consumed as animal feed, fish feed and in human diet due to its high nutritional value. In this experiment, microalgae specie of Chlorella Vulgaris FSP‐E was utilized for protein extraction via simple sugaring‐out assisted liquid biphasic electric flotation system. The external electric force provided to the two‐phase system assists in disruption of rigid microalgae cell wall and releases the contents of microalgae cell. This experiment manipulates various parameters to optimize the set‐up. The liquid biphasic electric flotation set‐up is compared with a control liquid biphasic flotation experiment without the electric field supply. The optimized separation efficiency of the liquid biphasic electric flotation system was 73.999 ± 0.739% and protein recovery of 69.665 ± 0.862% compared with liquid biphasic flotation, the separation efficiency was 61.584 ± 0.360% and protein recovery was 48.779 ± 0.480%. The separation efficiency and protein recovery for 5 × time scaled‐up system was observed at 52.871 ± 1.236% and 73.294 ± 0.701%. The integration of simultaneous cell‐disruption and protein extraction ensures high yield of protein from microalgae. This integrated method for protein extraction from microalgae demonstrated its potential and further research can lead this technology to commercialization.

show abstract

Extractive bioconversion of poly-ϵ-caprolactone by Burkholderia cepacia lipase in an aqueous two-phase system

Cited by 25 publications

References 25 publications

Statistical Optimization and Kinetic Modeling of Lipase-Catalyzed Synthesis of Diacylglycerol in the Mixed Solvent System of Acetone/tert-Butanol

Statistical Optimization and Kinetic Modeling of Lipase-Catalyzed Synthesis of Diacylglycerol in the Mixed Solvent System of Acetone/tert-Butanol

Burkholderia cepacia lipase: A versatile catalyst in synthesis reactions

Optimization of protein extraction from Chlorella Vulgaris via novel sugaring‐out assisted liquid biphasic electric flotation system

Contact Info

Product

Resources

About